背景与目的

我们以前的研究发现：创伤/失血性休克（T/HS）复苏后早期（3小时）肺损伤明显，雌性动物较雄性动物耐受性强。但是，T/HS后期肺损伤是否依然存在，是否具有性别差异目前仍然未知，本研究观察雌、雄动物T/HS后期肺损伤的差异。

方法

雌、雄性SD大鼠剖腹（创伤）后经颈动脉放血致T/HS或假休克（T/SS），90分钟后休克大鼠用乳酸林格氏液（RL）复苏；复苏后5天评估肺损伤的情况。

结果

T/HS后大鼠的肺通透性、肺髓过氧化物酶（MPO）水平以及支气管肺泡灌注液（BALF）蛋白浓度与血浆蛋白浓度的比值增加，雌性大鼠的上述变化与雄性大鼠相比更加显著。

结论

创伤/失血性休克5天后仍然存在肺损伤，而且有显著的性别差异，雌性大鼠肺损伤较雄性大鼠重。

Background and Aims

Previously we have documented that lung injury after trauma-hemorrhagic shock（T/HS）is significant 3 hours after resuscitation and that females are more resistant toT/HS-induced lung injury than males.However，it is not known if the lung injury and its gender dimorphrism still exsit 5 days after resuscitation.Therefore，We aim to determine the lung injury after T/HS and to compare its gender difference 5 days after resuscitation.

Methods

Male and female Sprague-Dawley rats were subjected to a midline laparotomy（trauma）and either hemorrhagic shock or sham shock.At the end of the shock period，the animals were resuscitated with Ringer lactate 3 times of their shed blood.Five days postresuscitation，the animals were sacrificed and lung permeability was measured.

Results

Lung permeability and pulmonary leukosequestration in T/HS animals were increased significantly 5 days.Females subjected to T/HS had increased pulmonary permeability，increased BALF protein/plasma protein ratio and increased pulmonary myeloperoxidase levels than males.Males gained while females lost body weight during study period.

Conclusion

Lung injury still can be seen 5 days aftar resuscitation.The injury is more severe in female than in male.