目的

探讨健脾化瘀中药对肝癌转移细胞凋亡的影响。

方法

制作裸鼠人肝癌MHCC97裸鼠模型，观察健脾化瘀中药对高、低转移中药组，高、低转移对照组，空白对照组转移灶的形成、瘤重、瘤体大小、肝内外转移、细胞凋亡峰百分率之间影响的差异。

结果

所有高、低转移组均全部能够成瘤，健脾化瘀中药抑制肝癌的肝内转移不明显，而能够有效地抑制肝癌的肝外转移，尤其对高转移细胞株的肝外转移抑制更明显,所有的组别都未见肝癌肺内转移，但肠道、脾脏明显发生转移，高转移中药组与高转移对照组之间、低转移中药组与低转移对照组之间差异有统计学意义（P＜0.05）。高转移对照组细胞凋亡百分率为（9.62±2.44）%，明显高于高转移中药组的（4.20±1.01）%，两组间比较差异有显著性意义（P＜0.01）；而低转移对照组细胞凋亡百分率为（16.52±3.11）%，高于低转移中药组的（11.72±2.44）%，两组间比较差异有统计学意义（P＜0.05）。

结论

健脾化瘀中药诱导人肝癌MHCC97高、低转移细胞株凋亡，对高转移细胞株作用尤为明显，抑制高、低转移细胞株的远处转移，但对肝脏成瘤影响不大。

Objective

To investigate the apoptosis of metastasis in hepatocellular carcinoma by Traditional Chinese Medicine-JianPiHuaYu (TCMJ).

Methods

Metastatic models of human hepatocellular carcinoma in thirty-fine nude mice (MHCC-97)were made and randomly divided into five groups: Chinese herbal medicine of high metastatic group and its control, the Chinese herbal medicine of low metastatic group and its control, the blank control group. The appearance, size, weigh, and metastasis in and outside liver,the rate of apoptosis peak were observed.

Results

Metastasis appeared in the entire study group. TCMJ couldn’t stop the formation of cancer inside liver but it could stop the metastasis beyond the liver effectively,especially in high group.Furthermore,there were differences in the rate of apoptosis peak,weigh,size,and metastasis beyond liver and the high metastasis group benefit more than the lower metastasis group. tumors were found in the gastrointestinal and spleen,but not lung.

Conclusion

TCMJ can induce apoptosis of high and low metastatic cells of HCC(MHCC-97), especially in high metastatic cells line.It also inhibits distant metastasis in lower and high metastasis cell line,but can not prevent the HCC from relapsing.