目的

探讨内皮素受体拮抗剂（ET-RA）对重症急性胰腺炎（SAP）大鼠心肌损伤的治疗作用。

方法

将30 只SD 大鼠随机分成3 组：重症急性胰腺炎组（SAPG）：胰管逆行注射5%牛黄胆酸钠的方法建立大鼠SAP 模型；正常对照（CG）组：处理方法同上，改推注同体积的生理盐水；ET-1 受体拮抗剂治疗组（ET-RAG）：SAP 成模后6h 腹腔内注射ET-RA，对照组与SAPG 注射等体积生理盐水。观察24 h 后取标本，分别测定各组血浆淀粉酶（AML）、激酸激酶（CK）及其同工酶（CK-MB）、乳酸脱氢酶-1（LDH1）、ET-1、TNF-α 的变化。

结果

AML、CK-MB、CK、LDH-1、ET-1、TNF-α 在SAPG 及ET-RAG 均显著高于正常对照组（P＜0.01），而ET-RAG 中以上指标的浓度亦显著低于SAPG（P＜0.05）。

结论

ET-RA 对SAP 时的心肌损伤有较好的治疗作用。

Objective

To explore the effect of treatment of endothelin receptor antagonist (ET-RA) on heart injury in severe acute pancreatitis(SAP) rats.

Methods

Thirty SD rats were randomly allocated into 3 groups: SAP group (SAPG): Main pancreatic duct was perfused with Sodium Cholate(5%)to induce SAP;Control group (CG):To do the same as that of SAP group,except with normal saline;ET-RA group (ET-RAG):Rats were treated with intraperitoneal injection of ET-RA after the models of SAP were done for 6 hours, while rats of the other two groups were treated with intraperitoneal injection of normal saline.Serum AML,creatine kinase(CK),CK-MB,lactate dehydrogenase 1(LDH1),ET-1 and TNF-α were measured after the models of SAP were done for 24 h.

Results

The levels of serum AML, CK, CK-MB, LDH1, ET-1 and TNF-α in SAPG and ET-RAG were significantly increased as compared with CG(P＜0.01).The levels of all indexes in ET-RAG were decreased than that in SAPG(P＜0.05).

Conclusion

ET-RA have certain effect of treatment on heart injury in SAP rats.