目的

研究门静脉高压症（portal hypertension,PH）脾亢脾与正常脾组织细胞因子的表达差异，为进一步探讨PH脾亢的发生机制和脾亢脾的免疫功能奠定基础。

方法

正常脾组织取自脾外伤手术切除的脾脏（A组，6例），脾亢脾组织取自PH脾亢患者手术切除的脾脏（B组，14例）；无菌取材后提取两组脾组织的蛋白质，用RayBio Human Cytokine Array 5.1细胞因子芯片（美国Ray生物技术公司）同时检测两组脾组织79个细胞因子的表达，得到两组细胞因子表达点阵图，用ScanAlyze图像分析软件对两组间的细胞因子表达差异进行统计学分析。

结果

79个细胞因子中，共有26个在两组间的表达呈现明显差异。在PH脾亢脾组织中表达上调的细胞因子共有21个，其中包括与单核细胞趋化和巨噬细胞活化相关的细胞因子如MCSF、TNF-β、IFN-γ、IL-10和SDF-1/CXCL12等，以及与血管新生、纤维化和组织改建相关的细胞因子如VEGF、TGF-β1、MIF、FGF-9、Flt-3、Ligand和IGFBP-2等。脾亢脾组织表达下调的细胞因子共有5个。

结论

PH脾亢脾与正常脾组织细胞因子存在明显表达差异，这些差异表达的细胞因子可能是导致PH脾脏的免疫功能变化及脾亢发生的重要因素之一，但尚需进一步研究证实。

Objective

To study the differential expression of cytokines between portal hypertensive(PH) hypersplenic tissue and normal splenic tissue, then to analyze the significance of differential expression and provide clues for deep research of the mechanisms of hypersplenism and the immunologic changes of hypersplenic spleen.

Methods

The normal splenic tissue specimens from surgical therapy for traumatic splenic rupture of healthy adults(group A)and the hypersplenic tissue specimens from surgical therapy for hypersplenism due to PH patients (group B) were collected aseptically. Extract proteins and quantify protein concentration by using BCA method.Then the RayBio Human Cytokine Array 5.1(Ray Biotech corp.USA)was used to detect the differential expression of 79 cytokines between PH hypersplenic tissue and normal splenic tissue to construct a differential expression profile,and the ScanAlyze software was used for statistical analysis.

Results

Among 79 cytokines detected,there were 26 cytokines up-regulate significantly in hypersplenic tissue compared with normal splenic tissue,most of which were cytokines associated with monocytes chemotaxis and macrophages activation,such as MCSF,TNF-β, IFN-γ, IL-10 and SDF-1/CXCL12. Further more, cytokines associated with angiogenesis, fibrosis and tissue remodeling up-regulate in PH hypersplenic tissue as well, such as VEGF, TGF-β1, MIF, FGF-9,Flt-3 Ligand and IGFBP-2. And there were 5 cytokines down-regulate in PH hypersplenic tissue.

Conclusion

There are significant differential expression profile between PH hypersplenic tissue and normal splenic tissue. The differential expression of cytokines will be associated with the immunological status of hypersplenic spleen and the developmentof hypersplenism.But the function need for deeper research.