目的

研究热休克蛋白90α在人胃癌组织和正常胃黏膜组织中的表达，并探讨其表达与胃癌的发生、发展及生物学行为的关系。

方法

收集临床46例胃癌组织和相应的正常胃黏膜组织标本，应用SP免疫组化法检测HSP90α的表达情况，结合患者的临床病理资料分析检测结果。

结果

HSP90α在胃癌组织中的阳性表达率为82.6%，明显高于正常胃黏膜组织中的表达（21.7%）；胃癌组织中HSP90α阳性表达率与患者年龄、性别、肿瘤大小及分化程度等无明显相关性。HSP90α在肿瘤浸润至肌层或越过肌层者的阳性表达率（89.5%）明显高于肿瘤局限于黏膜或黏膜下层者（50.0%）。淋巴结转移7个以上（即N2、N3期）的病例中HSP90α阳性表达率明显高于淋巴结转移为N0和N1的病例（96.2%vs 65.0%，P＜0.05）；TNM分期Ⅲ、Ⅳ期阳性表达率也要高于Ⅰ、Ⅱ期（92.9% vs 66.7%，P＜0.05）。

结论

HSP90α在胃癌组织中呈现高表达，并且在浸润深的肿瘤或淋巴结转移多和越晚期的胃癌组织中表达越高。提示HSP90α可作为胃癌预后判定监测指标。

Objective

To estimate the expression of heat-shock pr otein 90α(HSP90α)in gastric cancer and normal gastric mucosa,and to find out its relationships to the occurring,developing and biological behavior of gastric cancer.

Methods

The specimens of 46 cases with gastric cancer were collected,including 46 cases of gastric cancer and their normal mucosa. Immunohistochemical staining was performed for the expressions of HSP90α,and the results were analyzed with patients'clinicopathological data.

Results

The positive expression rates of HSP90αwas 82.6%,which was higher than that in normal mucosa (21.7%).The expression of HSP90α in gastric cancer was not closely relative to age,sex,tumor size and different differentiation extent.The positive ex pression rate of HSP90αin muscularis/over-muscularis group was higher than that in mucosa/submucosa group(89.5%vs 50.0%,P＜0.05).The positive expression rate of HSP90α in gastric cancer in N2,N3 stage was higher than that in gastric cancer in N0 and N1 stage (96.2% vs 65.0%, P＜0.05). The positive expression rate of HSP90α in TNM Ⅲ,Ⅳstage was 92.9%,higher than that in other stages (66.7%,P＜0.05).

Conclusions

The level of HSP90α expression in gastric cancer is higher than that in normal gastric mucosa.With more lymph nodes metastasized,HSP90α expression increases.The more advanced the gastric cancer,the more the HSP90αexpression.The high expression of HSP90α in gastric cancer indicates poor prognosis.