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中华普通外科学文献(电子版) ›› 2010, Vol. 04 ›› Issue (02) : 100 -104. doi: 10.3877/cma.j.issn.1674-0793.2010.02.004

所属专题: 文献

论著

去神经支配对大鼠创面愈合的影响
舒斌1, 祁少海1,(), 黄勇1, 赖文2, 毛任翔1, 谢举临1, 徐盈斌1, 刘旭盛1, 李霞3   
  1. 1. 510080 广州,中山大学附属第一医院烧伤外科
    2. 广东省人民医院烧伤科
    3. 山东鲁抗医药股份有限公司
  • 收稿日期:2009-12-12 出版日期:2010-04-01
  • 通信作者: 祁少海
  • 基金资助:
    广东省科技计划项目基金资助(2007B031002006); 创伤、烧伤与复合伤国家重点实验室开放基金(SKLKF 200808); 广东省自然科学基金(8451008901000894)

Effect of denervation on skin wound healing in rats

Bin SHU1, Shao-hai QI1,(), Yong HUANG1, Wen LAI2, Ren-xiang MAO1, Ju-lin XIE1, Ying-bin XU1, Xu-sheng LIU1, Xia LI3   

  1. 1. Department of Burns, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
  • Received:2009-12-12 Published:2010-04-01
  • Corresponding author: Shao-hai QI
  • About author:
    Corresponding author: QI Shao-hai, Email:
引用本文:

舒斌, 祁少海, 黄勇, 赖文, 毛任翔, 谢举临, 徐盈斌, 刘旭盛, 李霞. 去神经支配对大鼠创面愈合的影响[J/OL]. 中华普通外科学文献(电子版), 2010, 04(02): 100-104.

Bin SHU, Shao-hai QI, Yong HUANG, Wen LAI, Ren-xiang MAO, Ju-lin XIE, Ying-bin XU, Xu-sheng LIU, Xia LI. Effect of denervation on skin wound healing in rats[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2010, 04(02): 100-104.

目的

通过制备去神经支配大鼠创面模型,观察去神经大鼠创面愈合率、创面血管和皮肤增殖改变情况,为去神经创面愈合机制提供理论依据。

方法

分离并切断SD大鼠胸9~腰1右侧脊神经,造模后在背部对称制作两个直径为1.0 cm的圆形创面,分别观察两侧创面愈合时间、创面大体和组织形态学、创面细胞增殖活性、创面修复过程中血管再生情况,并对数据进行统计学分析。

结果

实验组愈合时间在4周,对照组为3周;实验组创面上皮化速度慢于对照组;实验组愈合表皮层较薄,上皮细胞大小不一;愈合时真皮和表皮层细胞增殖活跃,2~4周时实验组增殖活性低于对照组(P<0.05);创面微血管主要分布于真皮层,密度随愈合时间增加,但2~4周实验组表现较对照组弱(P<0.05)。

结论

去神经后皮肤创面细胞增殖低下,愈合时间延长,血管再生现象受到抑制。

Objective

To observe the effect of denervation on wound healing rate, cell proliferation activity and vascular regeneration.

Methods

Right spinal nerves of T9 to L1 of were isolated and dissected on forty Spraque-Dawley(SD) rats. Subsequently, 1.0cm-diameter round full-thickness cutaneous wounds were made symmetrically within the denervated area on the right side of the back(experimental group) and the normal innervated area on the left side(control group). Wounds were measured and harvested at 1、2、3、4 weeks. Wound healing rate, histology and morphometry, cell proliferation activity and vascular regeneration were analyzed over time.

Results

The wounds of control group and experimental group were completely heal on 3rd and 4th week respectively. Epithelization of control group appeared earlier and faster than experimental group, wound healing rate of experimental group were decreased significantly at 2nd and 3rd week (P<0.05) as well. The cell proliferation activity of wounds surface were higher in dermis and epidermis. Furthermore, cell proliferation activity of experimental group were weaker than control group during 2 to 4 weeks (P<0.05) . Regenerated wound capillary vessel laied in dermis mainly. Wound capillary vessel density was lower significantly in the experimental group (P<0.05) at 2 to 4 weeks when compared with the controls.

Conclusion

Loss of innervation on skin may be responsible for the cell proliferation activity hypoproliferation, wound healing delay and vascular regeneration depression.

图1 单侧去神经造模
图2 单侧去神经造模后制创
表1 制创后不同时间点两组创面愈合率的比较(%,±s
图3 4周时实验组表皮层较薄,细胞层次欠清,表皮细胞体积大小不一(正置相差显微镜×100)
图4 4周时对照组表皮层较厚,可达7~8层,表皮细胞体积较大(正置相差显微镜×100)
图5 4周时实验组PCNA表达情况(正置相差显微镜×200)
图6 4周时对照组PCNA表达情况(正置相差显微镜×200)
表2 制创后不同时间点两组创面PCNA表达的比较(%,±s
图7 2周时实验组Ⅷ因子表达情况(正置相差显微镜×100)
图8 2周时对照组Ⅷ因子表达情况(正置相差显微镜×100)
图9 4周时实验组Ⅷ因子表达情况(正置相差显微镜×100)
图10 4周时对照组Ⅷ因子表达情况(正置相差显微镜×100)
表3 制创后不同时间点两组创面Ⅶ因子表达的比较(个/视野,±s
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