观察吡咯烷二硫代氨基甲酸盐（PDTC）对高脂饮食模型大鼠主动脉内皮细胞的影响并探讨其机制。

SD大鼠18只随机分为高脂对照组、PDTC干预高脂组、空白对照组。12周后取主动脉行电镜检查，以电泳迁移率变动分析法（EMSA）检测核因子κB（NF-κB）活性，放射免疫法测定血清肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）水平，酶联免疫吸附实验测量可溶性血栓调节蛋白（sTM）水平。

高脂对照组的血管内皮细胞存在明显的崩解现象，而PDTC干预组损伤轻微。PDTC干预高脂组sTM水平显著高于空白对照组，低于高脂对照组（P<0.05）。与空白对照组相比，高脂对照组主动脉NF-κB活性、血清TNF-α和IL-6水平显著升高，PDTC干预高脂组也有升高但明显低于高脂对照组（P<0.05）。NF-κB活性与TNF-α、IL-6、sTM水平呈正相关（P<0.05）。

PDTC可以通过抑制高脂饮食模型大鼠的主动脉中NF-κB的活化、减轻过度的炎症反应而发挥保护内皮细胞的作用。

To examine the effect of pyrrolidine dithiocarbamate (PDTC) on the aorta endothelial cells in rats with high lipid diet.

Eighteen Sprague Dawley rats were randomly divided into three groups: high lipids group, PDTC and high lipid group(PDTC group) and normal control group. The aortas were harvested for histomorphometry and transmission electron microscope 12 weeks after the induction of high plasma lipid. The nuclear factor κB (NF-κB) activity was measured by use of electrophoretic mobility shift assays (EMSA) in aortas. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in serum were measured by use of radio-immunity and the level of soluble thrombomodulin (sTM) in serum was measured by enzyme linked immunosorbent assay (ELISA).

There was obvious necrosis or apoptosis for aorta endothelial cells in high lipids group, but this phenomenon was not clear in PDTC group. The level of sTM in the PDTC group was significantly higher than that of normal control group and lower than that of high lipids group (P<0.05). The activity of NF-κB and the levels of IL-6, TNF-α were evidently higher in high lipids group than normal control group and PDTC group. There was a positive relation between the NF-κB activity and the levels of TNF-α, IL-6 and sTM (P<0.05).

PDTC can protect aorta endothelial cells by inhibiting the activity of NF-κB and the inflammatory response in rats with high lipid diet.