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中华普通外科学文献(电子版) ›› 2010, Vol. 04 ›› Issue (06) : 553 -556. doi: 10.3877/cma.j.issn.1674-0793.2010.06.012

所属专题: 文献

论著

血清磷脂酰肌醇蛋白聚糖3蛋白测定在肝细胞癌诊断中的价值
付顺军1, 李绍强1,(), 林杰1, 彭宝岗1, 汪谦1, 梁力建1   
  1. 1. 510080 广州,中山大学附属第一医院肝胆外科
  • 收稿日期:2010-06-23 出版日期:2010-12-01
  • 通信作者: 李绍强
  • 基金资助:
    广东省自然科学基金(07001626); 广东省科技计划项目(2009B030801124); 广东省科学医学基金(A2007172)

Diagnostic value of serum Glypican-3 for hepatocellular carcinoma

Shun-jun FU1, Shao-qiang LI1,(), Jie LIN1, Bao-gang PENG1, Qian WANG1, Li-jian LIANG1   

  1. 1. Department of Hepatobiliary Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
  • Received:2010-06-23 Published:2010-12-01
  • Corresponding author: Shao-qiang LI
  • About author:
    Corresponding author: LI Shao-qiang, Email:
引用本文:

付顺军, 李绍强, 林杰, 彭宝岗, 汪谦, 梁力建. 血清磷脂酰肌醇蛋白聚糖3蛋白测定在肝细胞癌诊断中的价值[J/OL]. 中华普通外科学文献(电子版), 2010, 04(06): 553-556.

Shun-jun FU, Shao-qiang LI, Jie LIN, Bao-gang PENG, Qian WANG, Li-jian LIANG. Diagnostic value of serum Glypican-3 for hepatocellular carcinoma[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2010, 04(06): 553-556.

目的

检测血清中磷脂酰肌醇蛋白聚糖3(Glypican-3,GPC3)的浓度,探讨其对原发性肝细胞癌(HCC)的诊断价值。

方法

用酶链免疫吸附试验(ELISA)对67例HCC患者、41例肝硬化患者、30例健康者血清中GPC3进行测定,化学发光法检测甲胎蛋白(AFP)。

结果

HCC患者血清GPC3平均浓度为(10.85±0.58)μg/L;肝硬化患者血清GPC3平均浓度为(2.45±0.61)μg/L;健康者血清GPC3平均浓度为(1.14±0.22)μg/L。HCC患者血清GPC3浓度明显高于肝硬化患者和健康者(H=327.000,P=0.0001),肝硬化患者与健康者差异无统计学意义(Z=0.792,P=0.429)。当以3 μg/L为诊断界值时,GPC3对HCC的敏感性和特异性分别为88.1%和85.9%。当AFP以20 μg/L为诊断界值时,其对HCC的敏感性和特异性分别为65.7%和76.2%。GPC3对HCC的敏感性显著高于AFP(χ2=10.995,P=0.001)。在23例AFP阴性的HCC患者中有17例GPC3阳性,阳性率为73.9%(17/23)。GPC3的ROC曲线下面积大于AFP(0.898 vs 0.814)。

结论

血清GPC3对HCC有很高的敏感性和特异性,可作为一种新的肿瘤标志物用于HCC的诊断。

Objective

To detect the concentration of Glypican-3(GPC3) protein in serum and to explore its diagnostic value for hepatocellular carcinoma(HCC).

Methods

The concentration of serum GPC3 in 67 HCC patients, 41 patients with liver cirrhosis and 30 healthy subjects controls were detected by Enzyme-linked immunos-orbent assay(ELISA). Serum alpha-fetoprotein(AFP) level was detected by chemilum-inesence immunoassay.

Results

The mean concentration of serum GPC3 was (10.85±0.58)μg/L, (2.45±0.61)μg/L and (1.14±0.22)μg/L in HCC patients, liver cirrhosis patients and healthy subjects. The concentration of serum GPC3 protein in HCC patients was markly higher than in liver cirrhosis patients and healthy subjects(H=327.000,P=0.0001), but there was comparable between liver cirrhosis patients and healthy subjects (Z=0.792, P=0.429). At a cut off value of 3μg/L, the diagnostic sensitivity and specifity of GPC3 for HCC were 88.1% and 85.9%, respectively. And at a cut off value of 20 μg/L, the diagnostic sensitivity and specifity of AFP for HCC were 65.7% and 76.2%, respectively. The sensitivity of GPC3 was significantly higher than that of AFP (χ2=10.995, P=0.001). In the 23 HCC patients whose AFP<20 μg/L, the positive rate of GPC3 protein was 73.9% (17/23). The surface area under ROC curve of GPC3 was larger than that of AFP(0.898 vs 0.814).

Conclusions

Serum GPC3 is highly sensitive and specific for detecting HCC. It can be used as a new marker for the diagnosis of HCC.

图1 GPC3血清浓度标准曲线
表1 HCC、肝硬化患者和健康者血清GPC3、AFP测定结果(μg/L,±s
图2 HCC、肝硬化患者及健康者血清GPC3和AFP浓度的ROC曲线
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