小鼠肝脏应用解剖及标准肝部分切除模型的建立

doi:10.3877/cma.j.issn.1674-0793.2014.02.005

中华普通外科学文献(电子版) ›› 2014, Vol. 08 ›› Issue (02) : 102 -105. doi: 10.3877/cma.j.issn.1674-0793.2014.02.005

所属专题：文献；

论著

小鼠肝脏应用解剖及标准肝部分切除模型的建立

周健¹, 郭志勇¹, 鞠卫强¹, 焦兴元¹, 王小平¹, 韩明¹, 胡安斌¹, 巫林伟¹, 袁小鹏¹, 马毅¹, 朱晓峰¹, 何晓顺¹^,^†(

)

^1. 510080　广州，中山大学附属第一医院器官移植中心

收稿日期:2013-01-16 出版日期:2014-04-01
通信作者: 何晓顺

Application anatomy of the liver and establishment of standard partial hepatectomy model in mice

Jian Zhou¹, Zhiyong Guo¹, Weiqiang Ju¹, Xingyuan Jiao¹, Xiaoping Wang¹, Ming Han¹, Anbin Hu¹, Linwei Wu¹, Xiaopeng Yuan¹, Yi Ma¹, Xiaofeng Zhu¹, Xiaoshun He¹^,^†()

^1. Organ Transplantation Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China

Received:2013-01-16 Published:2014-04-01
Corresponding author: Xiaoshun He
About author:
Corresponding author: He Xiaoshun, Email: gdtrc@163.com

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目的

观测C57BL/6J小鼠肝脏的解剖及建立70%、90%标准肝部分切除模型。

方法

60只成年SPF级C57BL/6J小鼠随机分3组。A组小鼠称量体质量、全肝质量及各肝叶质量，计算全肝质量/体质量比、70%和90%肝比例。采用直视下分步单纯丝线结扎肝脏解剖功能单位后再切除肝叶的方法，B组行70%肝切除；C组行90%肝切除。观察术后动物生存情况。

结果

小鼠全肝质量为（1.325±0.029）g，体质量为（26.5±1.14）g，左外侧叶质量为（0.433±0.041）g，中叶为（0.411±0.035）g，右前叶为（0.140±0.011）g，右后叶为（0.175±0.015）g，尾状叶为（0.099±0.006）g，全肝质量/体质量比为（4.92±0.13）%。肝左外侧叶与中叶之和约占全肝比重的70%；仅剩余尾状叶则切除肝比重约为90%。B组和C组手术成功率为100%，B组术后1周动物存活率为100%，C组术后1 d存活率为30%（6/20），2 d存活率为0。

结论

本研究首次明确小鼠各肝叶比例。采用直视下分步单纯丝线结扎肝脏解剖功能单位后再切除肝叶的方法，极易建立稳定、可靠的小鼠70%和90%标准肝部分切除模型。

关键词: 部分肝切除, 应用解剖, 小鼠, 模型, 标准

Objective

To observe anatomy of liver and establish 70% and 90% standard partial hepatectomy model in C57BL/6J mice.

Methods

Sixty adult mice with a C57BL/6J SPF stage were randomly equably divided into three groups, group A, group B and group C. Weight of body, total liver, and every single liver lobe were weighed in group A, ratio of total liver weight/body weight, and proportion of 70% and 90% liver was calculated, respectively. 70% partial hepatectomy in group B and 90% partial hepatectomy in group C were performed, using step by step visually ligaturing anatomic functional unit of liver by only threads.

Results

The weight of total liver, body, left lateral lobe, median lobe, right anterior lobe, right posterior lobe and caudate lobe in mice were (1.325±0.029) g, (26.500±1.140) g, (0.433±0.041) g, (0.411±0.035) g, (0.140±0.011) g, (0.175±0.015) g, (0.099±0.006) g, respectively, the ratio of total liver weight/body weight was (4.92±0.13)%, the weight of left lateral lobe and median lobe accounted for 70% of the whole liver, and the weight of other parts except caudate lobe preserved was 90%. The successful operation rate in both group B and C was 100%. The survival rate was 100% one week after operation in group B, 30% (6/20) one day after operation in group C, respectively, and no survival in group C two days after operation.

Conclusions

This study ascertains the proportion of every liver lobe in mice for the first time. Using step by step visually ligaturing anatomic functional unit of liver by only threads, it is easy to establish stable and credible 70% and 90% partial hepatectomy model in mice.

Key words: Partial hepatectomy, Application anatomy, Mice, Model, Standard

图1 小鼠肝脏解剖示意图和丝线结扎位置及顺序

表1 20只A组小鼠的肝叶质量及占全肝体质量的比例

部位	体质量（g）	全肝比例（%）
全肝	1.325±0.029	100.00±0.00
左外侧叶	0.433±0.041	32.72±3.04
中叶	0.411±0.035	31.07±2.95
右前叶	0.140±0.011	10.60±0.85
右后叶	0.175±0.015	13.23±1.14
尾状叶	0.099±0.006	7.44±0.52

表1 20只A组小鼠的肝叶质量及占全肝体质量的比例

部位	体质量（g）	全肝比例（%）
全肝	1.325±0.029	100.00±0.00
左外侧叶	0.433±0.041	32.72±3.04
中叶	0.411±0.035	31.07±2.95
右前叶	0.140±0.011	10.60±0.85
右后叶	0.175±0.015	13.23±1.14
尾状叶	0.099±0.006	7.44±0.52

图2 小鼠肝脏离体解剖分叶及肝叶比例观察

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