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中华普通外科学文献(电子版) ›› 2014, Vol. 08 ›› Issue (02) : 116 -120. doi: 10.3877/cma.j.issn.1674-0793.2014.02.008

所属专题: 文献

论著

免疫性血小板减少症脾切除疗效与外周血Th亚群细胞因子表达的关系
郑冬1, 张珑涓2, 汪谦3, 王俊3, 黄晨松3, 郑朝旭3,()   
  1. 1. 510080 广州,中山大学附属第一医院血液内科
    2. 510080 广州,中山大学附属第一医院外科实验室
    3. 510080 广州,中山大学附属第一医院普通外科
  • 收稿日期:2013-02-16 出版日期:2014-04-01
  • 通信作者: 郑朝旭
  • 基金资助:
    广东省科技计划项目(2010B031600212,2012B031800284)

Relationship between the hematological outcomes of splenectomy and the expression of Th cytokines of peripheral blood in ITP patients

Dong Zheng1, Longjuan Zhang2, Qian Wang3, Jun Wang3, Chensong Huang3, Chaoxu Zheng3,()   

  1. 1. Department of Hematology Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
  • Received:2013-02-16 Published:2014-04-01
  • Corresponding author: Chaoxu Zheng
  • About author:
    Corresponding author: Zheng Chaoxu, Email:
引用本文:

郑冬, 张珑涓, 汪谦, 王俊, 黄晨松, 郑朝旭. 免疫性血小板减少症脾切除疗效与外周血Th亚群细胞因子表达的关系[J/OL]. 中华普通外科学文献(电子版), 2014, 08(02): 116-120.

Dong Zheng, Longjuan Zhang, Qian Wang, Jun Wang, Chensong Huang, Chaoxu Zheng. Relationship between the hematological outcomes of splenectomy and the expression of Th cytokines of peripheral blood in ITP patients[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2014, 08(02): 116-120.

目的

探讨原发性免疫性血小板减少症(ITP)脾切除血液学疗效与外周血辅助性T细胞(Th)亚群细胞因子的mRNA表达水平的关系。

方法

采用QuantiGene Plex(QGP)方法,分别检测14例手术有效(R组)和8例手术无效(NR组)的ITP患者腹腔镜脾切除术前后外周血Th1(IL-2、IFN-γ)、Th2(IL-4、IL-5、IL-6、IL-10)、Th3(TGF-β1)、Th17(IL-17)细胞因子的mRNA表达水平的变化。对照组为30例健康体检者。

结果

术前及术后R组TGF-β1 mRNA的表达水平均明显高于NR组(P值分别为0.001和0.006)及对照组(P值分别为0.001和<0.001)。术前及术后R组和NR组之间IL-2、IFN-γ与IL-17 mRNA的表达水平差异均无统计学意义。

结论

脾切除疗效不同的ITP患者TGF-β1的mRNA表达水平存在差异,检测术前TGF-β1的表达水平有助于预测手术疗效。

Objective

To investigate the relationship between the hematological outcomes of splenectomy and the mRNA expressions of Th cytokines in primary immune thrombocytopenia (ITP) patients.

Methods

The mRNA expression of Th1 (IL-2, IFN-γ), Th2 (IL-4, IL-5, IL-6, IL-10), Th3 (TGF-β1), and Th17 (IL-17) cytokines in peripheral blood before and after laparoscopic splenectomy were detected by the QuantiGene Plex method from fourteen cases of responder group (Group R) and eight cases of non-responder group (Group NR). Thirty healthy subjects were served as the control group.

Results

TGF-β1 mRNA expressions before and after splenectomy in Group R were significantly higher than those in Group NR (P values were 0.001 and 0.006, respectively) and control group (P values were 0.001 and <0.001, respectively). No significant differences of IL-2, IFN-γ and IL-17 mRNA expression before or after splenectomy between Group R and Group NR were observed.

Conclusions

There are different TGF-β1 mRNA expressions in ITP patients with different responses to splenectomy. Preoperative detection of TGF-β1 expression is helpful to predict the outcome of surgery.

表1 目的基因和管家基因探针组信息表
表2 ITP患者和健康者一般资料的分组比较(±s)
表3 术前R组与NR组细胞因子的比较(±s)
表4 术后R组与NR组细胞因子的比较(±s)
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