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中华普通外科学文献(电子版) ›› 2021, Vol. 15 ›› Issue (01) : 18 -22. doi: 10.3877/cma.j.issn.1674-0793.2021.01.004

所属专题: 文献

论著

垂体肿瘤转化因子1和Ten-eleven转运因子1在肝细胞癌中的表达及相关性分析
李庆贺1,(), 张家耀1, 樊斌1, 王宇2   
  1. 1. 445000 恩施土家族苗族自治州中心医院肝胆外科
    2. 430077 武汉大学中南医院肝胆外科
  • 收稿日期:2020-02-27 出版日期:2021-02-01
  • 通信作者: 李庆贺
  • 基金资助:
    湖北省卫生计生西医类一般项目(WJ2015MB032)

Expression of PTTG1 and TET1 in hepatocarcinoma tissues and its relationship with clinico-pathological parameters

Qinghe Li1,(), Jiayao Zhang1, Bin Fan1, Yu Wang2   

  1. 1. Department of Hepatobiliary Surgery, Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, China
    2. Department of Hepatobiliary Surgery, Central South Hospital of Wuhan University, Wuhan 430077, China
  • Received:2020-02-27 Published:2021-02-01
  • Corresponding author: Qinghe Li
引用本文:

李庆贺, 张家耀, 樊斌, 王宇. 垂体肿瘤转化因子1和Ten-eleven转运因子1在肝细胞癌中的表达及相关性分析[J/OL]. 中华普通外科学文献(电子版), 2021, 15(01): 18-22.

Qinghe Li, Jiayao Zhang, Bin Fan, Yu Wang. Expression of PTTG1 and TET1 in hepatocarcinoma tissues and its relationship with clinico-pathological parameters[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(01): 18-22.

目的

探讨肝癌组织中垂体肿瘤转化因子1(PTTG1)及Ten-eleven转运因子1(TET1)表达水平及其与临床病理的相关性,为预测肝癌的发生及发展提供理论基础。

方法

选择2017年2月至2019年1月恩施土家族苗族自治州中心医院行根治性切除手术肝细胞癌病例标本129例作为实验组,另选取129例对应的癌旁组织作为对照组。应用免疫组织化学法检测PTTG1和TET1在肝癌组织及癌旁组织中的表达,分析PTTG1和TET1的表达与肝细胞癌临床病理指标的关系,对PTTG1和TET1表达水平进行相关性分析。

结果

PTTG1在肝细胞癌组织的高表达率(53.5%,69/129)显著高于癌旁组织(1.6%,2/129)(χ2=84.646,P<0.001),TET1在癌组织的表达(45.0%,58/129)显著低于癌旁组织(81.4%,105/129)(χ2=35.255,P<0.001)。PTTG1的表达水平与TET1表达水平呈负相关关系(r=-0.726,P<0.001)。PTTG1的表达水平与卫星结节、Edmondson病理分级密切相关(P<0.05),TET1的表达水平与肿瘤包膜状况、卫星结节密切相关(P<0.05)。

结论

PTTG1和TET1在肝细胞癌组织中异常表达,并且与肝细胞癌的发生、发展密切相关,临床上有望成为肝癌的诊断和根治性切除治疗预后的生物标志物。

Objective

To investigate the correlation between the expression levels of pituitary tumor transforming gene 1 (PTTG1) and ten-eleven translocation 1 (TET1), and the clinicopathological parameters in liver cancer tissues and paracancer tissues, and to provide theoretical basis for the prediction of the occurrence and development of liver cancer.

Methods

From February 2017 to January 2019, 129 cases of hepatocellular carcinoma specimens undergoing radical resection in Central Hospital of Enshi Tujia and Miao Autonomous Prefecture were selected as the experimental group, and 129 corresponding adjacent tissues were selected as the control group. Immunohistochemistry technique was used to detect the expression of PTTG1 and TET1 in the two groups. The relationship between the clinicopathological parameters and the expression of PTTG1 and TET1 was analyzed. In addition, the relationship between the PTTG1 protein and TET1 protein was correlation analyzed.

Results

The high expression rate of PTTG1 in hepatocellular carinoma tissues (53.5%, 69/129) was significantly higher than that in adjacent tissues (1.6%, 2/129) (χ2=84.646, P<0.001). The expression of TET1 in hepatocellular carinoma tissues (45.0%, 58/129) was significantly lower than that in adjacent tissues (81.4%, 105/129) (χ2=35.255, P<0.001). The expression level of PTTG1 was negatively correlated with that of TET1 (r= -0.726, P<0.001). The expression level of PTTG1 was closely related to satellite nodules and Edmondson pathological grade (P<0.05), and the expression level of TET1 was closely related to the tumor envelope status and satellite nodules (P<0.05).

Conclusion

PTTG1 protein and TET1 protein are expressed abnormally in hepatocellular carcinoma tissues, and are closely correlated with the occurrence and development of hepatocellular carcinoma, which suggests PTTG1 protein and TET1 protein can be used as biological indicators for the diagnosis and prognosis of hepatocellular carcinoma.

表1 PTTG1、TET1蛋白表达与肝癌患者临床病理参数的关系[例(%)]
临床参数 例数 PTTG1蛋白 TET1蛋白
低表达 高表达 χ2 P 低表达 高表达 χ2 P
性别       0.254 0.614     0.607 0.436
  104 50(48.1) 54(51.9)     55(52.9) 49(47.1)    
  25 10(40.0) 15(60.0)     16(64.0) 9(36.0)    
年龄(岁)       0.009 0.925     0.009 0.922
  ≤40 65 30(46.2) 35(53.8)     35(53.8) 30(46.2)    
  > 40 64 30(46.9) 34(53.1)     36(56.3) 28(43.7)    
血清甲胎蛋白(μg/L)       0.369 0.544     <0.001 0.996
  ≤25 30 12(40.0) 18(60.0)     16(53.3) 14(46.7)    
  > 25 99 48(48.5) 51(51.5)     55(55.6) 44(44.4)    
乙肝表面抗原       1.486 0.223     <0.001 0.998
  阴性 10 5(50.0) 5(50.0)     5(50.0) 5(50.0)    
  阳性 119 55(46.2) 64(53.8)     66(55.5) 53(44.5)    
肝硬化       0.117 0.733     1.778 0.182
  无或轻度 89 40(44.9) 49(55.1)     45(50.6) 44(49.4)    
  中-重度 40 20(50.0) 20(50.0)     26(65.0) 14(35.0)    
ICGR15       0.031 0.861     0.811 0.368
  ≤10% 87 40(46.0) 47(44.0)     45(51.7) 42(48.3)    
  > 10% 42 20(47.6) 22(52.4)     26(61.9) 16(38.1)    
临床分期       0.025 0.873     0.010 0.921
  45 20(44.4) 25(55.6)     25(55.6) 20(44.4)    
  84 40(47.6) 44(52.4)     46(54.8) 38(45.2)    
肿瘤大小(cm)       0.661 0.416     0.518 0.472
  ≤5 50 26(52.0) 24(48.0)     30(60.0) 20(40.0)    
  > 5 79 34(43.0) 45(57.0)     41(51.9) 38(48.1)    
肿瘤包膜       0.025 0.873     46.020 <0.001
  完整 84 39(46.4) 45(53.6)     65(77.4) 19(22.6)    
  无或不完整 45 21(46.7) 24(53.3)     6(13.3) 39(86.7)    
卫星结节       27.952 <0.001     49.442 <0.001
  87 55(63.2) 32(36.8)     67(77.0) 20(23.0)    
  42 5(11.9) 37(88.1)     4(9.5) 38(90.5)    
Edmondson病理分级       30.132 <0.001     0.358 0.550
  Ⅰ~Ⅱ 83 54(65.1) 29(34.9)     48(57.8) 36(43.4)    
  Ⅲ~Ⅳ 46 6(13.0) 40(87.0)     23(50.0) 23(50.0)    
图1 垂体肿瘤转化因子1(PTTG1)及Ten-eleven转运因子1(TET1)表达水平的相关性
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