观察人粒细胞集落刺激因子（G-CSF）动员前后外周血细胞数量和标记的变化，评估G-CSF动员能力。

统计2004年3月至2018年12月期间中山市人民医院45例下肢缺血性疾病患者应用G-CSF动员前后外周血中的白细胞、中性粒细胞、单核细胞、红细胞和血小板数量和CD34+标记变化，形成配对资料。

动员前外周血中的白细胞、中性粒细胞、单核细胞、CD34+细胞绝对计数分别为（8.38±2.21）×10⁹/L、（5.26±2.33）×10⁹/L、（0.75±0.39）×10⁹/L、（9.55±19.35）个/μl，G-CSF动员第3天上升至（40.60±12.90）×10⁹/L、（34.88±11.74）×10⁹/L、（1.86±0.77）×10⁹/L、（33.45±47.42）个/μl，差异均有统计学意义（t=9.230、9.662、4.424、2.732，P=0.000、0.000、0.001、0.021）；单核细胞占比动员前为（8.15±1.67）%，动员第3天反降至（4.89±2.13）%，差异有统计学意义（χ2=-5.116，P<0.001）。停止动员后第3天外周血中的白细胞、中性粒细胞、单核细胞、CD34+细胞数量恢复到动员前水平。红细胞、血小板数量动员前后无明显变化，差异无统计学意义（t=1.428、0.538，P=0.184、0.602）。

G-CSF动员可以增加外周血中白细胞、中性粒细胞、单核细胞、CD34+细胞的数量，但动员单核细胞能力有限，而红细胞、血小板数量未受明显影响。

To assess human granulocyte colony stimulating factor (G-CSF) mobilization capacity through the changes of peripheral blood cell numbers and markers before and after the mobilization of G-CSF.

The number and CD34+ markers of leukocytes, neutrophils, monocytes, erythrocytes and platelet cells in peripheral blood cells of 45 patients with lower extremity ischemic diseases from March 2004 to December 2018 in Zhongshan City People’ s Hospital were analyzed before and after G-CSF mobilization.

The number of leukocytes, neutrophils, monocytes and CD34+ cells in peripheral blood before mobilization were (8.38±2.21)×10⁹/L, (5.26±2.33)×10⁹/L, (0.75±0.39)×10⁹/L, (9.55±19.35) μl, which increased to (40.60±12.90)×10⁹/L, (34.88±11.74)×10⁹/L, (1.86±0.77)×10⁹/L,(33.45±47.42) μl 3 days after mobilization, and the differences were statistically significant (t=9.230, 9.662, 4.424, 2.732, P=0.000, 0.000, 0.001, 0.021). Before mobilization, the proportion of monocytes was (8.15±1.67)%, 3 days after mobilization decreased to (4.89±2.13)%, with statistically significant difference (χ²=-5.116, P<0.001). Leukocytes, neutrophils, monocytes, and CD34+ cells in peripheral blood recovered to pre-mobilization levels on the third day after cessation of mobilization. There were no statistically significant differences in the number of erythrocytes and platelet cells before and after mobilization (t=1.428, 0.538, P=0.184, 0.602).

G-CSF can mobilize and increase the number of white blood cells, neutrophils, monocytes, CD34+ cells in peripheral blood, but the ability of mobilizing monocytes is limited, while erythrocytes and platelet cells are not affected.