增味小承气汤对梗阻性黄疸大鼠胃肠道动力的影响

doi:10.3877/cma.j.issn.1674-0793.2023.04.006

目的

探讨梗阻性黄疸（OJ）大鼠应用增味小承气汤后对肠道动力及血清胃动素、生长抑素质量浓度的影响。

方法

将SD雄性大鼠随机分为正常组、假手术组、手术建模梗阻性黄疸+中药组（OJ+M组）和手术建模梗阻性黄疸组（OJ组），每组10只。建立模型并进行实验干预，第3、5、7天自尾静脉取血，酶联免疫吸附实验（ELISA）法测定大鼠血清胃动素、生长抑素水平，术后第8天行活性炭肠推进实验，比较肠道动力变化。

结果

术后第5、7天，OJ+M组血清胃动素水平高于其他三组，组间差异有统计学意义（P<0.05）。胆管结扎术后第3天，OJ+M组大鼠血清生长抑素水平较假手术组高，但比正常组及OJ组低（P<0.05）；术后第5、7天，OJ+M组大鼠血清生长抑素水平显著低于其他三组（P<0.05）。正常组肠推进指数0.596±0.008，假手术组为0.470±0.021，OJ+M组为0.520±0.013，OJ组为0.350±0.009，OJ+M组高于假手术组及OJ组，差异有统计学意义（P<0.05）。

结论

增味小承气汤通过提升血清胃动素水平、降低血清生长抑素水平，增强了胃肠蠕动功能，加快OJ大鼠术后胃肠功能的恢复。

关键词: 增味小承气汤, 梗阻性黄疸, 肠道动力, 大鼠

Objective

To explore the effects of modified xiaochengqi decoction on gastrointestinal motility and serum levels of motilin and somatostatin levels in rats with obstructive jaundice (OJ).

Methods

The healthy SD male rats were randomly divided into the control group, sham group, surgical model of OJ+modified xiaochengqi decoction (OJ+M) group and surgical model of OJ group, with 10 rats in each group. Blood samples were collected via the caudal vein on the 3rd, 5th and 7th day after modeling and experimental intervention. ELISA assay was employed to determine serum motilin and somatostatin levels, and then intestinal propulsion test was conducted using activated carbon to observe intestinal peristalsis on the 8th day, thereby identifying the effect of modified xiaochengqi decoction on intestinal peristalsis of rats with OJ.

Results

On the 5th, 7th day after bile duct ligation, serum motilin level in OJ+M group was higher than that in sham group and OJ group, and the differences were statistically significant among groups (P<0.05). On the 3rd day after bile duct ligation, serum somatostatin level was higher in OJ+M group than that in sham group, but lower than that in control group and OJ group, with significant differences (P<0.05). The levels of serum somatostatin were increased in all rats on the 5th and 7th day after the operation, yet the serum somatostatin level was obviously lower in OJ+M group than those in other groups (P<0.05). The intestinal propelling index was 0.596±0.009 in control group, 0.470±0.021 in sham group, 0.520±0.013 in OJ+M group, and 0.350±0.008 in OJ group, while OJ+M group was higher than sham group and OJ group, and the difference was statistically significant (P<0.05).

Conclusion

Modified xiaochengqi decoction enhances gastrointestinal peristalsis and accelerates the recovery of gastrointestinal function in OJ rats by increasing serum motilin level and reducing serum somatostatin level.

Key words: Modified xiaochengqi decoction, Obstructive jaundice, Intestinal peristalsis, Rats

图1 实验流程图

表1 建模后第3、5、7天大鼠血清胃动素水平的变化(ng / L , ±s)

组别	n	第3天	第5天	第7天
正常组	10	159.231±6.891	221.494±21.487	188.540±30.799
假手术组	10	111.075±18.291	236.913±20.767	175.832±24.978
OJ+M组	10	144.193±12.196	270.463±13.749	210.720±20.620
OJ组	10	100.080±13.772	193.683±22.988	137.390±17.847
F值		42.60	25.46	16.32
P值		<0.05	<0.05	<0.05

表1 建模后第3、5、7天大鼠血清胃动素水平的变化(ng / L , ±s)

组别	n	第3天	第5天	第7天
正常组	10	159.231±6.891	221.494±21.487	188.540±30.799
假手术组	10	111.075±18.291	236.913±20.767	175.832±24.978
OJ+M组	10	144.193±12.196	270.463±13.749	210.720±20.620
OJ组	10	100.080±13.772	193.683±22.988	137.390±17.847
F值		42.60	25.46	16.32
P值		<0.05	<0.05	<0.05

表2 建模后第3、5、7天大鼠生长抑素水平的变化(ng/L, ±s)

组别	n	第3天	第5天	第7天
正常组	10	63.861±8.217	110.268±5.032	86.684±10.283
假手术组	10	44.449±11.455	121.160±17.054	78.594±13.994
OJ+M组	10	49.300±11.448	97.758±4.945	66.567±9.696
OJ组	10	65.616±7.792	133.553±9.596	97.809±5.554
F值		11.35	21.57	16.29
P值		<0.05	<0.05	<0.05

表2 建模后第3、5、7天大鼠生长抑素水平的变化(ng/L, ±s)

组别	n	第3天	第5天	第7天
正常组	10	63.861±8.217	110.268±5.032	86.684±10.283
假手术组	10	44.449±11.455	121.160±17.054	78.594±13.994
OJ+M组	10	49.300±11.448	97.758±4.945	66.567±9.696
OJ组	10	65.616±7.792	133.553±9.596	97.809±5.554
F值		11.35	21.57	16.29
P值		<0.05	<0.05	<0.05

图2 各组大鼠活性炭肠推进指数的比较　 ^*P<0.05，^**P=0.062

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Effect of modified xiaochengqi decoction on the gastrointestinal motility of rats with obstructive jaundice

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Effect of modified xiaochengqi decoction on the gastrointestinal motility of rats with obstructive jaundice