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中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (02) : 86 -90. doi: 10.3877/cma.j.issn.1647-0793.2013.02.002

所属专题: 文献

论著

YKL-40蛋白与门静脉高压脾脏肿大纤维化重塑进程的研究
王栋1, 杜锡林1, 董瑞1, 阴继凯1, 臧莉1, 江雪1, 鲁建国1,()   
  1. 1. 710038 西安,第四军医大学附属唐都医院普外科
  • 收稿日期:2013-03-18 出版日期:2013-04-01
  • 通信作者: 鲁建国

Expression and clinical significance of YKL-40 protein in human splenomegaly of portal hypertension

Dong WANG1, Xi-lin DU1, Rui DONG1, Ji-kai YIN1, Li ZANG1, Xue JIANG1, Jian-guo LU1,()   

  1. 1. Department of General Surgery, the Second Affiliated Hospital of the Fourth Military Medical University, Xi'an 710038, China
  • Received:2013-03-18 Published:2013-04-01
  • Corresponding author: Jian-guo LU
  • About author:
    Corresponding author: LU Jian-guo,Email:
引用本文:

王栋, 杜锡林, 董瑞, 阴继凯, 臧莉, 江雪, 鲁建国. YKL-40蛋白与门静脉高压脾脏肿大纤维化重塑进程的研究[J/OL]. 中华普通外科学文献(电子版), 2013, 07(02): 86-90.

Dong WANG, Xi-lin DU, Rui DONG, Ji-kai YIN, Li ZANG, Xue JIANG, Jian-guo LU. Expression and clinical significance of YKL-40 protein in human splenomegaly of portal hypertension[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(02): 86-90.

目的

探讨YKL-40蛋白在人门静脉高压脾脏组织中的表达及临床意义。

方法

采用免疫组织化学法检测人正常脾脏组织14份及门静脉高压脾脏组织48份中YKL-40蛋白表达情况,Masson三色染色检测脾脏纤维化程度。

结果

门静脉高压患者脾脏YKL-40蛋白表达明显高于正常脾脏,两组YKL-40表达差异具有统计学意义(P < 0.05),且其表达程度随Child-Pugh分级增高而增高(P < 0.05)。YKL-40蛋白表达与患者自由门静脉压力(FPP)呈正相关(R = 0.499,P < 0.01)。门静脉高压脾脏中纤维增生较正常脾脏增生明显(P < 0.05),YKL-40蛋白表达与纤维化程度呈正相关(R = 0.857,P < 0.01)。

结论

YKL-40在门静脉高压脾脏中表达增高,且表达水平与FPP、Child-Pugh分级相关。YKL-40表达与脾脏纤维化程度成正相关,提示YKL-40可能参与门静脉高压脾脏纤维化重塑过程。YKL-40蛋白可能成为治疗门静脉高压脾大的重要生物治疗因子。

Objective

To investigate the expression and clinical significance of YKL-40 protein in human splenomegaly tissue of portal hypertension.

Methods

Immunohistochemical method was used to detect the expression of YKL-40 in spleen of portal hypertension. Masson trichrome staining method was used to detect the fibrosis degrees of spleens associated with portal hypertension.

Results

Expression of YKL-40 in enlarged spleens of portal hypertension was significantly higher than that in normal spleen (P < 0.05), and its expression was significantly associated with Child-Pugh Classification (P < 0.05). Furthermore, the statistical correlation between overexpression of YKL-40 and Free Portal Pressure (FPP) was found(R = 0.499, P < 0.01). Masson trichrome staining showed that a considerable amount of fibrosis extending to the entire splenic parenchyma was found in portal hypertension patients, compared with normal people(P < 0.05). Additionally, expression of YKL-40 was correlated with fibrosis area of spleen (correlation coefficient was 0.857, P < 0.01).

Conclusions

Expression of YKL-40 in enlarged spleens of portal hypertension was significantly higher than that in normal spleen, and statistical correlation between YKL-40 with FPP and Child-Pugh Classification are found. The expression of YKL-40 is correlated with fibrosis area of spleen. YKL-40 may play an important role in molecular mechanism involved in fibrosis development of splenomegaly associated with portal hypertension.

图1 YKL-40在脾脏组织中的表达(SABC免疫组织化学法)
表1 48例门静脉高压肿大脾脏中YKL-40蛋白表达与一般临床参数比较
表2 门静脉高压脾脏YKL-40蛋白表达与临床病理参数关系
图2 YKL-40阳性表达与FPP相关关系散点图(1 cm H2O=0.098 kPa)
图3 脾脏病理Masson染色(×200)
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