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中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (02) : 91 -95. doi: 10.3877/cma.j.issn.1647-0793.2013.02.003

所属专题: 文献

论著

高迁移率族蛋白HMGA1在肝细胞癌及胆管细胞癌中的表达差异及临床意义
余元龙1, 周仕海2,(), 陈宏2, 胡泽民3, 邓辉洲2   
  1. 1. 528403 中山,中山大学附属中山医院器官移植科
    2. 528403 中山,中山大学附属中山医院普外三科
    3. 528403 中山,中山大学附属中山医院普外一科
  • 收稿日期:2012-07-01 出版日期:2013-04-01
  • 通信作者: 周仕海
  • 基金资助:
    中山市科技计划资助项目(20082A051)

Different expression of HMGA1 in hepatocarcinoma and intrahepatic cholangiocarcinoma and its significance

Yuan-long YU1, Shi-hai ZHOU2,(), Hong CHEN2, Ze-min HU3, Hui-zhou DENG2   

  1. 1. Department of Organ Transplantation, Zhongshan Hospital Affiliated to Sun Yat-sen University, Zhongshan 528403, China
  • Received:2012-07-01 Published:2013-04-01
  • Corresponding author: Shi-hai ZHOU
  • About author:
    Corresponding author: ZHOU Shi-hai, Email:
引用本文:

余元龙, 周仕海, 陈宏, 胡泽民, 邓辉洲. 高迁移率族蛋白HMGA1在肝细胞癌及胆管细胞癌中的表达差异及临床意义[J]. 中华普通外科学文献(电子版), 2013, 07(02): 91-95.

Yuan-long YU, Shi-hai ZHOU, Hong CHEN, Ze-min HU, Hui-zhou DENG. Different expression of HMGA1 in hepatocarcinoma and intrahepatic cholangiocarcinoma and its significance[J]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(02): 91-95.

目的

探讨高迁移率族蛋白A1(HMGA1)在肝细胞癌(HCC)、肝内胆管细胞癌(IHC)中的表达差异和临床意义。

方法

采用免疫组化SABC法对60例HCC、42例胆管细胞癌及10例正常肝脏组织标本中HMGA1的表达情况进行检测,并分析其与临床病理特点之间的关系。

结果

HMG组A1在10例正常肝脏组织中无一例表达,在HCC组中表达率为3.3%(2/60),在IHC组中表达率为66.7%(28/42)。IHC组与HCC组中HMGA1表达差异有统计学意义(P = 0.000);IHC组HMGA1与p53的表达呈正相关(r = 0.673,P<0.05);1年内死亡患者的HMGA1表达水平(80.0%)较1年内存活患者的(33.3%)高,差异有统计学意义(P < 0.05)。

结论

HMGA1可作为鉴别肝细胞癌与肝内胆管细胞癌的一个潜在指标。HMGA1在肝内胆管细胞癌的生成中可能发挥了重要作用,HMGA1在肝内胆管细胞癌的高表达提示预后不良。

Objective

To investigate the expression of HMGA1 in hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma(IHC) and its significance.

Methods

Immunohistochemistry (SABC method) was applied to detect HMGA1 expression in 60 HCC, 42 IHC, and 10 normal hepatic tissues. The relationship between HMGA1 expresssion and clinicpathological features of HCC and IHC was determined.

Results

No significant nuclear immunoreactivity was found in normal hepatic tissues, while 3.3% (2/60) of HCC, 66.7% (28/42) of IHC showed positive immunoreactivity, and there was significant difference between HCC and IHC(P = 0.000). The expression level of HMGA1 was strongly correlated with the expression level of p53 (r = 0.673, P < 0.05). The expression of HMGA1 in patients who died in one year (80.0%) was higher than that of patients lived(33.3%), and the difference was significant(P < 0.05).

Conclusions

HMGA1 protein level may serve as a potential diagnostic marker that differentiate HCC from IHC. HMGA1 may play an important role in the development of IHC. The high expression level of HMGA1 in IHC is strongly correlated with bad prognosis.

图1 正常肝脏组织中无HMGA1表达(DAB ×400)
图2 HMGA1在肝细胞癌中表达呈阴性(DAB ×400)
图3 HMGA1在肝细胞癌中表达呈阳性(DAB ×400)
图4 HMGA1在肝内胆管细胞癌中表达呈阴性(DAB ×400)
图5 HMGA1在肝内胆管细胞癌中表达呈阳性(DAB ×400)
表1 肝内胆管细胞癌中HMGA1的表达与临床病理的联系
表2 p53与HMGA1在肝内胆管细胞癌肝组织中的表达(例)
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