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中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (02) : 96 -100. doi: 10.3877/cma.j.issn.1647-0793.2013.02.004

所属专题: 文献

论著

整合素β6基因在HepG2细胞中主要转录调控区的定位分析
许锐锐1, 韩慧1, 贺文静1, 陈耿臻1,()   
  1. 1. 515000 汕头,汕头大学医学院第二附属医院微创医学中心
  • 收稿日期:2012-12-17 出版日期:2013-04-01
  • 通信作者: 陈耿臻

Site-directed analysis of key transcriptional regulatory regions of human integrin beta6 gene in HepG2 cells

Rui-rui XU1, Hui HAN1, Wen-jing HE1, Geng-zhen CHEN1,()   

  1. 1. Minimally Invasive Medical Center, the Second Affliated Hospital of Shantou University Medical College, Shantou, 515041, China
  • Received:2012-12-17 Published:2013-04-01
  • Corresponding author: Geng-zhen CHEN
  • About author:
    Corresponding author: CHEN Geng-zhen, Email:
引用本文:

许锐锐, 韩慧, 贺文静, 陈耿臻. 整合素β6基因在HepG2细胞中主要转录调控区的定位分析[J/OL]. 中华普通外科学文献(电子版), 2013, 07(02): 96-100.

Rui-rui XU, Hui HAN, Wen-jing HE, Geng-zhen CHEN. Site-directed analysis of key transcriptional regulatory regions of human integrin beta6 gene in HepG2 cells[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(02): 96-100.

目的

鉴定整合素β6基因在HepG2细胞中的主要转录调控区,为进一步研究αvβ6在肝癌发生发展过程中的转录调控机制奠定基础。

方法

利用β6基因转录起始位点附近1135 bp基因序列经PCR进行不同长度的缺失扩增,并连接到pGL2-basic载体,构建重组质粒,利用双荧光素酶报告基因分析系统检测启动子的转录活力,并利用Transcription Element Search System预测β6基因主要转录调控区潜在转录因子结合位点。

结果

获得4个含不同长度β6基因序列的重组质粒;当β6基因在距转录起始位点-458 bp截短到-187 bp时,转录活力降低约50%,在这个转录调控区存在YY1、AP-1和c-Myb等与肝癌相关的转录因子结合位点。

结论

在HepG2细胞中,β6基因启动子区的-458 bp/-187 bp区域是其主要的转录调控区,YY1、AP-1和c-Myb三个转录因子存在该区域,并可能是调控基因转录的重要转录因子,这将有望为肝癌的诊断和治疗提供新的治疗靶点。

Objective

To identify key transcriptional regulatory regions of human integrin beta6 (Itgβ6) gene in HepG2 cells, thus to make a preliminary study for its transcriptional regulatory mechanism.

Methods

The recombinant plasmids containing different lengths of DNA fragments upstream of translation initiation site of Itgβ6 gene as reporter promoters were constructed by deletion Method, and the promoter activities were detected using dual luciferase reporter assay system. The potential transcription factor binding sites at key transcriptional regulatory region of Itgβ6 gene were predicted by TESS.

Results

The recombinant plasmids containing different lengths of Itgβ6 gene were obtained. When the lengths of Itgβ6 gene were reduced from -458 bp to -187 bp, the transcriptional activity was decreased by about 50% . There were YY1、AP-1 and c-Myb transcriptional factor binding sites in the -458 bp to -187 bp regions.

Conclusion

The -458 bp/ -187 bp was the key transcriptional regulatory region of Itgβ6 gene in HepG2 cells, YY1、AP-1 and c-Myb are in this region, and may be the important factors for regulating the transcription of Itgβ6 gene.

图1 Itgβ6基因启动子区各重组质粒及其表达载体的凝胶电泳。
图2 Itgβ6基因启动子区各重组质粒的菌落鉴定
图3 Itgβ6基因启动子区各重组质粒的双酶切鉴定
图4 Itgβ6基因启动子区各重组质粒的测序鉴定
图5 Itgβ6基因启动子区各重组质粒在HepG2细胞中的相对荧光素酶活性检测
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