腺病毒介导NK4基因和单核细胞趋化蛋白1基因共表达对胰腺癌细胞的影响

doi:10.3877/cma.j.issn.1647-0793.2013.03.001

中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (03) : 165 -169. doi: 10.3877/cma.j.issn.1647-0793.2013.03.001

所属专题：文献；

论著

腺病毒介导NK4基因和单核细胞趋化蛋白1基因共表达对胰腺癌细胞的影响

周华平¹, 彭和平²^,^†(

), 刘启才³

^1. 510260　广州医学院第二附属医院普通外科（现在广州医学院附属肿瘤医院肿瘤外科）
^2. 510260　广州医学院第二附属医院普通外科
^3. 广州医学院

收稿日期:2013-02-05 出版日期:2013-06-01
通信作者: 彭和平

Effect of the adenovirus-mediated monocyte chemoattrarctant protein-one (MCP-1) gene and NK4 gene co-expression in pancreatic cancer cell

Hua-ping ZHOU¹, He-ping PENG²^,^†(), Qi-cai LIU³

^1. Department of General Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China

Received:2013-02-05 Published:2013-06-01
Corresponding author: He-ping PENG
About author:
Corresponding author: PENG He-ping, Email: penghp@163.com

全文 ( ) 下载PDF ( ) 导出引用

目的

探讨腺病毒介导NK4基因联合单核细胞趋化蛋白1（MCP-1）基因在胰腺癌中的共表达及对胰腺癌细胞的影响。

方法

利用重组腺病毒AD-MCP-1、AD-NK4分别单独和联合感染胰腺癌细胞株SW1990，荧光定量RT-PCR和酶联免疫吸附剂测定（ELISA）法检测NK4和MCP-1的表达情况并观察其对胰腺癌细胞和血管内皮细胞生长的影响。

结果

当感染复数（MOI）为60 efu/细胞时，SW1990细胞的感染效率接近100%，相应基因mRNA表达在AD-MCP-1组、AD-NK4组和联合组中感染后第5天达到最高，但是联合组中的MCP-1和NK4的表达水平要低于单一感染（P < 0.05）。相对于其他各组，联合感染后胰腺癌细胞的相对生长率没有明显的改变。AD-NK4组和联合组的上清均有抑制血管内皮细胞生长的作用，但两组比较差异无统计学意义。

结论

腺病毒介导NK4联合MCP-1基因感染胰腺癌SW1990细胞没有抑制作用，但NK4能够抑制血管内皮细胞的生长从而发挥抑癌作用。

关键词: 胰腺癌, 肝细胞生长因子拮抗剂, 单核细胞趋化蛋白1, 基因治疗, 腺病毒载体

Objective

To investigate the effect of the adenovirus-mediated NK4 and MCP-1 gene co-expression in pancreatic cancer cell in vitro.

Methods

NK4 gene and MCP-1 gene were singly and conjointly transfected into pancreatic cancer cells SW1990 by adenovirus vector. The expressions of NK4 and MCP-1 were detected using RT-PCR and ELISA. Then the influence on the growth of pancreatic cancer cells and vascular endothelial cells were observed.

Results

When MOI was 60 efu/cell, the infection efficiency of SW1990 was 100%. The expression of MCP-1 and NK4 mRNA were the highest in the 5th days in all the three groups. The expressions of MCP-1 and NK4 in conjoint group were lower than either single MCP-1 or single NK4 group. There was no significant difference in the growth curve of all the three and control groups. Both AD-NK4 and conjoint groups can inhibit the growth of vascular endothelial cells, but no significant difference was found between the two groups.

Conclusions

Adenovirus-mediated NK4 and MCP-1 genes can effectively infect SW1990, and did not show inhibiting effect in vitro. However, NK4 may play the role of tumor suppressor through inhibiting tumor angiogenesis.

Key words: Pancreatic neoplasms, Heptatocyte growth factor, Monocyte chemoattractant protein 1, Genetic therapy, Adenovirus

图1 病毒感染胰腺癌细胞SW1990后MCP-1和NK4基因表达水平的变化

图2 病毒感染对胰腺癌SW1990细胞生长的影响

图3 腺病毒感染对HGF诱导的血管内皮细胞生长的抑制

1	Date K, Matsumoto K, Shimura H, et al. HGF/NK4 is a specific antagonist for pleiotrophic actions of hepatocyte growth factor. FEBS Lett, 1997, 420(1): 1-6.
2	Matsumoto K, Nakamura T. NK4 (HGF-antagonist/angiogenesis inhibitor) in cancer biology and therapeutics. Cancer Sci, 2003, 94(4): 321-327.
3	Conti I, Rollins BJ. CCL2 (monocyte chemoattractant protein-1) and cancer. Semin Cancer Biol, 2004, 14(3): 149-154.
4	Coppola D. Molecular prognostic markers in pancreatic cancer. Cancer Control, 2000, 7(5): 421-427.
5	王琳，郭永章，李立, 等. 单纯疱疹病毒Ⅱ型胸苷激酶基因对人原发性肝细胞癌治疗作用的实验研究. 中华肝胆外科杂志, 2003, 9(5): 306-308.
6	Drozdzik M, Qian C, Xie X, et al. Combined gene therapy with suicide gene and interleukin-12 is more efficient than therapy with one gene alone in a murine model of hepatocellular carcinoma. J Hepatol, 2000, 32(2): 279-286.
7	Otte JM, Kiehne K, Schmitz F, et al. C-Met protooncogene expression and its regulation by cytokines in the regenerating pancreas and in pancreatic cancer cells. Scand J Gastroenterol, 2000, 35(1): 90-95.
8	Kushibiki T, Matsumoto K, Nakamura T, et al. Suppression of the progress of disseminated pancreatic cancer cells by NK4 plasmid DNA released from cationized gelatin microspheres. Pharm Res, 21(7): 1109-1118.
9	Murakami M, Nagai E, Mizumoto K, et al. Suppression of metastasis of human pancreatic cancer to the liver by transportal injection of recombinant adenoviral NK4 in nude mice. Int J Cancer, 2005, 117(1): 160-165.
10	Saimura M, Nagai E, Mizumoto K, et al. Tumor suppression through angiogenesis inhibition by SUIT-2 pancreatic cancer cells genetically engineered to secrete NK4. Clin Cancer Res, 2002, 8(10): 3243-3249.
11	Monti P, Leone BE, Marchesi F, et al. The CC chemokine MCP-1/CCL2 in pancreatic cancer progression: regulation of expression and potential mechanisms of antimalignant activity. Cancer Res, 2003, 63(21): 7451-7461.

[1]	葛盈盈, 岳金, 薛令法, 许尧祥, 赵浩然, 崔明雪, 肖文林. 靶向p38丝裂素活化蛋白激酶对兔唇裂术后瘢痕增生的影响[J/OL]. 中华口腔医学研究杂志(电子版), 2023, 17(01): 37-44.
[2]	尚培中, 张润萍, 张伟, 贾国洪, 李晓武, 苗建军, 刘冰. 梗阻性黄疸临床防治新技术单中心应用研究[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(01): 104-107.
[3]	刘恒, 侯宇川. 膀胱癌新型灌注药物的研究进展[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2023, 17(05): 445-451.
[4]	张柯迪, 潘立超, 张卓超, 王斐, 尹注增. 高危复发可切除胰腺癌新辅助治疗后机器人胰体尾癌根治术[J/OL]. 中华腔镜外科杂志(电子版), 2023, 16(03): 175-178.
[5]	王勤运, 许大彬, 张恩犁, 钱海, 赵之明. 完全脏器转位机器人胰十二指肠切除术一例[J/OL]. 中华腔镜外科杂志(电子版), 2023, 16(01): 52-55.
[6]	张一健, 赵龙, 杨扬, 张贝贝, 张译予, 张斌. 造血干/祖细胞体外扩增和临床应用[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(02): 122-127.
[7]	张瑜, 姜梦妮. 基于DWI信号值构建局部进展期胰腺癌放化疗生存获益预测模型[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 657-664.
[8]	孟煜凡, 李永政, 樊知遥, 展翰翔. 瘤内微生物在胰腺癌发病和演进中的作用机制及研究进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(04): 577-582.
[9]	王天福, 王刚. 自身免疫性胰腺炎诊治现状[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(04): 492-497.
[10]	黄金灿, 王迪, 崔松平, 陈晴, 吕少诚, 贺强, 郎韧. 预后营养指数对交界可切除胰腺癌患者术后预后的预测价值[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(01): 51-56.
[11]	孙秀丽, 刘振宇, 唐婷婷, 张景尚, 李猛, 毛迎燕, 万修华. 关注后发性白内障的发病机制及防控措施[J/OL]. 中华眼科医学杂志(电子版), 2023, 13(04): 193-198.
[12]	楚海强, 杨远游, 任刚. 胰腺癌放射治疗联合其他治疗方法的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(04): 392-396.
[13]	曹文玺, 陈箫, 竺来法, 周永平. 尼妥珠单抗联合白蛋白结合型紫杉醇治疗胰腺癌的有效性及安全性分析[J/OL]. 中华临床医师杂志(电子版), 2023, 17(04): 409-413.
[14]	陈升鑫, 翟亚奇, 卜保国, 陈德鑫, 吴浪, 方开萱, 刘文静, 李明阳, 令狐恩强. 自身免疫性胰腺炎临床特征分析[J/OL]. 中华胃肠内镜电子杂志, 2023, 10(01): 46-51.
[15]	吴子龙, 吴冰, 袁开盛, 胡瑞翔, 杨华, 王存川. 肥胖与胰腺疾病研究进展[J/OL]. 中华肥胖与代谢病电子杂志, 2024, 10(03): 213-219.

阅读次数

全文

摘要

选择文件类型/文献管理软件名称

选择包含的内容

Effect of the adenovirus-mediated monocyte chemoattrarctant protein-one (MCP-1) gene and NK4 gene co-expression in pancreatic cancer cell

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

Effect of the adenovirus-mediated monocyte chemoattrarctant protein-one (MCP-1) gene and NK4 gene co-expression in pancreatic cancer cell