腺病毒介导NK4基因和单核细胞趋化蛋白1基因共表达对胰腺癌细胞的影响

doi:10.3877/cma.j.issn.1647-0793.2013.03.001

中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (03) : 165 -169. doi: 10.3877/cma.j.issn.1647-0793.2013.03.001

所属专题：文献；

论著

腺病毒介导NK4基因和单核细胞趋化蛋白1基因共表达对胰腺癌细胞的影响

周华平¹, 彭和平²^,^†(

), 刘启才³

^1. 510260　广州医学院第二附属医院普通外科（现在广州医学院附属肿瘤医院肿瘤外科）
^2. 510260　广州医学院第二附属医院普通外科
^3. 广州医学院

收稿日期:2013-02-05 出版日期:2013-06-01
通信作者: 彭和平

Effect of the adenovirus-mediated monocyte chemoattrarctant protein-one (MCP-1) gene and NK4 gene co-expression in pancreatic cancer cell

Hua-ping ZHOU¹, He-ping PENG²^,^†(), Qi-cai LIU³

^1. Department of General Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China

Received:2013-02-05 Published:2013-06-01
Corresponding author: He-ping PENG
About author:
Corresponding author: PENG He-ping, Email: penghp@163.com

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目的

探讨腺病毒介导NK4基因联合单核细胞趋化蛋白1（MCP-1）基因在胰腺癌中的共表达及对胰腺癌细胞的影响。

方法

利用重组腺病毒AD-MCP-1、AD-NK4分别单独和联合感染胰腺癌细胞株SW1990，荧光定量RT-PCR和酶联免疫吸附剂测定（ELISA）法检测NK4和MCP-1的表达情况并观察其对胰腺癌细胞和血管内皮细胞生长的影响。

结果

当感染复数（MOI）为60 efu/细胞时，SW1990细胞的感染效率接近100%，相应基因mRNA表达在AD-MCP-1组、AD-NK4组和联合组中感染后第5天达到最高，但是联合组中的MCP-1和NK4的表达水平要低于单一感染（P < 0.05）。相对于其他各组，联合感染后胰腺癌细胞的相对生长率没有明显的改变。AD-NK4组和联合组的上清均有抑制血管内皮细胞生长的作用，但两组比较差异无统计学意义。

结论

腺病毒介导NK4联合MCP-1基因感染胰腺癌SW1990细胞没有抑制作用，但NK4能够抑制血管内皮细胞的生长从而发挥抑癌作用。

关键词: 胰腺癌, 肝细胞生长因子拮抗剂, 单核细胞趋化蛋白1, 基因治疗, 腺病毒载体

Objective

To investigate the effect of the adenovirus-mediated NK4 and MCP-1 gene co-expression in pancreatic cancer cell in vitro.

Methods

NK4 gene and MCP-1 gene were singly and conjointly transfected into pancreatic cancer cells SW1990 by adenovirus vector. The expressions of NK4 and MCP-1 were detected using RT-PCR and ELISA. Then the influence on the growth of pancreatic cancer cells and vascular endothelial cells were observed.

Results

When MOI was 60 efu/cell, the infection efficiency of SW1990 was 100%. The expression of MCP-1 and NK4 mRNA were the highest in the 5th days in all the three groups. The expressions of MCP-1 and NK4 in conjoint group were lower than either single MCP-1 or single NK4 group. There was no significant difference in the growth curve of all the three and control groups. Both AD-NK4 and conjoint groups can inhibit the growth of vascular endothelial cells, but no significant difference was found between the two groups.

Conclusions

Adenovirus-mediated NK4 and MCP-1 genes can effectively infect SW1990, and did not show inhibiting effect in vitro. However, NK4 may play the role of tumor suppressor through inhibiting tumor angiogenesis.

Key words: Pancreatic neoplasms, Heptatocyte growth factor, Monocyte chemoattractant protein 1, Genetic therapy, Adenovirus

图1 病毒感染胰腺癌细胞SW1990后MCP-1和NK4基因表达水平的变化

图2 病毒感染对胰腺癌SW1990细胞生长的影响

图3 腺病毒感染对HGF诱导的血管内皮细胞生长的抑制

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