奥曲肽对人结肠癌细胞生长增殖抑制作用的研究

doi:10.3877/cma.j.issn.1647-0793.2013.03.002

中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (03) : 170 -173. doi: 10.3877/cma.j.issn.1647-0793.2013.03.002

所属专题：文献；

论著

奥曲肽对人结肠癌细胞生长增殖抑制作用的研究

龙建武¹, 梁庆模²^,^†(

), 李峰¹, 刘龙飞¹, 周筱筠¹, 卢先洲¹

^1. 421002　衡阳，南华大学附属南华医院胃肠外科
^2. 南华大学附属第一医院肿瘤外科

收稿日期:2013-03-12 出版日期:2013-06-01
通信作者: 梁庆模
基金资助:
湖南省自然科学基金资助项目(07JJ6158)

Study of proliferation inhibition of octreotide on colonic cancer cells

Jian-wu LONG¹, Qing-mo LIANG²^,^†(), Feng LI¹, Long-fei LIU¹, Xiao-jun ZHOU¹, Xian-zhou LU¹

^1. Department of Gastrointestinal Surgery, the Affiliated Nanhua Hospital of University of South China, Hengyang 421002, China

Received:2013-03-12 Published:2013-06-01
Corresponding author: Qing-mo LIANG
About author:
Corresponding author: LIANG Qing-mo, Email: longjianwu0918@126.com

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目的

研究不同浓度的生长抑素类似物奥曲肽对人结肠癌SW480细胞生长增殖的抑制作用。

方法

体外培养人结肠癌SW480细胞，MTT比色法测定不同浓度奥曲肽抑制SW480细胞增殖作用，以效价强度最高者为最佳抑制浓度；平皿集落形成法测定奥曲肽抑制体外培养SW480细胞的锚定依赖性生长作用。

结果

不同浓度奥曲肽对SW480细胞增殖活性具有不同程度的抑制作用，在1×10^-10~1×10^-8 mol/L浓度范围内呈剂量依赖性，其中1×10^-8 mol/L为最佳抑制浓度；奥曲肽对SW480细胞锚定依赖性生长具有明显的抑制作用，并呈浓度依赖性。

结论

奥曲肽能抑制体外培养的人结肠癌SW480细胞生长增殖，并在一定范围内呈浓度与时间依赖性。

关键词: 结肠癌, 奥曲肽, 生长抑制

Objective

To study the effect of somatosatin analogue octreotide at different concentrations on proliferation in colonic cancer cell line SW480.

Methods

To cultivate colon cancer SW480 cell in vitro, MTT assay was used to analyze the effect of octreotide at different concentrations on the proliferation of SW480 cell. The best inhibiting concentration was screened which had the maximum potency. Colony formation assay on plate was used to test inhibition of clone formation in SW480 cells by octreotide(OCT).

Results

Different concentrations of OCT inhibited the proliferation of SW480 cells, in dose and time-dependent manner during the concentrations of OCT(1×10^-10 to 1×10^-8 mol/L), and the inhibitory effect of octreotide at 1×10^-8 mol/L was the most significant. Colony formation assay on plate showed that octreotide significantly inhibited the anchorage dependent growth of SW480 cells in a concentration-dependent manner.

Conclusion

OCT causes inhibition of colonic cancer cell line SW480 growth and proliferation, and the effect was dose and time dependent to a certain degree.

Key words: Colonic cancer, Octreotide, Growth suppression

表1 奥曲肽对人结肠癌SW480细胞增殖抑制作用（±s, n=6）

药物浓度(mol/L)	A₅₇₀	IR(%)
1×10^-10 PPS	1.136±0.018	0.00
1×10^-12奥曲肽	1.275±0.017^a	5.99
1×10^-10奥曲肽	1.118±0.033^b	17.52
1×10^-8奥曲肽	0.903±0.039^b	33.37
1×10^-6奥曲肽	0.879±0.030^b	35.17
5-FU（3 mg/mL）	0.491±0.046^b	64.22

表1 奥曲肽对人结肠癌SW480细胞增殖抑制作用（±s, n=6）

药物浓度(mol/L)	A₅₇₀	IR(%)
1×10^-10 PPS	1.136±0.018	0.00
1×10^-12奥曲肽	1.275±0.017^a	5.99
1×10^-10奥曲肽	1.118±0.033^b	17.52
1×10^-8奥曲肽	0.903±0.039^b	33.37
1×10^-6奥曲肽	0.879±0.030^b	35.17
5-FU（3 mg/mL）	0.491±0.046^b	64.22

图1 奥曲肽对SW480细胞增殖抑制作用

图2 奥曲肽抑制人结肠癌SW480细胞锚定依赖性生长

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