目的

探讨Caveolin-1 基因对乳腺癌细胞系Hs578T 耐药株（Hs578T/Dox）生长和凋亡的影响。

方法

在乳腺癌细胞系Hs578T 耐药株中转染Caveolin-1 基因，构建高表达Caveolin-1 蛋白的细胞系（Hs578T/Dox-cav），通过Western Blot 方法证实转染成功。将这2 种细胞以同样的条件培养96h，应用流式细胞仪检测细胞周期和凋亡的分布。 比较转染前后细胞的细胞周期分布的不同。 应用软琼脂培养法比较转染前后细胞抛锚生长能力的变化以及对促凋亡剂（staurosporine） 的不同反应。

结果

转染Caveolin-1 后明显促进了所用细胞株由G1 期向G2 期的转变（ ＜0.01）。 自然凋亡率显著下降。 抛锚生长能力增加，缓解了对staurosporine 的反应。

结论

Caveolin-1 促进乳腺癌细胞系Hs578T 耐药株生长和增殖，抑制凋亡。

Objective

Toinvestigate the effects of caveolion-1 gene on growth and apoptosis in Hs578T's doxorubicin-resistant （Hs578T/Dox） breast cancer.

Methods

The plasmid pCI-neocav-1 was introduced into Hs578T/Dox by lipofectin，and then selected single clone cells highly expressing Caveolin-1 level.Transfectants overexpression of Caveolin-1 was checked by Western blot.Cell cycle and apoptosis were analysis by flow cytometry.Cells proliferation were analysis by the soft agar clonogenic assay.

Result

The cell line overexpressed Caveolin-1 was established successfully.Transfected cells decreased markedly in G0/G1 phase （ ＜0.01） and increased significantly in S and（M+G2） phases （ ＜0.01），compared with parent Hs578T/Dox cells.overexpression of caveolin-1 enhance colony formation

Conclusion

Caveolin-1 suppresses apoptosis and promotes growth and proliferation in Hs578T/Dox cells.Caveolin-1 also.