目的

探讨巨噬细胞移动抑制因子（MIF）在肝细胞肝癌（HCC）的表达及其与HCC临床病理特征的关系。

方法

应用组织芯片技术和免疫组织化学方法检测93例HCC组织芯片癌组织MIF表达情况并分析其意义。免疫印迹（western blot）检测4对癌组织和癌旁组织MIF蛋白表达情况。

结果

MIF分布于肿瘤细胞的胞质和胞核,MIF表达率为70.97%。组织学Edmondson分级Ⅲ～Ⅳ级肝细胞癌中MIF表达率为79.45%，明显高于Ⅰ～Ⅱ级的表达40.20%（χ²=3.943,P=0.035）。在≥3.5 cm的肿瘤中MIF表达率为78.57%，明显高于在＜3.5 cm肿瘤中的表达率47.83%（χ²=7.943,P=0.005）。22例有转移的肝癌标本19例MIF阳性（86.36%），71例无转移的肝癌标本有47例MIF阳性（66.20%），发生转移的肝癌组织MIF阳性率较高，但两者差异无统计学意义（χ²=3.207,P=0.061）。MIF表达与性别、年龄、HbsAg状态和AFP水平无关。Western blot证实癌组织MIF蛋白表达明显高于癌旁组织。

结论

MIF与肿瘤大小和分化密切相关。在HCC发展进程中MIF可能促进肿瘤的生长。MIF是否促进肿瘤转移还需增加标本数及进一步的实验研究。

Objective

To explore the potential relationship between the expression of macrophage migration inhibitor factor(MIF)in hepatocellular carcinoma(HCC)tissues and clinical pathological features.

Methods

MIF expression in paraffin-embedded tissues containing 93 HCC in tissue microarray was detected with immunohistochemistry method.Western blot was used to examine the MIF expression in 4 pairs of tumor and peri-tumor tissues respectively.

Results

MIF staining was located in cytoplasm and nuclear of carcinoma cell and the positive expression rate of MIF was 70.97%in carcinoma tissue.The MIF immunostaining rate in III～Ⅳgrade of HCC(Edmondson) was 79.45%, significantly higher than thatin I～II grade of HCC(40.20%)(χ²=3.943,P=0.035).The difference of MIF expression rates(78.57%versus 47.83%)between the larger tumors(≥3.5 cm)and the smaller tumors(＜3.5 cm)was of statistically significance(P=0.005).The MIF immunostaining rates between HCC tissues with and without metastasis(86.36%versus 66.20%)was no difference(χ²=3.207,P=0.061).There was no significant difference of MIF immunostaining among different ages, genders, HBsAg situation and AFP levels.MIF expression in HCC tumor tissue (T)were significantly higher than that in peri-tumor tissue(P).

Conclusions

MIF may be potentially related to the tumor growth and differentiation.The enlargement of samples will be needed for further studies on the relationships between MIF expression and the HCC metastasis.