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中华普通外科学文献(电子版) ›› 2009, Vol. 03 ›› Issue (04) : 274 -279. doi: 10.3877/cma.j.issn.1674-0793.2009.04.004

论著

乳腺癌组织中survivin及其剪接体与VEGF的表达及临床意义
章乐虹1,(), 杨基鹏1, 彭艳华1, 贺勇1   
  1. 1.510260 广州医学院第二附属医院乳腺外科
  • 收稿日期:2009-02-19 出版日期:2009-08-01
  • 通信作者: 章乐虹
  • 基金资助:
    广东省科技计划项目(2005B31201012)

Expression of survivin and its splicing variants and VEGF in human breast cancer tissue and their clinical significance

Le-hong ZHANG1,(), Ji-peng YANG1, Yan-hua PENG1, Yong HE1   

  1. 1.Department of Genery Surgery, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China
  • Received:2009-02-19 Published:2009-08-01
  • Corresponding author: Le-hong ZHANG
引用本文:

章乐虹, 杨基鹏, 彭艳华, 贺勇. 乳腺癌组织中survivin及其剪接体与VEGF的表达及临床意义[J]. 中华普通外科学文献(电子版), 2009, 03(04): 274-279.

Le-hong ZHANG, Ji-peng YANG, Yan-hua PENG, Yong HE. Expression of survivin and its splicing variants and VEGF in human breast cancer tissue and their clinical significance[J]. Chinese Archives of General Surgery(Electronic Edition), 2009, 03(04): 274-279.

目的

研讨抗凋亡蛋白survivin及其剪接体survivin-△Ex3、survivin-2B与血管内皮生长因子(VEGF)在乳腺癌组织中表达情况及其与临床病理学指标的关系。

方法

以RT-PCR法检测100例乳腺癌及癌旁5 cm正常乳腺组织标本中survivin及其剪接体survivin-△Ex3、survivin-2B与VEGF基因的mRNA表达,半定量分析电泳结果。以免疫组化法检测乳腺癌石蜡标本中的雌激素受体(ER)、孕激素受体(PR)和HER-2基因状态。

结果

Survivin及其剪接体survivin-△Ex3、survivin-2B mRNA在乳腺癌组织中均有较高表达,在癌旁5 cm正常乳腺组织中均低表达,差异有统计学意义(P<0.05);VEGF mRNA在乳腺癌组织中均有较高表达,在正常乳腺组织中均低表达,差异有统计学意义(P<0.05);淋巴结转移与survivin-△Ex3、survivin-2B的表达相关(P<0.05)。

结论

Survivin及其剪接体survivin-△Ex3、survivin-2B与VEGF的表达与乳腺癌发生、发展有关,与其他预后指标ER、PR、HER-2等的联合检测可望对患者的预后作出更准确的预测。

Objective

To investigate the expression of survivin and its splicing variants survivin-△Ex3,survivin-2B and VEGF mRNA in human breast cancer and its relation to clinicopathological and parameters.

Methods

The expression of survivin and its splicing variants survivin-△Ex3, survivin-2B and VEGF mRNA was determined by both reverse transcription-polymerase chain reaction (RT-PCR)and semi-quantitative electrophoresis in 100 breast cancer and adjacent normal breast tissue.The protein expression of estrogen receptor(ER),progesterone receptor (PR),Her-2 gene was detected by immuno-histochemical method.

Results

The expressions of survivin and its splicing variants survivin-△Ex3, survivin-2B mRNA in the breast cancer tissues were significantly higher than the adjacent normal breast tissues(P<0.05).The expressions of VEGF mRNA in the breast cancer tissues was significantly higher than the adjacent normal breast tissues (P<0.05).The expression of survivin-△Ex3, survivin-2B mRNA positively correlated with lymph node metastasis (P<0.05).

Conclusions

The expression of survivin and its splicing variants survivin-△Ex3, survivin-2B and VEGF are correlated with breastcancercarcinogenesisanddevelopment.Itmaybeusedtogetherwithotherprognosticmarkers(ER,PR,HER-2)in the prognosis and of breastcancer.

表1 乳腺癌组织中survivin 及其剪接体与VEGF 的上下游引物
图1 Survivin 及其剪接体survivin-△Ex3、survivin-2B mRNA 在癌组织中的电泳图
图2 Survivin 及其剪接体survivin-△Ex3、survivin-2B mRNA 在癌旁正常组织中的电泳图
图3 VEGF mRNA 在癌组织中的电泳图
图4 VEGF mRNA 在癌旁正常组织中的电泳图
表2 Survivin 及其剪接体与VEGF mRNA 的阳性表达(例)
表3 Survivin 及其剪接体与VEGF mRNA 的表达与临床病理学指标的关系(例)
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