目的

CD44是细胞表面黏附分子，与乳腺癌的转移和预后关系密切，但其与乳腺癌化疗耐药的关系尚未明确。本研究主要探讨CD44与乳腺癌化疗耐药的关系。

方法

采用SP免疫组化的方法检测73例乳腺癌患者肿瘤组织CD44的表达，分析新辅助化疗敏感患者与耐药患者化疗前后CD44的表达情况；用RT-PCR和流式细胞免疫的方法检测乳腺癌细胞株MCF-7与其对应的阿霉素耐药细胞株MCF-7/ADR在mRNA和蛋白水平CD44的表达，并分析其差异。

结果

乳腺癌新辅助化疗耐药患者化疗前肿瘤组织CD44表达强于化疗敏感患者（χ²=4.22，P<0.05）；耐药患者化疗后肿瘤组织CD44表达亦强于敏感患者(χ²=9.954，P<0.01）；化疗前后比较发现化疗后肿瘤组织CD44表达较化疗前增高（t＝3.43，P<0.01）；耐药细胞株mRNA和蛋白水平CD44表达都升高。

结论

CD44表达与乳腺癌化疗耐药密切相关，可作为乳腺癌化疗耐药的预测指标，化疗可富集CD44阳性表达的肿瘤细胞。

Objective

CD44 is an adhesion molecule of cell surface, which is closely related with metastasis and prognosis of breast cancer,but the relationship between CD44 and drug resistance of breast cancer chemotherapy is not clear so far. We mainly study the relationship between CD44 and drug resistance of breast cancer chemotherapy.

Methods

The expression of CD44 was detected by SP immunohistochemical methods,the different expression of CD44 was investigated between drug-sensitive groups and drug-resistant groups of neoadjuvant chemotherapy,before and after chemotherapy.The different expressions of CD44 on the level of mRNA and protein in MCF-7 cell line and drug-resistant cell lines MCF-7/ADR was tested by reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry.

Results

Expression of CD44 before chemotherapy in drug-resistant groups was much higher than drug-sensitive ones(χ²=4.22，P <0.05). This was also found after chemotherapy(χ²=9.954，P<0.01).The expression of CD44 after chemotherapy was higher than that before chem otherapy (t＝3.43，P<0.01).Expression of mRNA and protein of CD44 in MCF-7/ADR cell lines was higher than drug-sensitive cell lines (MCF-7).

Conclusion

CD44 may be conferred as a predictive index of breast cancer chemotherapy drug resistance,and chemotherapy may enrich CD44 positive breast cancer cell.