观察紫杉醇在大鼠原位肝移植中的作用及其对细胞毒性T淋巴细胞（CTL）的影响。

建立Wistar→SD大鼠肝移植的急性排斥模型。将大鼠分为对照组（阻断全肝血流）、急排组（Wistar→SD肝移植）、紫杉醇组（Wistar→SD肝移植，紫杉醇腹腔注射0.75 mg·kg^-1·d^-1，观察生存时间、病理改变、生化指标及CTL、T淋巴细胞凋亡情况。

紫杉醇组大鼠的术后存活时间高于急排组，[（18.2±1.5）d vs（8.8±1.9）d，P<0.01）。急排组的HE评分（Banff）高于紫杉醇组（7.80±0.83 vs 4.80±0.82，P<0.01）。紫杉醇组的白蛋白水平高于急排组（P=0.003）；紫杉醇组的谷丙转氨酶水平低于急排组（t=25.23，P<0.01）。CTL检测中，急排组的特异性杀伤靶细胞的百分率均高于紫杉醇组（P<0.01）。急排组的T淋巴细胞的凋亡率低于紫杉醇组[（7.16±1.56）% vs（16.47±2.51）%，P<0.01]。

紫杉醇在大鼠肝移植中具有免疫抑制作用，可能通过诱导T淋巴细胞凋亡来抑制T淋巴细胞的活化发挥作用。

To observation of the effect of paclitaxol in rat orthotopic liver transplantation and its impact on the cytotoxic T lymphocyte (CTL).

Wistar → SD rat liver transplantation model of acute rejection was established. The rats were divided into three groups: control group (blocking total hepatic blood flow), acute rejection group (Wistar → SD liver transplantation), paclitaxel group (Wistar → SD liver transplantation, paclitaxel intraperitoneal injection of 0.75 mg·kg^-1·d^-1. The survival time, pathological changes, biochemical markers and CTL, T lymphocyte apoptosis were observed.

The postoperative survival time of paclitaxel group were rats higher than acute rejection group, (18.2±1.5)d vs(8.8± 1.9) d, P<0.01. HE score (Banff) of acute rejection group is higher than the paclitaxel group, 7.80±0.83 vs 4.80±0.82, t=5.67, P<0.01. Albumin level in paclitaxel group ranked higher than acute rejection group, P=0.003, while alanine aminotransferase of paclitaxel group were lower than acute rejection group, P<0.01. In CTL, the percentage of anti-target cell in acute rejection group were higher than the percentage of paclitaxel group, P<0.01. Tlymphocyte apoptosis rate of acute rejection group isless than the paclitaxel group, (7.16±1.56)% vs(16.47±2.51)%, P<0.01.

Paclitaxel exerts immunosuppression in rat liver transplantation, by inducing T lymphocyte apoptosis to suppress T lymphocyte activation probably.