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中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (04) : 284 -288. doi: 10.3877/cma.j.issn.1674-0793.2013.04.010

所属专题: 文献

论著

严重腹腔感染时血、粪纳米细菌的检测及其与SIRS关系的实验研究
黄美泰1, 伍颖君2, 郑永波3, 钟俊新4, 段雪飞4, 李晶晶4, 吴承堂4,()   
  1. 1. 528226 佛山,南海区罗村医院外科
    2. 江门中心医院普通外科
    3. 中国人民解放军第260医院普通外科
    4. 南方医科大学南方医院惠侨楼普通外科
  • 收稿日期:2013-07-05 出版日期:2013-08-01
  • 通信作者: 吴承堂

Experimental studies on the relationship between nanobacteria translocation and SIRS of rats which suffering critical infection of abdominal cavity

Mei-tai HUANG1, Ying-jun WU2, Yong-bo ZHENG3, Jun-xin ZHONG4, Xue-fei DUAN4, Jing-jing LI4, Cheng-tang WU4,()   

  1. 1. Department of General Surgery, Nanhai District Luocun Hospital of Foshan, Foshan 528226, China
  • Received:2013-07-05 Published:2013-08-01
  • Corresponding author: Cheng-tang WU
  • About author:
    Corresponding author:WU Cheng-tang, Email:
引用本文:

黄美泰, 伍颖君, 郑永波, 钟俊新, 段雪飞, 李晶晶, 吴承堂. 严重腹腔感染时血、粪纳米细菌的检测及其与SIRS关系的实验研究[J/OL]. 中华普通外科学文献(电子版), 2013, 07(04): 284-288.

Mei-tai HUANG, Ying-jun WU, Yong-bo ZHENG, Jun-xin ZHONG, Xue-fei DUAN, Jing-jing LI, Cheng-tang WU. Experimental studies on the relationship between nanobacteria translocation and SIRS of rats which suffering critical infection of abdominal cavity[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(04): 284-288.

目的

探讨严重腹腔感染时血、粪中纳米细菌(nanobacteria, NB)的检测情况及其与全身炎症反应综合征(SIRS)的关系。

方法

40只SD大鼠采用盲肠结扎加穿孔手术(CLP)制作严重腹腔感染模型,分别于术前、术后2 d取血、粪标本进行NB培养,电镜下观察NB并加以鉴定。另取回肠组织作病理观察。

结果

造模后的大鼠符合SIRS标准,病理观察肠黏膜层部分区域有黏膜上皮脱落,肠上皮细胞间紧密连接出现异常,结构松弛。与CLP手术前比较,术后2 d体温(℃)显著升高(39.98±0.43 vs 38.37±0.34,t = 18.538,P < 0.01),血液白细胞计数(×109/L)亦明显增高(21.13±3.27 vs 10.43±2.23,t = 15.909,P < 0.01)。严重腹腔感染前、后血电镜下NB阳性率分别为17.5%和42.5%(χ2 = 5.952,P = 0.015),粪NB阳性率分别为90.0%和92.5%,差异无统计学意义(χ2 = 0.156,P = 0.692)。

结论

严重腹腔感染SD大鼠血中纳米细菌较感染前显著增加,可能与SIRS的发生发展有关。

Objective

To explore the relationship between nanobacteria(NB) translocation from blood and stool and SIRS of rats suffering critical infection of abdominal cavity.

Methods

Forty SD rats were involved in the study. The models of critical abdominal cavity infection were made by cecal ligation plus puncture (CLP). The test samples of blood and stool were obtained before and two days after infected, respectively. The samples were cultured with NB culture medium and identified by transmission electron microscopy (TEM). The morphological change of the pathologic lesion in the ileum tissue of the rats were observed.

Results

Two days after infected, it showed epithelia abscission and tight junction loosening in some area of intestinal mucosa of the rats. Compared with preoperative, the average rectal temperature (℃) of the experimental group rats was higher significantly (39.98±0.43 vs 38.37±0.34, t = 18.538, P < 0.01), and the average numer of leukocyte (×109/L) was higher significantly(21.13±3.27 vs 10.43±2.23, t = 15.909, P < 0.01) at postoperative 2 d. The positive rates of NB were 17.5% and 42.5% (χ2 = 5.952, P = 0.015) in the blood samples before and after CLP 2 d respectively by TEM, and that were 90.0% and 92.5% (χ2 = 0.156, P = 0.692) in the stool.

Conclusion

The positive rates of NB increases in the blood samples, which may be associated with SIRS induced by critical abdominal cavity infection.

图1 光学显微镜下SD大鼠回肠黏膜
图2 光学显微镜下SD大鼠回肠黏膜
图3 SD大鼠腹腔感染后粪标本中电镜下
图4 SD大鼠腹腔感染后血标本中电镜下
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