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中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (05) : 350 -353. doi: 10.3877/cma.j.issn.1674-0793.2013.05.005

所属专题: 文献

论著

P-糖蛋白表达与原发性肝癌合并门静脉癌栓患者术后辅助化疗效果的相关性研究
胡文杰1, 黎东明1, 陈伟1, 张昆松1, 黄力1, 梁力建1,()   
  1. 1. 510080 广州,中山大学附属第一医院肝胆外科
  • 收稿日期:2013-04-04 出版日期:2013-10-01
  • 通信作者: 梁力建

Correlation between the expression of P-gp and efficacy of chemotherapy postoperation in hepatocellular carcinoma patients with tumor thrombi in major portal vein

Wen-jie HU1, Dong-Ming LI1, Wei CHEN1, Kun-song ZHANG1, Li HUANG1, Li-jian LIANG1,()   

  1. 1. Department of Hepatobilliary Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
  • Received:2013-04-04 Published:2013-10-01
  • Corresponding author: Li-jian LIANG
  • About author:
    Corresponding author: LIANG Li-jian, Email:
引用本文:

胡文杰, 黎东明, 陈伟, 张昆松, 黄力, 梁力建. P-糖蛋白表达与原发性肝癌合并门静脉癌栓患者术后辅助化疗效果的相关性研究[J/OL]. 中华普通外科学文献(电子版), 2013, 07(05): 350-353.

Wen-jie HU, Dong-Ming LI, Wei CHEN, Kun-song ZHANG, Li HUANG, Li-jian LIANG. Correlation between the expression of P-gp and efficacy of chemotherapy postoperation in hepatocellular carcinoma patients with tumor thrombi in major portal vein[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(05): 350-353.

目的

研究P-糖蛋白(P-gp)表达与原发性肝细胞癌合并门静脉癌栓患者表达情况与临床化疗效果之间的关系。

方法

收集术后接受化疗的29例原发性肝细胞癌合并门静脉癌栓患者的石蜡块,构建组织芯片。采用免疫组化染色技术,检测P-gp蛋白的表达情况。根据P-gp蛋白表达强弱分为耐药组和非耐药组,进行生存分析。

结果

P-gp蛋白表达的阳性率为75.86%(22/29)。中位无瘤生存期在耐药组(20例)与非耐药组(9例)间有显著差别。多因素Cox回归分析显示P-gp表达情况为无瘤生存期的独立影响因子。

结论

P-gp的强表达是导致肝癌耐药、化疗效果欠佳的原因之一,P-gp有可能作为临床筛选化疗患者的指标。

Objective

To investigate the expression of P-glycoprotein (P-gp) and its correlation to chemotherapeutic efficacy in hepatocellular carcinoma (HCC) with tumor thrombi in major portal vein.

Methods

Twenty-nine patients of HCC with tumor thrombi in major portal vein following hepatectomy and thrombectomy were enrolled in this study, all the patients received immunochemotherapy postperatively. Specimens embedded in paraffin were collected for the construction of tissue microarrays. P-gp protein were visualized by immunohistochemical staining using Monoclonal antibodies JSB-1. The patients were divided into chemotherapy sensitive and resistant groups according to the staining grade. The intensity≥(++) were considered resistant to chemotherapy. Survival time was compared between the two groups and prognostic factors were identified using Cox proportional hazards model.

Results

The positive rate of P-gp was 75.86% (22/29). Medium tumor-free survival time showed significant differences between two groups. Multivariate analysis revealed that the expression grade of P-gp was independent prognostic factor for tumor-free survival time.

Conclusion

In HCC with tumor thrombi in major portal vein, the overexpression of P-gp is one of the major reason for resistance to chemotherapy, detection of which will be helpful in chemotherapy decision making.

图1 P-gp蛋白阳性表达,细胞膜上可见棕黄色染色颗粒(免疫组织化学染色 ×400)
图2 不同P-gp蛋白表达的肝癌患者无瘤生存曲线20例P-gp(++)者为耐药组,其余9例为非耐药组,P=0.021
1
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