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中华普通外科学文献(电子版)

中华普通外科学文献(电子版) ›› 2013, Vol. 07 ›› Issue (05) : 347 -349. doi: 10.3877/cma.j.issn.1674-0793.2013.05.004

所属专题： 文献；

论著

2-脱氧-D-葡萄糖联合氟尿嘧啶对胃癌细胞MGC-803凋亡影响的实验研究
章斐然1, 李威1,†(), 郑仰1, 陈君填1   
  1. 1. 515041　汕头大学医学院第一附属医院
  • 收稿日期:2013-06-23 出版日期:2013-10-01
  • 通信作者: 李威
  • 基金资助:
    广东省科技计划项目(2011B031800189)

Effects of 2-deoxy-D-glucose and 5-Fu on proliferation and apoptosis of human gastric cancer MGC-803 cells

Fei-ran ZHANG1, Wei LI1,†(), Yang ZHENG1, Jun-tian CHEN1   

  1. 1. Department of General Surgery, First Affiliated Hospital, ShanTou University Medical College, Shantou 515041, China
  • Received:2013-06-23 Published:2013-10-01
  • Corresponding author: Wei LI
  • About author:
    Corresponding author: LI Wei, Email:
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引用本文：

章斐然, 李威, 郑仰, 陈君填. 2-脱氧-D-葡萄糖联合氟尿嘧啶对胃癌细胞MGC-803凋亡影响的实验研究[J/OL]. 中华普通外科学文献(电子版), 2013, 07(05): 347-349.

Fei-ran ZHANG, Wei LI, Yang ZHENG, Jun-tian CHEN. Effects of 2-deoxy-D-glucose and 5-Fu on proliferation and apoptosis of human gastric cancer MGC-803 cells[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(05): 347-349.

目的

探讨糖酵解抑制剂2-脱氧-D-葡萄糖（2-DG）与氟尿嘧啶（5-Fu）单独及联合应用对人胃癌细胞MGC-803增殖及凋亡的影响。

方法

实验设立2-DG组、5-Fu组、联合组（2-DG +5-Fu）及空白对照组，将2-DG（10 mmol/L）及5-Fu（5.0 μg/L）单药及联合用药作用于人胃癌细胞MGC-803，MTT法检测各组MGC-803细胞的增殖抑制率，流式细胞仪检测各组细胞凋亡情况。

结果

2-DG和5-Fu均可使胃癌细胞增殖受到抑制并可诱导细胞凋亡，两药合用后其作用明显增强。

结论

2-DG能有效抑制人胃癌细胞MGC-803细胞生长增殖，并诱导其凋亡；2-DG与5-Fu联合应用抗肿瘤作用明显增强。

关键词: 2-脱氧-D-葡萄糖, 胃癌, 细胞凋亡
Objective

To investigate the effects of 2-deoxy-D-glucose ( 2-DG) or 5-Fu alone and the combination of both on the proliferation and apoptosis of human gastric cancer MGC-803 cells.

Methods

Human gastric cancer MGC-803 cells were treated with 2-DG or 5-Fu alone, or both. The inhibitory effect on the proliferation of MGC-803 cells was detected by MTT method. Apoptotic rate was assayed by flow cytometry.

Results

2-DG or 5-Fu inhibited the growth of MGC-803 cells obviously and induced the apoptosis. When 2-DG and 5-Fu were combined, the inhibitory effect and apoptotic rate were improved (P<0.05).

Conclusions

2-DG inhibits the growth of human gastric cancer MGC-803 cells. The combination of 2-DG and 5-Fu improves the ability of 5-Fu to attack the tumor cells.

Key words: 2-deoxy-D-glucose, Gastric cancer, Apoptosis
1
耿翔，周奇，刘超, 等. 2-脱氧-D-葡萄糖与奥沙利铂对人肝癌SMMC-7721细胞增殖及凋亡的影响. 中国病理生理杂志, 2011, 27(11): 2101-2105.
2
韩保卫，刘光辉，董帅军, 等. 糖酵解抑制剂对低氧胰腺癌细胞增殖和凋亡的影响. 中国普通外科杂志, 2009, 18(3): 243-246.
3
程秀，刘浩，方琳, 等. 2-DG增强乳腺癌细胞对阿霉素化疗敏感性的作用. 中国药理学通报, 2010, 26(10): 1371-1376.
4
Sottnik JL, Lori JC, Rose BJ, et al. Glycolysis inhibition by 2-deoxy-D-glucose reverts the metastatic phenotype in vitro and in vivo. Clin Exp Metastasis, 2011, 28(8): 865-875.
5
Yamaguchi R, Perkins G. Finding a Panacea among combination cancer therapies. Cancer Res, 2012, 72(1): 18-23.
6
Estañ MC, Calviño E, de Blas E, et al. 2-Deoxy-D-glucose cooperates with arsenic trioxide to induce apoptosis in leukemia cells: involvement of IGF-1R-regulated Akt/mTOR, MEK/ERK and LKB-1/AMPK signaling pathways. Biochem Pharmacol, 2012, 84(12): 1604-1616.
7
Gupta S, Farooque A, Adhikari JS, et al. Enhancement of radiation and chemotherapeutic drug responses by 2-deoxy-D -glucose in animal tumors. J Cancer Res Ther, 2009, 5 Suppl 1: S16-20.
8
Hadzic T, Aykin-Burns N, Zhu Y, et al. Paclitaxel combined with inhibitors of glucose and hydroperoxide metabolism enhances breast cancer cell killing via H2O2-mediated oxidative stress. Free Radic Biol Med, 2010, 48(8): 1024-1033.
9
Bhardwaj R, Sharma PK, Jadon SP, et al. A combination of 2-deoxy-D-glucose and 6-aminonicotinamide induces cell cycle arrest and apoptosis selectively in irradiated human malignant cells. Tumour Biol, 2012, 33(4): 1021-1030.
10
Aghaee F, Pirayesh Islamian J, Baradaran B. Enhanced radiosensitivity and chemosensitivity of breast cancer cells by 2-deoxy-d-glucose in combination therapy. J Breast Cancer, 2012, 15(2): 141-147.
11
Heyfets A, Flescher E. Cooperative cytotoxicity of methyl jasmonate with anti-cancer drugs and 2-deoxy-D-glucose. Cancer Lett, 2007, 250(2): 300-310.
12
Merchan JR, Kovács K, Railsback JW, et al. Antiangiogenic activity of 2-deoxy-D-glucose. PLoS One, 2010, 5(10): e13699.
13
Cheng G, Zielonka J, Dranka BP, et al. Mitochondria-targeted drugs synergize with 2-deoxyglucose to trigger breast cancer cell death. Cancer Res, 2012, 72(10): 2634-2644.
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