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中华普通外科学文献(电子版) ›› 2021, Vol. 15 ›› Issue (04) : 258 -262. doi: 10.3877/cma.j.issn.1674-0793.2021.04.004

论著

SDF-1α基因多态性与西北地区汉族人群胃癌发病风险的相关性研究
肖琪1, 普彦淞1, 都庆国1, 段降龙1, 李晓帆1, 杨妮1, 薛飞1,()   
  1. 1. 710068 西安,陕西省人民医院普外二科
  • 收稿日期:2021-03-14 出版日期:2021-08-03
  • 通信作者: 薛飞
  • 基金资助:
    陕西省自然科学基础研究计划项目(2019JQ-973,2020JQ-947)

Association between SDF-1α gene polymorphism and risk of gastric cancer in the Han nationality of Northwest China

Qi Xiao1, Yansong Pu1, Qingguo Du1, Xianglong Duan1, Xiaofan Li1, Ni Yang1, Fei Xue1,()   

  1. 1. The Second Department of General Surgery, Shaanxi Provincial People’s Hospital, Xi’an 710068, China
  • Received:2021-03-14 Published:2021-08-03
  • Corresponding author: Fei Xue
引用本文:

肖琪, 普彦淞, 都庆国, 段降龙, 李晓帆, 杨妮, 薛飞. SDF-1α基因多态性与西北地区汉族人群胃癌发病风险的相关性研究[J/OL]. 中华普通外科学文献(电子版), 2021, 15(04): 258-262.

Qi Xiao, Yansong Pu, Qingguo Du, Xianglong Duan, Xiaofan Li, Ni Yang, Fei Xue. Association between SDF-1α gene polymorphism and risk of gastric cancer in the Han nationality of Northwest China[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(04): 258-262.

目的

探讨SDF-1α基因3’非翻译区801位点A等位基因(rs1801157,SDF-1α G801A)多态性与西北地区汉族人群胃癌的发病风险是否存在显著相关性。

方法

收集2016年6月至2018年7月陕西省人民医院收治的胃癌患者131例和健康对照者76名,均为汉族。分析研究对象的临床病理资料,通过限制性片段长度多态性检测技术,利用MspⅠ限制酶酶切PCR扩增SDF-1基因801位点附近DNA片段,分析检测外周血SDF-1α G801A位点的单核苷酸多态性(SNPs)。

结果

SDF-1α G801A与胃癌的发病有明显相关性,G/G基因型(P=0.002,OR=0.40,95% CI:0.22~0.71)与G/A(P=0.002,OR=2.52,95% CI:1.41~4.52)与胃癌的易感性显著相关。进一步分层分析发现,SDF-1α G/A突变人群在感染幽门螺杆菌情况下其胃癌发病风险显著增加(P<0.05)。

结论

SDF-1α G801A基因多态性与西北地区汉族人群胃癌发病风险明显相关,特别是感染幽门螺杆菌的G/A基因型人群胃癌发病风险显著增加。

Objective

To explore whether there is significant correlation between the SDF-1α G801A polymorphism and the risk of gastric cancer in the Han nationality of Northwest China.

Methods

A total of 131 cases with gastric cancer and 76 healthy controls were enrolled from Shaanxi Provincial People’sHospital between June 2016 and July 2018. The clinical and pathological data of the subjects were analyzed. The DNA fragment near 801 site of SDF-1 gene was amplified by restriction enzyme digestion PCR with MSP, and single nucleotide polymorphisms (SNPs) of SDF-1α G801A locus in the peripheral blood were detected.

Results

The SDF-1α G801A polymorphism was significantly related to the risk of gastric cancer. The genotype G/G (P=0.002, OR=0.40, 95% CI: 0.22-0.71) and G/A (P=0.002, OR=2.52, 95% CI: 1.41-4.52) were significantly related to the susceptibility of gastric cancer. Further stratified analysis found that the SDF-1α G/A mutant population had a significantly increased risk of gastric cancer when infected with H.pylori (P<0.05).

Conclusion

G801A polymorphism of SDF-1α gene is significantly associated with the risk of gastric cancer in the Han nationality of Northwest China, and the risk of gastric cancer with H.pylori infection is significantly higher in people with the G/A genotype.

表1 研究对象的临床病理特征比较[例/名(%)]
表2 SDF-1α G801A基因多态性与胃癌发病风险的相关性[例/名(%) ]
表3 胃癌组和对照组SDF-1α G801A基因多态性与Hp感染相关性分析[例/名(%) ]
表4 胃癌发病独立危险因素分析
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