切换至 "中华医学电子期刊资源库"

中华普通外科学文献(电子版) ›› 2021, Vol. 15 ›› Issue (04) : 269 -272. doi: 10.3877/cma.j.issn.1674-0793.2021.04.006

论著

BANCR调控VEGF-C/VEGFR-3通路促进胰腺癌微淋巴管生成
郝少龙1, 韩威,1, 纪宇1, 孙浩2, 石浩伟1, 马纪红3   
  1. 1. 101149 北京,首都医科大学附属北京潞河医院普外科
    2. 101149 北京,首都医科大学附属北京潞河医院中心实验室
    3. 101149 北京,首都医科大学附属北京潞河医院医疗保健病区
  • 收稿日期:2020-12-10 出版日期:2021-08-03
  • 通信作者: 韩威
  • 基金资助:
    北京市通州区科委科技创新专项(KJ2019CX014-05); 北京市通州区科技计划项目(KJ2020 CX006-10)

BANCR promoting lymphangiogenesis by regulating VEGF-C/VEGFR-3 pathway in pancreatic cancer

Shaolong Hao1, Wei Han,1, Yu Ji1, Hao Sun2, Haowei Shi1, Jihong Ma3   

  1. 1. Department of General Surgery, the Affiliated Beijing Luhe Capital University of Medical Sciences, Beijing 101149, China
    2. Department of Central Laboratory, the Affiliated Beijing Luhe Capital University of Medical Sciences, Beijing 101149, China
    3. Department of Health Care Ward, the Affiliated Beijing Luhe Capital University of Medical Sciences, Beijing 101149, China
  • Received:2020-12-10 Published:2021-08-03
  • Corresponding author: Wei Han
引用本文:

郝少龙, 韩威, 纪宇, 孙浩, 石浩伟, 马纪红. BANCR调控VEGF-C/VEGFR-3通路促进胰腺癌微淋巴管生成[J/OL]. 中华普通外科学文献(电子版), 2021, 15(04): 269-272.

Shaolong Hao, Wei Han, Yu Ji, Hao Sun, Haowei Shi, Jihong Ma. BANCR promoting lymphangiogenesis by regulating VEGF-C/VEGFR-3 pathway in pancreatic cancer[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(04): 269-272.

目的

研究BANCR在胰腺癌中的表达及其对淋巴管生成作用机制。

方法

实时荧光定量PCR(qPCR)检测胰腺癌细胞(SW1990、PANC-1)及人胰腺导管上皮细胞(HPDC)中BANCR的表达;应用siRNA干扰胰腺癌细胞BANCR的表达,将转染后的胰腺癌细胞与人真皮淋巴管内皮细胞(HDLEC)进行3D共培养,并统计微淋巴管密度(MLVD)。应用qPCR检测转染后的BANCR-siRNA组和空载质粒NC组胰腺癌细胞中VEGF-C、VEGFR-3 mRNA的相对表达量。

结果

与HPDC的1.02±0.222相比,胰腺癌细胞PANC-1和SW1990中BANCR显著高表达,分别为10.03±3.356、10.37±1.459(P<0.001)。干扰BANCR表达后BANCR-siRNA组的胰腺癌细胞MLVD较NC组显著降低(SW1990:3.87±1.767 vs 18.00±6.130,P<0.001;PANC-1:5.27±2.631 vs 16.80±3.764,P<0.001)。且BANCR-siRNA组中VEGF-C及VEGFR-3转录水平较NC组显著下调(VEGF-C:1.35±0.926 vs 6.97±3.677,P=0.011;VEGFR-3:0.98±0.635 vs 2.88±1.422,P=0.026)。

结论

BANCR在胰腺癌细胞中表达上调,并可能通过调控VEGF-C/VEGFR-3通路促进胰腺癌淋巴管生成和淋巴结转移,有望为胰腺癌的诊治提供新靶点。

Objective

To investigate the expression of BANCR in pancreatic carcinoma and its mechanism on lymphangiogenesis.

Methods

The expression of BANCR in pancreatic cancer cells (SW1990, PANC-1) and human pancreatic ductal epithelial cell (HPDC) was detected by qPCR. siRNA was used to interfere with the expression of BANCR in pancreatic cancer cells. The transfected pancreatic cancer cells and human dermal lymphatic endothelial cells (HDLEC) were co-cultured in 3D, and micro-lymphatic vessel density (MLVD) was calculated. qPCR was used to detect the relative expression of VEGF-C and VEGFR-3 mRNA in pancreatic cancer cells of BANCR-siRNA group and NC group.

Results

Compared with 1.02±0.222 of HPDC, the expression of BANCR in PANC-1 and SW1990 cells was significantly higher (10.03±3.356, 10.37±1.459, respectively) (P<0.001). The pancreatic cancer cell MLVD in the BANCR-siRNA group was significantly lower than that in the NC group (SW1990: 3.87±1.767 vs 18.00±6.130, P<0.001; PANC-1: 5.27±2.631 vs 16.80±3.764, P<0.001). And the transcriptional level of VEGF-C and VEGFR-3 in the BANCR-siRNA group was significantly lower than that in the NC group (VEGF-C: 1.35±0.926 vs 6.97±3.677, P=0.011; VEGFR-3: 0.98±0.635 vs 2.88±1.422, P=0.026).

Conclusion

BANCR is up-regulated in pancreatic carcinoma cells and may play an important role in lymphangiogenesis and lymph node metastasis in pancreatic carcinoma by regulating VEGF-C/VEGFR-3 pathway, which is expected to provide a new target for clinical diagnosis, treatment and prognosis of pancreatic carcinoma.

表1 引物序列表
图2 BANCR对胰腺癌细胞淋巴管生存的影响 A为胰腺癌细胞株PANC-1、SW1990中BANCR-siRNA与未经siRNA干扰的NC组淋巴管生成情况(×100);B为几种细胞株中微淋巴管密度(MLVD)的比较;C为PC细胞株PANC-1、SW1990中BANCR表达与MLVD的相关性分析
图3 BANCR对胰腺癌细胞VEGF-C、VEGFR-3 mRNA的影响 BANCR-siRNA为干扰BANCR表达后胰腺癌细胞SW1990;NC为未经siRNA干扰的NC组;*两组VEGF-C相比,P<0.05;#两组VEGFR-3相比,P<0.05
[1]
Chen W, Sun K, Zheng R, et al. Cancer incidence and mortality in China, 2014[J]. Chin J Cancer Res, 2018, 30(1): 1-12.
[2]
Kleeff J, Korc M, Apte M, et al. Pancreatic cancer[J]. Nat Rev Dis Primers, 2016, 2: 16022.
[3]
Schwarz RE, Smith DD. Extent of lymph node retrieval and pancreatic cancer survival: information from a large US population database[J]. Ann Surg Oncol, 2006, 13(9): 1189-1200.
[4]
Engreitz JM, Haines JE, Perez EM, et al. Local regulation of gene expression by lncRNA promoters, transcription and splicing[J]. Nature, 2016, 539(7629): 452-455.
[5]
Flockhart RJ, Webster DE, Qu K, et al. BRAFV600E remodels the melanocyte transcriptome and induces BANCR to regulate melanoma cell migration[J]. Genome Res, 2012, 22(6): 1006-1014.
[6]
Fang S, Liu Z, Guo Q, et al. High BANCR expression is associated with worse prognosis in human malignant carcinomas: An updated systematic review and Meta-analysis[J]. BMC Cancer, 2020, 20(1): 870.
[7]
Fan YH, Ye MH, Wu L, et al. BRAF-activated lncRNA predicts gastrointestinal cancer patient prognosis: A Meta-analysis[J]. Oncotarget, 2017, 8(4): 6295-6303.
[8]
Shen X, Bai Y, Luo B, et al. Upregulation of lncRNA BANCR associated with the lymph node metastasis and poor prognosis in colorectal cancer[J]. Biol Res, 2017, 50(1): 32.
[9]
Zhou T, Gao Y. Increased expression of LncRNA BANCR and its prognostic significance in human hepatocellular carcinoma[J]. World J Surg Oncol, 2016, 14(1): 8.
[10]
刘子豪,杨同昕,徐志鹏, 等. 长链非编码RNA BANCR在食管鳞癌中的表达及对细胞增殖和侵袭能力的影响[J]. 现代生物医学进展, 2016, 16(24): 4622-4627.
[11]
Lou KX, Li ZH, Wang P, et al. Long non-coding RNA BANCR indicates poor prognosis for breast cancer and promotes cell proliferation and invasion[J]. Eur Rev Med Pharmacol Sci, 2018, 22(5): 1358-1365.
[12]
Zheng H, Xu J, Hao S, et al. Expression of BANCR promotes papillary thyroid cancer by targeting thyroid stimulating hormone receptor[J]. Oncol Lett, 2018, 16(2): 2009-2015.
[13]
Wu X, Xia T, Cao M, et al. LncRNA BANCR promotes pancreatic cancer tumorigenesis via modulating miR-195-5p/Wnt/β-Catenin signaling pathway[J]. Technol Cancer Res Treat, 2019, 18: 1533033819887962.
[14]
Cheng P, Jin G, Hu X, et al. Analysis of tumor-induced lymphangiogenesis and lymphatic vessel invasion of pancreatic carcinoma in the peripheral nerve plexus[J]. Cancer Sci, 2012, 103(10): 1756-1763.
[15]
Keklikoglou I, Hosaka K, Bender C, et al. MicroRNA-206 functions as a pleiotropic modulator of cell proliferation, invasion and lymphangiogenesis in pancreatic adenocarcinoma by targeting ANXA2 and KRAS genes[J]. Oncogene, 2015, 34(37): 4867-4878.
[16]
Ochi N, Matsuo Y, Sawai H, et al. Vascular endothelial growth factor-C secreted by pancreatic cancer cell line promotes lymphatic endothelial cell migration in an in vitro model of tumor lymphangiogenesis[J]. Pancreas, 2007, 34(4): 444-451.
[1] 刘伟, 牛云峰, 安杰. LINC01232 通过miR-516a-5p/BCL9 轴促进三阴性乳腺癌的恶性进展[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 330-338.
[2] 罗文斌, 韩玮. 胰腺癌患者首次化疗后中重度骨髓抑制的相关危险因素分析及预测模型构建[J/OL]. 中华普通外科学文献(电子版), 2024, 18(05): 357-362.
[3] 马中正, 杨云川, 马翔, 周迟, 丁丁, 霍俊一, 徐楠, 崔培元, 周磊. 胰腺癌双硫死亡相关的lncRNA预后模型的构建及免疫反应研究[J/OL]. 中华普通外科学文献(电子版), 2024, 18(05): 368-376.
[4] 付成旺, 杨大刚, 王榕, 李福堂. 营养与炎症指标在可切除胰腺癌中的研究进展[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 704-708.
[5] 魏孔源, 仵正, 王铮, 黎韡. 机器人胰腺中段切除后远端胰腺消化道不同重建方式初探[J/OL]. 中华腔镜外科杂志(电子版), 2024, 17(05): 295-300.
[6] 郭诗翔, 谭明达, 王槐志. 胰头癌淋巴结清扫再思考[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 625-628.
[7] 张昊, 潘卫东. 胰腺癌新辅助化疗后可切除性评估现状及进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 629-633.
[8] 周倜, 吴嘉, 韩方, 徐林伟, 张宇华. 新辅助治疗时代胰腺癌淋巴结清扫研究进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 634-639.
[9] 王军华, 王锐炫. 胰腺癌新辅助化疗现状和治疗策略[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 640-643.
[10] 魏妙艳, 徐近. 合并远处转移胰腺癌系统性治疗的梳理和展望[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 644-650.
[11] 罗柳平, 吴萌萌, 陈欣磊, 林科灿. 胰腺全系膜切除在胰头癌根治术中的应用价值[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 651-656.
[12] 张瑜, 姜梦妮. 基于DWI信号值构建局部进展期胰腺癌放化疗生存获益预测模型[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 657-664.
[13] 王向前, 李清峰, 陈磊, 丘文丹, 姚志成, 李熠, 吴荣焕. 姜黄素抑制肝细胞癌索拉非尼耐药作用及其调控机制[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 729-735.
[14] 危用洋, 黄俊甫, 辛万鹏, 易思清, 涂书举, 方康, 李勇, 肖卫东. 三种术式治疗胰腺颈体部良性或低度恶性肿瘤的临床疗效分析[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(04): 515-519.
[15] 王国强, 张纲, 唐建坡, 张玉国, 杨永江. LINC00839 调节miR-17-5p/WEE1 轴对结直肠癌细胞增殖、凋亡和迁移的影响[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(06): 491-499.
阅读次数
全文
5
HTML PDF
最新录用 在线预览 正式出版 最新录用 在线预览 正式出版
0 0 1 0 0 4

  来源 本网站 其他网站
  次数 2 3
  比例 40% 60%

摘要
45
最新录用 在线预览 正式出版
0 0 45
  来源 本网站 其他网站
  次数 28 17
  比例 62% 38%