探讨P-糖蛋白（P-gp）、谷胱甘肽S转移酶（GST-π）和DNA拓扑异构酶Ⅱ（TopoⅡ）在胆道恶性肿瘤中的表达及其与临床病理参数的相关性,分析两者之间的关系。

采用免疫组化过氧化物酶标记链霉卵白素法(SP法）检测42例胆道恶性肿瘤患者组织中P-gp、GST-π和TopoⅡ的表达,分析其与肿瘤临床病理参数的关系及两者相关性。

P-gp、GST-π和TopoⅡ在胆道恶性肿瘤患者组织中的阳性表达率分别为88.1%、92.9%、73.8%。P-gp和GST-π的表达与患者的年龄、性别、肿瘤的分化程度、局部浸润、淋巴结转移均无明显关系；而TopoⅡ的表达与肿瘤的分化程度呈负相关性，随分化程度的降低而阳性表达率增高(P<0.05）；而与其他临床病理参数无相关性；P-gp和GST-π两者表达呈正相关(P<0.05）。而P-gp和TopoⅡ及GST-π和TopoⅡ之间均无显著相关性。

P-gp、GST-π和TopoⅡ在胆道恶性肿瘤中均有较高的表达水平，提示从未接受化疗的胆道恶性肿瘤患者存在较高的原发性耐药。联合检测这3个耐药蛋白的表达，对指导临床化疗有一定作用。

To explore the expression of P-gp, GST-π, and TopoⅡ in the malignant tumor of biliary tract and their relationship with the clinical pathological parameters.

The expression of P-gp, GST-π and TopoⅡ proteins in the 42 cases of the malignant tumors of biliary tract were tested by immunohistochemistry of peroxidase labeled streptavidin egg sensitized(SP). This work also made an aggregated analysis combining the P-gp, GST-π and TopoⅡ with the clinical pathological parameters and the mutual relationship between the three of them.

The positive expression rates of P-gp, GST-π and TopoⅡ in the malignant tumors of biliary tract were 88.1%, 92.9% and 73.8%. The expression of P-gp and GST-π weren't associated with the age, gender, degree of differentiation, the depth of invasion and lymph node metastasis. The expression of TopoⅡ wasn't associated with the clinical pathology factors except the degree of differentiation (P<0.05). There was a negative correlation between the expression of TopoⅡ and degree of differentiation. The expression of P-gp and GST-π had positive correlation(P<0.05). But there wasn't any correlation between the three index.

There are high expression of P-gp, GST-π and TopoⅡ in the malignant tumors of biliary tract, which hints that there is a high primary drug resistance phenomenon on the patients who haven't received any chemotherapy. It is of great significance of detecting aggregated expression of three drug resistance protein for the guiding clinical chemotherapy.