目的

探讨结直肠癌组织中血管内皮生长因子-C（VEGF-C）、酪氨酸激酶受体（Flt-4）及其存活素（Survivin）的表达与淋巴管生成、淋巴结转移的关系。

方法

采用酶组织化学方法及SP免疫组化法对86例结、直肠癌组织中VEGF-C、Flt-4和Survivin的表达及淋巴管的生成进行观察，结合病理报告中淋巴结转移的情况和肿瘤浸润深度，并与之比较。

结果

VEGF-C和Flt-4表达密切相关（r=0.981），VEGF-C阳性者淋巴管数较VEGF-C阴性者明显增多（27.20±6.36 vs 19.19±6.74）（P<0.01）。Flt-4阳性者淋巴管数较Flt-4阴性者明显增多（27.38±7.52 vs 20.17±7.14）（P<0.05），Survivin阳性淋巴管计数与Survivin阴性淋巴管计数无明显差异（22.15±6.92 vs 21.67±6.43）。但三者的表达均与淋巴结转移有关，VEGF-C的表达与肿瘤浸润的深度有关。

结论

Survivin影响VEGF-C的活性，VEGF-C可以激活间质中的Flt-4从而刺激淋巴管增生，为肿瘤细胞的淋巴结转移提供了必要的条件。

Objective

To investigate the expressions of VEGF-C,Fms-like Tyrosine kinase-4(Flt-4),Survivin and the relationships with lymphangiogenesis and metastases of lymph nodes in colorectal carcinoma tissues.

Methods

The expressions of VEGF-C,Flt-4 and Survivin were determined by immunohistochemistry (SP)and lymphatic vessles were stained by enzyme histochemical method in 86 cases of colorectal carcinomas.

Results

There was a significant correlation between the expressions of VEGF-C and Flt-4 in colorectal carcinoma (r=0.981).The numbers of lymphatic vessels in VEGF-C positive cases were significantly larger than that in VEGF-C negative cases (27.20±6.36 vs 19.19±6.74) (P<0.01).Similarly,the numbers of lymphatic vessels in Flt-4 positive tumors were larger than that in Flt-4 negative tumor(27.38±7.52vs 20.17±7.14)(P<0.05).There was no significant difference between the numbers of lymphatic vessels in Survivin positive and negative tumors (22.15±6.92vs 21.67±6.43).The expressions of VEGF-C,Flt-4 and Survivin were related to the metastases of lymphnodes.

Conclusion

Survivin may affect the activities of VEGF-C.VEGF-C may activate Flt-4 in mesenchyme and stimulate the proliferations of lymphatic vessels, which may provide necessary conditions for the metastasis of lymph nodes in colorectal carcinomas.