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中华普通外科学文献(电子版)

中华普通外科学文献(电子版) ›› 2011, Vol. 05 ›› Issue (01) : 21 -24. doi: 10.3877/cma.j.issn.1674-0793.2011.01.006

所属专题： 文献；

论著

光动力抑制胆管癌QBC939细胞生长的实验研究
曹良启1, 薛平1,†(), 陈育宾1, 邵子力1, 卢海武1, 郑强1, 温子龙1, 郑超1   
  1. 1. 510260　广州医学院第二附属医院肝胆外科
  • 收稿日期:2010-07-26 出版日期:2011-02-01
  • 通信作者: 薛平
  • 基金资助:
    广东省自然科学基金(9451018201003643); 广州医学院基金博士启动项目(2008C35); 广东省医学科学技术研究基金(A2007266)

Photodynamic therapy inhibits cell growth of human cholangiocarcinoma QBC939 cells in vitro

Liang-qi CAO1, Ping XUE1,†(), Yu-bin CHEN1, Zi-li SHAO1, Hai-wu LU1, Qiang ZHENG1, Zi-long WEN1, Chao ZHENG1   

  1. 1. Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
  • Received:2010-07-26 Published:2011-02-01
  • Corresponding author: Ping XUE
  • About author:
    Correspondening author: XUE Ping, Email:
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曹良启, 薛平, 陈育宾, 邵子力, 卢海武, 郑强, 温子龙, 郑超. 光动力抑制胆管癌QBC939细胞生长的实验研究[J]. 中华普通外科学文献(电子版), 2011, 05(01): 21-24.

Liang-qi CAO, Ping XUE, Yu-bin CHEN, Zi-li SHAO, Hai-wu LU, Qiang ZHENG, Zi-long WEN, Chao ZHENG. Photodynamic therapy inhibits cell growth of human cholangiocarcinoma QBC939 cells in vitro[J]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(01): 21-24.

目的

研究血卟啉衍生物介导的光动力（HPD-PDT）抑制胆管癌细胞生长状况及其机制。

方法

MTT法用于评估人胆管癌细胞（QBC939）的生长状态；Hoechst33258染色和流式细胞术检测细胞凋亡；Western Blotting法用于探测QBC939细胞中细胞色素C的释放情况；Caspases酶活性测定用于测定caspase-3、-8、-9的活化情况。

结果

HPD-PDT在体外能够抑制胆管癌QBC939细胞生长，这一效应主要是通过诱导QBC939细胞线粒体凋亡达到的，在凋亡过程中，出现了细胞色素C释放，caspase-9和-3的活化。

结论

HPD-PDT体外能够抑制胆管癌细胞生长，诱导胆管癌细胞凋亡。

关键词: 血卟啉衍生物, 光动力疗法, 凋亡, 胆管癌
Objective

To investigate the effects of hematoporphyrin derivative-mediated photodynamic therapy (HPD-PDT) on cell growth in human cholangiocarcinoma in vitro and in vivo, as well as the underlying mechanisms of these effects.

Methods

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate growth status of human cholangiocarcinoma cell line (QBC939). Hoechst 33258 staining and flow cytometry assays were applied to determine cell apoptosis. Western blotting analysis was performed to detect the release of cytochrome c in QBC939 cells, and caspases enzymatic assay was used to investigate the activation of caspase-3, -8, and -9.

Results

HPD-PDT inhibits QBC939 cell growth via cell apoptosis in vitro, and initiates cell mitochondria apoptosis pathway by the release of cytochrome c and the activation of caspase-9 and -3.

Conclusion

HPD-PDT inhibits tumor growth and induces cell apoptosis of human cholangiocarcinoma, suggesting that HPD-PDT is useful in cholangiocarcinoma therapy.

Key words: Hematoporphyrin derivative, Photodynamic therapy, Apoptosis, Cholangiocarcinoma
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