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中华普通外科学文献(电子版) ›› 2011, Vol. 05 ›› Issue (01) : 28 -34. doi: 10.3877/cma.j.issn.1674-0793.2011.01.008

所属专题: 文献

论著

性激素对烧伤血清处理后IEC-6细胞增殖与凋亡的影响
孙冠青1, 任政华2, 霍泽仁3, 杨婷1, 李盛1, 石汉平1,(), 管向东1   
  1. 1. 510080 广州,中山大学附属第一医院普通外科
    2. 中山大学生命科学学院
    3. 中山大学附属第二医院
  • 收稿日期:2010-03-12 出版日期:2011-02-01
  • 通信作者: 石汉平

Effects of gender hormones on proliferation and apoptosis of IEC-6 cells after cutaneous burn

Guan-qing SUN1, Zheng-hua REN2, Ze-ren HUO3, Ting YANG1, Sheng LI1, Han-ping SHI1,(), Xiang-dong GUAN1   

  1. 1. Department of General surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
  • Received:2010-03-12 Published:2011-02-01
  • Corresponding author: Han-ping SHI
  • About author:
    Corresponding author: SHI Han-ping, Email:
引用本文:

孙冠青, 任政华, 霍泽仁, 杨婷, 李盛, 石汉平, 管向东. 性激素对烧伤血清处理后IEC-6细胞增殖与凋亡的影响[J]. 中华普通外科学文献(电子版), 2011, 05(01): 28-34.

Guan-qing SUN, Zheng-hua REN, Ze-ren HUO, Ting YANG, Sheng LI, Han-ping SHI, Xiang-dong GUAN. Effects of gender hormones on proliferation and apoptosis of IEC-6 cells after cutaneous burn[J]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(01): 28-34.

目的

观察雌激素和雄激素对烧伤后小肠上皮细胞增殖和凋亡的影响。

方法

用30%体表面积Ⅲ度烫伤大鼠的血清处理大鼠源性IEC-6细胞,构建烧伤后小肠上皮的体外细胞模型。观察己烯雌酚或睾酮处理后的细胞增殖速率(MTT法和流式细胞仪检测)、细胞周期分布(流式细胞仪检测)、凋亡比率(DAPI染色法检测)、生长曲线(细胞分析仪检测)及Claudin-1表达(免疫组化法检测)。

结果

烧伤血清可促进IEC-6细胞增殖(0.777 vs 0.415,P<0.001),促细胞凋亡(10.5% vs 1.59%,P<0.001)和Claudin-1表达,减少IEC-6细胞总数。己烯雌酚和睾酮可同时抑制烧伤组细胞的增殖和凋亡,己烯雌酚处理后细胞的增殖指数(12.3% vs 14.6%;P<0.001)和凋亡比率(1.04% vs 2.33%,P<0.005)均低于睾酮处理后的细胞。

结论

烧伤后小肠上皮细胞凋亡速度超过了增殖速度,己烯雌酚和睾酮可同时抑制烧伤后小肠上皮细胞的增殖和凋亡,其中己烯雌酚的作用较睾酮更明显。

Objective

To explore the effects of sex steroids on post-burn intestinal epithelial cells.

Methods

Sera from SD rats which received 30% total-body-surface full-thickness burn or sham treatment were used to treat confluent monolayer of IEC-6 cells. Diethylstilbestrol (DES), testosterone (T), or vehicle were then added. the effects on proliferation, cell-cycle distribution, apoptosis, cell number and Claudin-1 expression were investigated by means of MTT assays, flow cytometry, DAPI staining, RT-CES and fluorescent immunoassay, respectively.

Results

Serum from burned animals enhanced proliferation (0.777 vs 0.415, P<0.001), apoptosis (10.5% vs 1.59%, P<0.001) and Claudin-1 expression of IEC-6 cells, while reduced the cell number. Both DES and T inhibited proliferation and apoptosis of cells treated by scald serum. Specifically, proliferation index (12.3% vs 14.6%, P<0.001) and apoptotic rate (1.04% vs 2.33%, P<0.005) were lower in cells treated by DES than cells treated by T.

Conclusions

Post-burn intestinal epithelial barrier dysfunction is mainly due to surpass of apoptosis over proliferation. Both gender hormones inhibit proliferation and apoptosis in intestinal epithelial cells caused by cutaneous burn. In addition, estrogen had more obvious effects than androgen.

图1 IEC-6细胞形态学改变
图2 各组细胞的增殖速率(MTT法)
图3 烧伤组与对照组细胞的S期比率和增殖指数(流式细胞仪法)
图4 烧伤血清处理组与对照组细胞的凋亡速率比较(DAPI法)
图5 对照组IEC-6细胞(a)和1:100倍稀释烧伤血清处理的细胞(b)生长曲线比较(RT-CES法)
图6 各组IEC-6细胞的Claudin-1的表达
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