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中华普通外科学文献(电子版) ›› 2011, Vol. 05 ›› Issue (01) : 28 -34. doi: 10.3877/cma.j.issn.1674-0793.2011.01.008

所属专题: 文献

论著

性激素对烧伤血清处理后IEC-6细胞增殖与凋亡的影响
孙冠青1, 任政华2, 霍泽仁3, 杨婷1, 李盛1, 石汉平1,(), 管向东1   
  1. 1. 510080 广州,中山大学附属第一医院普通外科
    2. 中山大学生命科学学院
    3. 中山大学附属第二医院
  • 收稿日期:2010-03-12 出版日期:2011-02-01
  • 通信作者: 石汉平

Effects of gender hormones on proliferation and apoptosis of IEC-6 cells after cutaneous burn

Guan-qing SUN1, Zheng-hua REN2, Ze-ren HUO3, Ting YANG1, Sheng LI1, Han-ping SHI1,(), Xiang-dong GUAN1   

  1. 1. Department of General surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
  • Received:2010-03-12 Published:2011-02-01
  • Corresponding author: Han-ping SHI
  • About author:
    Corresponding author: SHI Han-ping, Email:
引用本文:

孙冠青, 任政华, 霍泽仁, 杨婷, 李盛, 石汉平, 管向东. 性激素对烧伤血清处理后IEC-6细胞增殖与凋亡的影响[J/OL]. 中华普通外科学文献(电子版), 2011, 05(01): 28-34.

Guan-qing SUN, Zheng-hua REN, Ze-ren HUO, Ting YANG, Sheng LI, Han-ping SHI, Xiang-dong GUAN. Effects of gender hormones on proliferation and apoptosis of IEC-6 cells after cutaneous burn[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(01): 28-34.

目的

观察雌激素和雄激素对烧伤后小肠上皮细胞增殖和凋亡的影响。

方法

用30%体表面积Ⅲ度烫伤大鼠的血清处理大鼠源性IEC-6细胞,构建烧伤后小肠上皮的体外细胞模型。观察己烯雌酚或睾酮处理后的细胞增殖速率(MTT法和流式细胞仪检测)、细胞周期分布(流式细胞仪检测)、凋亡比率(DAPI染色法检测)、生长曲线(细胞分析仪检测)及Claudin-1表达(免疫组化法检测)。

结果

烧伤血清可促进IEC-6细胞增殖(0.777 vs 0.415,P<0.001),促细胞凋亡(10.5% vs 1.59%,P<0.001)和Claudin-1表达,减少IEC-6细胞总数。己烯雌酚和睾酮可同时抑制烧伤组细胞的增殖和凋亡,己烯雌酚处理后细胞的增殖指数(12.3% vs 14.6%;P<0.001)和凋亡比率(1.04% vs 2.33%,P<0.005)均低于睾酮处理后的细胞。

结论

烧伤后小肠上皮细胞凋亡速度超过了增殖速度,己烯雌酚和睾酮可同时抑制烧伤后小肠上皮细胞的增殖和凋亡,其中己烯雌酚的作用较睾酮更明显。

Objective

To explore the effects of sex steroids on post-burn intestinal epithelial cells.

Methods

Sera from SD rats which received 30% total-body-surface full-thickness burn or sham treatment were used to treat confluent monolayer of IEC-6 cells. Diethylstilbestrol (DES), testosterone (T), or vehicle were then added. the effects on proliferation, cell-cycle distribution, apoptosis, cell number and Claudin-1 expression were investigated by means of MTT assays, flow cytometry, DAPI staining, RT-CES and fluorescent immunoassay, respectively.

Results

Serum from burned animals enhanced proliferation (0.777 vs 0.415, P<0.001), apoptosis (10.5% vs 1.59%, P<0.001) and Claudin-1 expression of IEC-6 cells, while reduced the cell number. Both DES and T inhibited proliferation and apoptosis of cells treated by scald serum. Specifically, proliferation index (12.3% vs 14.6%, P<0.001) and apoptotic rate (1.04% vs 2.33%, P<0.005) were lower in cells treated by DES than cells treated by T.

Conclusions

Post-burn intestinal epithelial barrier dysfunction is mainly due to surpass of apoptosis over proliferation. Both gender hormones inhibit proliferation and apoptosis in intestinal epithelial cells caused by cutaneous burn. In addition, estrogen had more obvious effects than androgen.

图1 IEC-6细胞形态学改变
图2 各组细胞的增殖速率(MTT法)
图3 烧伤组与对照组细胞的S期比率和增殖指数(流式细胞仪法)
图4 烧伤血清处理组与对照组细胞的凋亡速率比较(DAPI法)
图5 对照组IEC-6细胞(a)和1:100倍稀释烧伤血清处理的细胞(b)生长曲线比较(RT-CES法)
图6 各组IEC-6细胞的Claudin-1的表达
1
Choudhry MA, Rana SN, Kavanaugh MJ, et al. Impaired intestinal immunity and barrier function: a cause for enhanced bacterial translocation in alcohol intoxication and burn injury. Alcohol, 2004, 33(3): 199-208.
2
Al-Ghoul WM, Khan M, Fazal N, et al. Mechanisms of postburn intestinal barrier dysfunction in the rat: roles of epithelial cell renewal, E-cadherin, and neutrophil extravasation. Crit Care Med, 2004, 32(8): 1730-1739.
3
宋国栋, 王德昌, 贾军, 等. 重组人生长激素对烧伤大鼠肠粘膜结构及细胞凋亡的影响. 中华烧伤杂志, 2002, 18(4): 207-209.
4
赵云, 王凤君, 王裴, 等. 胰高血糖素样肽-2对烧伤大鼠肠粘膜细胞增殖的影响. 中华烧伤杂志, 2003, 19(4): 209-212.
5
Varedi M, Greeley GH, Herndon DN, et al. A thermal injury-induced circulating factor(s) compromises intestinal cell morphology, proliferation, and migration. Am J Physiol Gastrointest Liver Physiol, 1999, 277(1): G175-182.
6
Kovacs EJ. Aging, traumatic injury, and estrogen treatment. Exp Gerontol, 2005, 40(7): 549-555.
7
Horton JW, White DJ, Maass DL. Gender-related differences in myocardial inflammatory and contractile responses to major burn trauma. Am J Physiol Heart Circ Physiol, 2004, 286(1): H202-213.
8
Ozveri ES, Bozkurt A, Haklar G, et al. Estrogens ameliorate remote organ inflammation induced by burn injury in rats. Inflamm Res, 2001, 50(12):585-591.
9
Di Domenico M, Castoria G, Bilancio A, et al. Estradiol activation of human colon carcinoma-derived Caco-2 cell growth. Cancer Res, 1996, 56(19):4516-4521.
10
Booth C, Hargreaves DF, Hadfield JA, et al. Isoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro. Br J Cancer, 1999, 80(10): 1550-1557.
11
Wright NA, Morley AR. The effect of testosterone on the growth fraction of the mouse small intestine. J Endocrinol, 1971, 50(2): 351-352.
12
Quaroni A, Wands J, Trelstad RL, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J Cell Biol, 1979, 80(2): 248-265.
13
Spies M, Chappell VL, Dasu MR, et al. Role of TNF-α in gut mucosal changes after severe burn. Am J Physiol Gastrointest Liver Physiol, 2002, 283(4): G703-708.
14
Wolf SE, Ikeda H, Matin S, et al. Cutaneous burn increases apoptosis in the gut epithelium of mice. J Am Coll Surg, 1999, 188(1): 10-16.
15
Huang Y, Zheng J, Fan P, et al. Transfection of antisense p38 alpha gene ameliorates myocardial cell injury mediated by hypoxia and burn serum. Burns, 2007, 33(5): 599-605.
16
Wu X, Woodside KJ, Song J, et al. Burn-induced gut mucosal homeostasis in TCR delta receptor-deficient mice. Shock, 2004, 21(1): 52-57.
17
Abreu MT, Arnold ET, Chow JY, et al. Phosphatidylinositol 3-kinase-dependent pathways oppose Fas-induced apoptosis and limit chloride secretion in human intestinal epithelial cells. Implications for inflammatory diarrheal states. J Biol Chem, 2001, 276(50): 47 563-47 574.
18
Jeschke MG, Bolder U, Chung DH, et al. Gut mucosal homeostasis and cellular mediators after severe thermal trauma and the effect of insulin-like growth factor-I in combination with insulin-like growth factor binding protein-3. Endocrinology, 2007, 148(1): 354-362.
19
Yu HP, Hsieh YC, Suzuki T, et al. Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway. J Leukoc Biol, 2007, 82(1): 1-7.
20
Krajewski S, Krajewska M, Shabaik A, et al. Immunohistochemical determination of In vivo distribution of bax, a dominant inhibitor of bcI-2. Am J Pathol, 1994, 145(6): 1323-1336.
21
Shima Y, Tajiri T, Taguchi T, et al. Increased expression of c-fos and c-jun in the rat small intestinal epithelium after ischemia-reperfusion injury: a possible correlation with the proliferation or apoptosis of intestinal epithelial cells. J Pediatr Surg, 2006, 41(4): 830-836.
22
Song J, Wolf SE, Herndon DN, et al. Second hit post burn increased proximal gut mucosa epithelial cells damage. Shock, 2008, 30(2): 184-188.
23
Saegusa M, Takano Y, Okayasu I. Bcl-2 expression and its association with cell kinetics in human gastric carcinomas and intestinal metaplasia. J Cancer Res Clin Oncol, 1995, 121(6): 357-363.
24
Clark J, Saunders T, Doelle S, et al. Tight junctions formation in experimental model of necrotizing enterocolitis-effect of EGF treatment. J Pediatr Gastroenterol Nutr, 2005, 40(5): 617.
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