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中华普通外科学文献(电子版)

中华普通外科学文献(电子版) ›› 2015, Vol. 09 ›› Issue (05) : 351 -355. doi: 10.3877/cma.j.issn.1674-0793.2015.05.006

所属专题： 文献；

论著

15-脱氧-前列腺素J2激活过氧化物酶体增殖物激活受体信号抑制肝癌细胞侵袭转移的实验研究
曹良启1,†(), 王闯1, 薛平1, 张大伟1, 梁惠宏1, 邵子力1   
  1. 1. 510260　广州医科大学附属第二医院肝胆外科
  • 收稿日期:2015-03-29 出版日期:2015-10-01
  • 通信作者: 曹良启
  • 基金资助:
    广东省科技计划项目(2014A020212326); 广州市科技划项目-珠江科技新星专项(2012J2200039)

PPARγ signal pathway initiated by 15-deoxy-prostaglandin J2 suppressing the invasion and metastasis of hepatocellular carcinoma

Liangqi Cao1,†(), Chuang Wang1, Ping Xue1, Dawei Zhang1, Huihong Liang1, Zili Shao1   

  1. 1. Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
  • Received:2015-03-29 Published:2015-10-01
  • Corresponding author: Liangqi Cao
  • About author:
    Corresponding author: Cao Liangqi, Email:
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曹良启, 王闯, 薛平, 张大伟, 梁惠宏, 邵子力. 15-脱氧-前列腺素J2激活过氧化物酶体增殖物激活受体信号抑制肝癌细胞侵袭转移的实验研究[J]. 中华普通外科学文献(电子版), 2015, 09(05): 351-355.

Liangqi Cao, Chuang Wang, Ping Xue, Dawei Zhang, Huihong Liang, Zili Shao. PPARγ signal pathway initiated by 15-deoxy-prostaglandin J2 suppressing the invasion and metastasis of hepatocellular carcinoma[J]. Chinese Archives of General Surgery(Electronic Edition), 2015, 09(05): 351-355.

目的

探讨15-脱氧-前列腺素J2(15-d-PGJ2)对肝癌细胞SMMC-7721侵袭转移的影响及其内在的机制。

方法

SMMC-7721肝癌细胞经天然配体15-d-PGJ2和(或)抑制剂GW9662处理,用伤口愈合实验和Transwell方法检测细胞的转移侵袭能力,用RT-PCR检测细胞PTEN和MMP-9 mRNA的表达,用Western blotting检测细胞PTEN和MMP-9蛋白的表达。

结果

15-d-PGJ2对SMMC-7721细胞的转移侵袭具有抑制作用,并能上调PTEN mRNA和蛋白的表达,下调MMP-9 mRNA和蛋白的表达;单药GW9662无此效应,GW9662与15-d-PGJ2联合能够逆转此效应。

结论

15-d-PGJ2是通过激活PPAR信号上调PTEN表达,下调MMP-9表达,抑制肝癌细胞的侵袭转移。

关键词: 过氧化物酶体增殖物激活受体, 肝细胞癌, PTEN, 侵袭转移
Objective

To investigate the effect of 15-deoxy-prostaglandin J2 (15-d-PGJ2) on the invasion and metastasis of hepatocellular carcinoma SMMC-7721 cells, as well as its underlying mechanisms.

Methods

Hepatocellular carcinoma SMMC-7721 cells were treated by natural ligand 15-d-PGJ2 with or without antagonist GW9662 of peroxisome proliferator-activated receptor (PPAR). Invasion and metastasis of SMMC-7721 cells was measured by wound healing assay and Transwell chambers. mRNAs of PTEN and MMP-9 were assessed by RT-PCR and proteins by Western Blotting analysis, respectively.

Results

15-d-PGJ2, but not GW9662, inhibited the invasion and metastasis of SMMC-7721 cells, up-regulated PTEN mRNA and protein expression, and down-regulated MMP-9 mRNA and protein expression. However, after SMMC-7721 cells were pretreated for 1 h with GW9662 and exposed to 15-d-PGJ2, the inhibitive effects of 15-d-PGJ2 on invasion and metastasis of cancer cells were reversed.

Conclusion

15-d-PGJ2 increases PTEN expression and decreases MMP-9 expression to suppress the invasion and metastasis of HCC via activating PPAR signal pathway.

Key words: Peroxisome proliferator activated receptor, Hepatocellular carcinoma, PTEN, Invasion and metastasis
图1 15-d-PGJ2和GW9662对SMMC-7721细胞迁移的影响
图2 15-d-PGJ2和GW9662对SMMC-7721细胞侵袭的影响(×200)
图3 RT-PCR结果显示PTEN mRNA和MMP-9 mRNA相对表达量
图4 Western Blotting结果显示PTEN和MMP-9的表达
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