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中华普通外科学文献(电子版) ›› 2016, Vol. 10 ›› Issue (03) : 179 -182. doi: 10.3877/cma.j.issn.1674-0793.2016.03.004

所属专题: 文献

论著

生物钟基因CLOCK蛋白对甲状腺乳头状癌的影响
韩昌1, 刘冬良1, 周峰1, 张召辉,1, 李德本2, 牛万成1   
  1. 1. 221004 徐州,蚌埠医学院附属解放军第九七医院普外科
    2. 221004 徐州,蚌埠医学院病理科
  • 收稿日期:2016-01-22 出版日期:2016-06-01
  • 通信作者: 张召辉
  • 基金资助:
    国家自然科学基金资助项目(81201583)

Significance of circadian genes clock protein in papillary thyroid carcinoma tissues

Chang Han1, Dongliang Liu1, Feng Zhou1, Zhaohui Zhang,1, Deben Li2, Wancheng Niu1   

  1. 1. Department of General Surgery, Affiliated PLA No. 97 Hospital of Bengbu Medical College, Xuzhou 221004, China
    2. Department of Pathology, Affiliated PLA No. 97 Hospital of Bengbu Medical College, Xuzhou 221004, China
  • Received:2016-01-22 Published:2016-06-01
  • Corresponding author: Zhaohui Zhang
  • About author:
    Corresponding author: Zhang Zhaohui, Email:
引用本文:

韩昌, 刘冬良, 周峰, 张召辉, 李德本, 牛万成. 生物钟基因CLOCK蛋白对甲状腺乳头状癌的影响[J/OL]. 中华普通外科学文献(电子版), 2016, 10(03): 179-182.

Chang Han, Dongliang Liu, Feng Zhou, Zhaohui Zhang, Deben Li, Wancheng Niu. Significance of circadian genes clock protein in papillary thyroid carcinoma tissues[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2016, 10(03): 179-182.

目的

探讨生物钟基因CLOCK在甲状腺乳头状癌组织中的蛋白表达,并分析其与甲状腺癌病理特征的关系。

方法

收集2012年3月到2015年3月在中国人民解放军第九七医院手术切除的甲状腺乳头状癌标本74份,采用免疫组化方法检测CLOCK蛋白在甲状腺乳头状癌及配对癌旁组织中的表达水平,分析其表达与临床病理特征的关系。

结果

甲状腺乳头状癌组织与其配对的癌旁正常组织中CLOCK蛋白阳性表达分别为68.9%(51/74)、20.3%(15/74),两类组织中阳性率的差异有统计学意义(χ2=35.44,P=0.000)。CLOCK蛋白表达情况与甲状腺乳头状癌淋巴结转移情况、肿瘤直径及TNM分期有关(P <0.05)。

结论

CLOCK蛋白可能与甲状腺乳头状癌的发生、肿瘤的侵袭和转移等关系密切,可为甲状腺癌的早期诊断提供依据。

Objective

To investigate the protein expression level of CLOCK gene in papillary thyroid carcinoma (PTC) tissues and the relationship between the expression level and pathological features of patients with PTC.

Methods

PTC patients undergoing radical surgery at Affliated PLA No. 97 Hospital from March 2012 to March 2015 were collected. Finally, seventy four cases were enrolled in this study. The expression level of CLOCK protein was analyzed by immunohistochemistry in PTC tissues and the paired adjacent normal thyroid tissues. The relationship between the expression level of CLOCK protein and clinicopathological features were also analyzed.

Results

The positive expression rates of CLOCK protein in PTC tissues and paired adjacent normal tissues were 68.9% (51/74) and 20.3% (15/74), respectively, with significant difference (χ2=35.44, P=0.000). The expression level of CLCOK protein in thyroid cancer was related to lymph node metastasis, diameter of tumor and TNM stage.

Conclusion

The CLOCK protein plays an important role in tumorigenesis, tumor invasion and metastasis.

表1 CLOCK蛋白在甲状腺癌及正常甲状腺组织中的表达(n=74)
图1 SP法检测CLOCK基因在癌组织和正常组织中的蛋白表达情况(DAB ×200)A:CLOCK基因在甲状腺癌组织中的强阳性表达;B:CLOCK基因在正常甲状腺组织中的阳性表达
表2 CLOCK蛋白的表达与甲状腺乳头状癌临床病理特征的关系(例)
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