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中华普通外科学文献(电子版) ›› 2017, Vol. 11 ›› Issue (03) : 154 -158. doi: 10.3877/cma.j.issn.1674-0793.2017.03.003

所属专题: 文献

论著

GAS5来源的piRNAs上调乳腺癌细胞中FOXO4基因的表达研究
陈欣欣1, 夏婷1, 胡小戊1, 曹腾飞1, 章乐虹1,()   
  1. 1. 510260 广州医科大学附属第二医院乳腺外科
  • 收稿日期:2016-11-06 出版日期:2017-06-01
  • 通信作者: 章乐虹
  • 基金资助:
    广东省教育厅青年创新人才类项目基金资助项目(2015KQNCX124)

piRNAs derived from GAS5 activating tumor suppressor FOXO4 in breast cancer cell

Xinxin Chen1, Ting Xia1, Xiaowu Hu1, Tengfei Cao1, Lehong Zhang1,()   

  1. 1. Department of Breast Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510080, China
  • Received:2016-11-06 Published:2017-06-01
  • Corresponding author: Lehong Zhang
  • About author:
    Corresponding author: Zhang Lehong, Email:
引用本文:

陈欣欣, 夏婷, 胡小戊, 曹腾飞, 章乐虹. GAS5来源的piRNAs上调乳腺癌细胞中FOXO4基因的表达研究[J/OL]. 中华普通外科学文献(电子版), 2017, 11(03): 154-158.

Xinxin Chen, Ting Xia, Xiaowu Hu, Tengfei Cao, Lehong Zhang. piRNAs derived from GAS5 activating tumor suppressor FOXO4 in breast cancer cell[J/OL]. Chinese Archives of General Surgery(Electronic Edition), 2017, 11(03): 154-158.

目的

探讨GAS5来源的piRNAs在乳腺癌细胞中对FOXO4基因的影响。

方法

将GAS5来源的5个pi-snoRNAs(pi-sno44/74/75/78/81)转入乳腺癌细胞后收样提取总RNA行mRNA芯片检测,探索受到上述5个piRNAs调节的基因;分别将5个piRNAs转入乳腺癌细胞中检测每个piRNA对FOXO4 mRNA水平及蛋白水平的表达的影响。收集29例乳腺癌及癌旁组织样本提取总RNA检测FOXO4的表达情况;应用关联分析对pi-sno44/74/75/78/81及FOXO4在乳腺癌及癌旁乳腺组织中的表达情况进行相关性分析,了解piRNAs与FOXO4的相关性。

结果

mRNA芯片提示FOXO4基因的表达被明显上调;其中pi-sno44/74/75/81能够上调FOXO4的表达,而pi-sno78对FOXO4激活的作用不明显;pi-sno44/74/75/81在乳腺癌及癌旁乳腺组织中的表达与FOXO4在乳腺癌及癌旁乳腺组织中的表达成正相关(Corr=0.64、0.91、0.76、0.93,P<0.001),pi-sno78的表达与FOXO4的表达无明显相关性(Corr=0.25,P=0.912)。

结论

GAS5来源的pi-sno44/74/75/81能上调FOXO4基因的表达,并可能通过影响FOXO4的表达影响乳腺癌细胞的生长。

Objective

To investigate the activation of FOXO4 by piRNAs derived from GAS5 in breast cancer cell.

Methods

A mixture of the five chemically synthesized single-stranded piRNAs, including pi-sno44/74/75/78/81, were transfected into MCF7 cells. Protein expression of FOXO4 and gene expression detected by microarray mRNA, and quantitative analysis of FOXO4 was performed from 29 clinical breast samples.

Results

FOXO4 expression was up-regulated in breast cancer cells, and the up-regulation was related to pi-sno44/74/75/81 (Corr=0.64, 0.91, 0.76, 0.93, P<0.001), but not to pi-sno78 (Corr=0.25, P=0.912).

Conclusion

piRNAs including pi-sno44/74/75/81 derived from GAS5 can mediate the induction of FOXO4, and may lead to the inhibition of tumor growth.

图1 mRNA芯片检测发现GAS5来源的piRNAs上调了FOXO4的表达 M-piRNA代表转染了5个pi-snoRNAs的MCF-7细胞,M-mock代表对照
图2 pi-sno44/74/75/78/81对乳腺癌细胞中FOXO4基因表达的影响
图3 FOXO4在乳腺癌组织中的低表达
图4 pi-sno44/74/75/78/81和GAS5与FOXO4在乳腺癌及癌旁乳腺组织中表达情况的相关性
图5 FOXO4与TRAIL在乳腺癌及癌旁乳腺组织中表达情况的相关分析
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