白细胞介素2基因转染对裸鼠乳腺癌肺转移的影响

doi:10.3877/cma.j.issn.1674-0793.2017.03.005

中华普通外科学文献(电子版) ›› 2017, Vol. 11 ›› Issue (03) : 164 -167. doi: 10.3877/cma.j.issn.1674-0793.2017.03.005

所属专题：文献；

论著

白细胞介素2基因转染对裸鼠乳腺癌肺转移的影响

赵广峰¹, 朱易凡²^,^†(

)

^1. 510170　广州医科大学附属第三医院荔湾医院普通外科
^2. 510080　广州，中山大学附属第一医院甲状腺乳腺外科

收稿日期:2016-11-04 出版日期:2017-06-01
通信作者: 朱易凡

Effect of interleukin-2 gene transfection on pulmonary metastasis of breast cancer in nude mice

Guangfeng Zhao¹, Yifan Zhu²^,^†()

^1. Department of General Surgery, Liwan Hospital of The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510170, China
^2. Department of Thyroid and Breast Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China

Received:2016-11-04 Published:2017-06-01
Corresponding author: Yifan Zhu
About author:
Corresponding author: Zhu Yifan, Email: zyf0924@126.com

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目的

观察白细胞介素2（IL-2）基因转染治疗裸鼠乳腺癌肺转移的疗效。

方法

在乳腺癌细胞株MD231和BT474中转染pORF-hIL-2质粒，检测对迁移和侵袭能力的影响，同时制作裸鼠乳腺癌肺转移模型30只，随机分为pORF-hIL-2质粒转染实验组和对照组，以瘤内注射的方式将脂质体和pORF-hIL-2质粒混合液注射入皮下瘤体内，观察肿瘤体积变化以及肺部转移病灶。

结果

pORF-hIL-2质粒转入乳腺癌细胞株后，两组细胞均出现迁移和侵袭能力的下降；经治疗后对比，实验组模型的皮下结节肿瘤体积增长明显受抑制，差异有统计学意义（t=-8.214，P<0.001）；两组观察期实验后肺部转移灶的数目差异亦有统计学意义（t=3.854，P=0.007）。

结论

应用IL-2基因质粒瘤内注射方法治疗乳腺癌伴肺转移的裸鼠模型可以产生抗瘤效应，而且能有效抑制肺部的转移病灶。

关键词: 乳腺肿瘤, 基因疗法, 转染

Objective

To evaluate the effect of interleukin-2 (IL-2) gene transfection on treating pulmonary metastasis of breast cancer in nude mice.

Methods

Breast cancer lines MD231 and BT474 were first transfected with pORF-hIL-2 plasmid, then cell invasiveness and migration capability were tested. Thirty nude mice with pulmonary metastasis of breast cancer were randomly divided into pORF-hIL-2 plasmid transfection group and the control group, then the mixture of liposome and pORF-hIL-2 plasmid was injected into their tumors subcutaneously, and finally the volumetric change of the tumors and the lung metastic lesions was observed.

Results

The cell invasiveness and migration capability diminished in MD231 and BT474 after transfected with pORF-hIL-2 plasmid. Compared with the control group, the volumetric growth of the subcutaneous nodular tumors in the experiment group were inhibited obviously, and the difference was statistically significant (t=-8.214, P<0.001); after the study period, the difference of the number of the lung metastic lesions in the two groups were statistically significant (t=3.854, P=0.007).

Conclusion

IL-2 gene plasmid intratumoral injection has shown to improve anti-tumor effects significantly to treat pulmonary metastasis of breast cancer in nude mice, and to inhibit lung metastatic lesions effectively.

Key words: Breast neoplasms, Gene therapy, Transfection

图1 pORF-hIL-2质粒转入MD231和BT474细胞株后，两种细胞株出现的迁移和侵袭能力的下降

图2 pORF-hIL-2质粒转染组与空白对照组裸鼠肿瘤体积对照^*P<0.05,^**P<0.01,^***P<0.001

图3 pORF-hIL-2质粒转染组与空白对照组裸鼠肺转移瘤数目对照^**P=0.007

[1]	Li S, Xu X, Jiang M, et al. Lipopolysaccharide induces inflammation and facilitates lung metastasis in a breast cancer model via the prostaglandin E2-EP2 pathway[J]. Mol Med Rep, 2015, 11(6):4454-4462.
[2]	Coffelt SB, Kersten K, Doornebal CW, et al. IL-17-producing γδ T cells and neutrophils conspire to promote breast cancer metastasis[J]. Nature, 2015, 522(7556):345-348.
[3]	Bonini C, Parmiani G. When transgenes shape immunity: cancer immune-gene therapy[J]. J Gene Med, 2012, 14(6):384-385.
[4]	叶小鸣，左继东，朱易凡. IL-2基因转染联合CTL杀伤肝癌细胞的体外试验研究[J]. 中国实用外科杂志, 2006, 26(9):689-690.
[5]	朱易凡，叶小鸣，左继东, 等. 白细胞介素-2基因转染联合特异性细胞毒性T淋巴细胞对裸鼠肝癌的影响[J]. 中华实验外科杂志, 2007, 24(12):1508-1509.
[6]	Harbeck N, Gnant M. Breast cancer[J]. Lancet, 2016, S0140-6736(16):31891-31898.
[7]	Nagata C, Wada K, Tsuji M, et al. Plasma amino acid profiles are associated with biomarkers of breast cancer risk in premenopausal Japanese women[J]. Cancer Causes Control, 2014, 25(2):143-149.
[8]	李雯雯，赵广银，张兆, 等. ¹⁸F-FDG PET/CT成像观察西妥昔单抗对结直肠癌裸鼠移植肿瘤影响的实验研究[J/CD]. 中华普通外科学文献(电子版), 2015, 9(3):188-192.
[9]	曹大勇，杨静悦，窦科峰. 白细胞介素-2基因修饰树突状细胞体外增强其诱导的抗肝癌免疫反应[J]. 中华实验外科杂志, 2007, 24(3):285-287.
[10]	Kaufman HL, Flanagan K, Lee CS, et al. Insertion of interleukin-2 (IL-2) and interleukin-12 (IL-12) genes into vaccinia virus results in effective anti-tumor responses without toxicity[J]. Vaccine, 2002, 20(13-14):1862-1869.

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