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中华普通外科学文献(电子版)

中华普通外科学文献(电子版) ›› 2017, Vol. 11 ›› Issue (03) : 164 -167. doi: 10.3877/cma.j.issn.1674-0793.2017.03.005

所属专题： 文献；

论著

白细胞介素2基因转染对裸鼠乳腺癌肺转移的影响
赵广峰1, 朱易凡2,†()   
  1. 1. 510170　广州医科大学附属第三医院荔湾医院普通外科
    2. 510080　广州，中山大学附属第一医院甲状腺乳腺外科
  • 收稿日期:2016-11-04 出版日期:2017-06-01
  • 通信作者: 朱易凡

Effect of interleukin-2 gene transfection on pulmonary metastasis of breast cancer in nude mice

Guangfeng Zhao1, Yifan Zhu2,†()   

  1. 1. Department of General Surgery, Liwan Hospital of The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510170, China
    2. Department of Thyroid and Breast Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
  • Received:2016-11-04 Published:2017-06-01
  • Corresponding author: Yifan Zhu
  • About author:
    Corresponding author: Zhu Yifan, Email:
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引用本文：

赵广峰, 朱易凡. 白细胞介素2基因转染对裸鼠乳腺癌肺转移的影响[J]. 中华普通外科学文献(电子版), 2017, 11(03): 164-167.

Guangfeng Zhao, Yifan Zhu. Effect of interleukin-2 gene transfection on pulmonary metastasis of breast cancer in nude mice[J]. Chinese Archives of General Surgery(Electronic Edition), 2017, 11(03): 164-167.

目的

观察白细胞介素2（IL-2）基因转染治疗裸鼠乳腺癌肺转移的疗效。

方法

在乳腺癌细胞株MD231和BT474中转染pORF-hIL-2质粒，检测对迁移和侵袭能力的影响，同时制作裸鼠乳腺癌肺转移模型30只，随机分为pORF-hIL-2质粒转染实验组和对照组，以瘤内注射的方式将脂质体和pORF-hIL-2质粒混合液注射入皮下瘤体内，观察肿瘤体积变化以及肺部转移病灶。

结果

pORF-hIL-2质粒转入乳腺癌细胞株后，两组细胞均出现迁移和侵袭能力的下降；经治疗后对比，实验组模型的皮下结节肿瘤体积增长明显受抑制，差异有统计学意义（t=-8.214，P<0.001）；两组观察期实验后肺部转移灶的数目差异亦有统计学意义（t=3.854，P=0.007）。

结论

应用IL-2基因质粒瘤内注射方法治疗乳腺癌伴肺转移的裸鼠模型可以产生抗瘤效应，而且能有效抑制肺部的转移病灶。

关键词: 乳腺肿瘤, 基因疗法, 转染
Objective

To evaluate the effect of interleukin-2 (IL-2) gene transfection on treating pulmonary metastasis of breast cancer in nude mice.

Methods

Breast cancer lines MD231 and BT474 were first transfected with pORF-hIL-2 plasmid, then cell invasiveness and migration capability were tested. Thirty nude mice with pulmonary metastasis of breast cancer were randomly divided into pORF-hIL-2 plasmid transfection group and the control group, then the mixture of liposome and pORF-hIL-2 plasmid was injected into their tumors subcutaneously, and finally the volumetric change of the tumors and the lung metastic lesions was observed.

Results

The cell invasiveness and migration capability diminished in MD231 and BT474 after transfected with pORF-hIL-2 plasmid. Compared with the control group, the volumetric growth of the subcutaneous nodular tumors in the experiment group were inhibited obviously, and the difference was statistically significant (t=-8.214, P<0.001); after the study period, the difference of the number of the lung metastic lesions in the two groups were statistically significant (t=3.854, P=0.007).

Conclusion

IL-2 gene plasmid intratumoral injection has shown to improve anti-tumor effects significantly to treat pulmonary metastasis of breast cancer in nude mice, and to inhibit lung metastatic lesions effectively.

Key words: Breast neoplasms, Gene therapy, Transfection
图1 pORF-hIL-2质粒转入MD231和BT474细胞株后，两种细胞株出现的迁移和侵袭能力的下降
图2 pORF-hIL-2质粒转染组与空白对照组裸鼠肿瘤体积对照*P<0.05,**P<0.01,***P<0.001
图3 pORF-hIL-2质粒转染组与空白对照组裸鼠肺转移瘤数目对照**P=0.007
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