Abstract:
Objective To explore the expression of P-gp, GST-π, and TopoⅡ in the malignant tumor of biliary tract and their relationship with the clinical pathological parameters.
Methods The expression of P-gp, GST-π and TopoⅡ proteins in the 42 cases of the malignant tumors of biliary tract were tested by immunohistochemistry of peroxidase labeled streptavidin egg sensitized(SP). This work also made an aggregated analysis combining the P-gp, GST-π and TopoⅡ with the clinical pathological parameters and the mutual relationship between the three of them.
Results The positive expression rates of P-gp, GST-π and TopoⅡ in the malignant tumors of biliary tract were 88.1%, 92.9% and 73.8%. The expression of P-gp and GST-π weren't associated with the age, gender, degree of differentiation, the depth of invasion and lymph node metastasis. The expression of TopoⅡ wasn't associated with the clinical pathology factors except the degree of differentiation (P<0.05). There was a negative correlation between the expression of TopoⅡ and degree of differentiation. The expression of P-gp and GST-π had positive correlation(P<0.05). But there wasn't any correlation between the three index.
Conclusions There are high expression of P-gp, GST-π and TopoⅡ in the malignant tumors of biliary tract, which hints that there is a high primary drug resistance phenomenon on the patients who haven't received any chemotherapy. It is of great significance of detecting aggregated expression of three drug resistance protein for the guiding clinical chemotherapy.
Key words:
Malignant tumor, biliary tract,
P-glycoprotein,
TopoisomeraseⅡ,
Glutathione-s-transferases-π
Yan-ming DONG, Kai HUANG, Xing-wu YANG, Chun-ming YANG. Expression of P-gp, GST-π and TopoⅡ in the malignant tumors of biliary tract and its clinical significance[J]. Chinese Archives of General Surgery(Electronic Edition), 2010, 04(01): 16-19.