To assess the safety and efficacy of applying enhanced recovery after surgery （ERAS） into the perioperative management of patients undergoing hepatectomy for hepatic cystic echinococcosis （HCE）.
Methods
A retrospective analysis of 140 patients treated with hepatectomy for HCE in the First Affiliated Hospital of Xinjiang Medical University from January 2014 to January 2023 was conducted. Based on the mature promotion of the ERAS concept during HCE surgery as timeline， patients were divided into the ERAS group （79 patients treated with ERAS） and the traditional group （61 patients treated traditionally）. A comparative analysis was made of both groups’ general conditions， operation time，intraoperative blood loss， postoperative indicators， pre- and postoperative laboratory examination results， and complication rates.
Results
There were no statistically significant differences in the general conditions of the two groups. The ERAS group showed no significant difference compared to the traditional group in terms of operation time and intraoperative blood loss. However， the ERAS group performed better in terms of first postoperative flatus time， postoperative pain scores （at 24 h and 48 h）， postoperative hospital stay， drainage tube indwelling time， hospitalization costs， and patient satisfaction （all P＜0.05）. In terms of laboratory results， postoperative white blood cells and procalcitonin levels were lower in the ERAS group （P＜0.05）. The overall complication rate in the two groups showed no significant difference.
Conclusions
Application of the ERAS concept in perioperative management of HCE hepatectomy is safe and effective， which not only does not increase the risk of postoperative complications， but also significantly alleviates postoperative pains，shortens hospital stay， and reduces hospitalization costs， thereby accelerating postoperative recovery. The widespread promotion of the ERAS concept in clinical practice is recommended.
To investigate the function and molecular mechanism of LINC00520 in gastric cancer and to reveal the potential role of LINC00520 in gastric cancer development.
Methods
GEO database were screened for differentially expressed genes associated with malignant progression of gastric cancer. qPCR was performed to detect the expression of LINC00520 in gastric cancer， and cellular function experiments were performed to explore the potential effects of LINC00520. The association between LINC00520 and miR-519b-3p and HIF1A was detected by bioinformatics analysis， and their binding activities were verified by using dual luciferase reporter gene and RNA immunoprecipitation assays.
Results
In gastric cancer， the expression of LINC00520 was significantly increased （P＜0.01）， and LINC00520 promoted the malignant progression of gastric cancer cells （P＜0.01）. By bioinformatics， dual luciferase reporter gene and RNA immunoprecipitation assays， it was found that LINC00520 upregulated HIF1A expression by competitively targeting miR-519b-3p （P＜0.01）.
Conclusion
High expression of LINC00520 in gastric cancer plays a critical role by targeting the miR-519b-3p/HIF1A axis， which provides new targets for the diagnosis and treatment of gastric cancer.
To compare the long-term treatment outcomes of robotic-assisted natural orifice specimen extraction surgery （NOSES） with laparoscopic-assisted surgery in patients with mild-tolow risk rectal cancer.
Methods
A retrospective analysis was conducted in 64 patients with middle to low rectal cancer who underwent NOSES treatment in the 940th Hospital of the Joint Logistic Support Force between June 2018 and June 2021. 20 cases （R-N group） received robotic assistance surgery， while 44 cases（L-N group） underwent laparoscopic-assisted surgery. The follow-up period extended until September 2022 and was conducted via phone or outpatient visits. The study compared basic demographics， intraoperative conditions， postoperative complications， and three-year disease-free survival （DFS） and overall survival （OS）between the two groups.
Results
The R-N group had significantly higher hospitalization costs and longer operation time compared to the L-N group （P＜0.001）. However， intraoperative blood loss was significantly less in the R-N group （P=0.001）. Additionally， the R-N group experienced shorter first postoperative flatus time （P=0.016） and lower usage of postoperative analgesics， including parecoxib sodium （P=0.002）. There were no significant differences in postoperative complications and pathological outcomes between the two groups. The three-year OS （90.0% vs 90.9%， P=0.707） and DFS （80.0% vs 86.4%， P=0.892） were similar between both groups.
Conclusion
Compared to laparoscopic-assisted surgery combined with NOSES for middle to low rectal cancer， robotic-assisted surgery combined with NOSES offers higher safety and efficacy，reduced intraoperative blood loss， fewer postoperative complications， and potentially better patient prognosis.
To test the distribution of thyroid stimulating hormone receptor （TSHR）gene 727 codon polymorphism （p.Asp727Glu， p.D727E） in nodular goiter （NG）， and explore the occurrence and development of NG with low thyroid stimulating hormone （TSH） level.
Methods
A total of 17 cases of NG with low TSH level were collected as low TSH level group from January 2018 to December 2019 in the Department of Breast-Thyroid Surgery， South Campus of the First People’s Hospital Affiliated to Shanghai Jiao Tong University， and 10 cases of NG with normal TSH level were randomly selected as normal group. DNA was extracted from tissue sections of all 27 patients for whole exon sequencing of TSHR gene.The correlation between TSHR gene D727E polymorphism and clinical data was statistically analyzed.
Results
TSHR D727E GC+GG genotype accounted for 37.0% （10/27） of NG patients. Subgroup analysis showed that the incidence of GC+GG genotype in low TSH level group was significantly higher than that in normal group （52.9% vs 10.0%， P=0.042）. In low TSH level group， GC+GG genotype had a tendency to be older ［（55.00±16.30） years vs （40.38±14.09） years， P=0.060］ and smaller lesions ［（2.34±1.58） cm vs（3.97±2.13） cm， P=0.101］， which was not seen in normal group. In NG patients， males were more likely to be GC+GG genotype （71.4% vs 25.0%， P=0.029）.
Conclusion
The occurrence and development of NG with low TSH level may be associated with TSHR D727E GC+GG genotype.
Phosphoenolpyruvate carboxykinase-1 （ PCK1 ） is one of the key enzymes in the gluconeogenesis pathway in vivo. In recent years， studies have confirmed that PCK1 plays a key regulatory role in the occurrence and development of various tumors such as gastrointestinal tumors， renal clear cell carcinoma， breast cancer， and melanoma. Based on the overview of PCK1， this article focuses on the unique role of PCK1 in tumor differential expression， signal transduction， physiological processes， and regulation of tumor drug sensitivity， aiming to provide new ideas for clinical development of anticancer drugs.
Primary liver cancer is one of the most common malignant tumours in China. Surgical resection is important to achieve long-term survival and even cure for liver cancer patients， but the postoperative recurrence rate is high. Most of the patients are already in the middle or late stage at the time of initial diagnosis and are not suitable for surgical resection， so how to improve the prognosis of liver cancer patients has become a difficult problem that needs to be solved urgently. In recent years， FOLFOX regimen of hepatic arterial infusion chemotherapy （FOLFOX-HAIC）-based conversion and neoadjuvant therapy for hepatocellular carcinoma has improved the survival benefit of hepatocellular carcinoma patients. To clarify the roles of FOLFOX-HAIC-based conversion therapy and neoadjuvant therapy for hepatocellular carcinoma， this article provides a systematic review of the application and research progress of FOLFOXHAIC-based preoperative therapy for hepatocellular carcinoma and discusses the potential advantages of FOLFOX-HAIC-based neoadjuvant therapy for hepatocellular carcinoma.
