Expression and significance of transcription factor Gli2 of Hedgehog signaling pathway in human hepatocellular carcinoma

doi:10.3877/cma.j.issn.1647-0793.2013.01.004

Abstract

Abstract:

Objective

To investigate the expression and clinical significance of downstream transcription factor glioma-associated oncogene homologue 2(Gli2) of Hedgehog(Hh) signaling pathway in human hepatocellular carcinoma(HCC).

Methods

Real time RT-PCR and Western blot were used for detection of mRNA and protein of Gli2 in 20 pairs of HCC tissues and corresponding paraneoplastic liver tissues; Immunohistochemistry was used for detection of Gli2 protein in 52 pairs of HCC tissues and paraneoplastic liver tissues.

Results

mRNA and protein expression of Gli2 in HCC were significantly higher than in paraneoplastic liver tissues. Immunohistochemical results showed that positive expression of Gli2 protein was 38/52(73.1%) in HCC tissues and 8/52(15.4%) in corresponding adjacent tissues, the difference was significant(P<0.01) and the expression of Gli2 protein was correlated with tumor differentiation, vascular invasion and lymph node metastasis(P<0.05).

Conclusion

The abnormal expression of Gli2 is closely related to tumorigenesis, invasion and metastasis of HCC, Gli2 may be an important biological marker of HCC and biological therapeutic target.

Key words: Hepatocellular carcinoma, Hedgehog signaling pathway, Gli2, Transcription factor

Da-wei ZHANG, Liang-qi CAO, Hai-wu LU, Xiao-dong SONG, Song-hang LIU, Ping XUE. Expression and significance of transcription factor Gli2 of Hedgehog signaling pathway in human hepatocellular carcinoma[J]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(01): 12-16.

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https://zhptwkxwx.cma-cmc.com.cn/EN/Y2013/V07/I01/12

Figures/Tables 5

References 19

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目的基因	引物序列(5'-3')	产物长度（bp）
Gli2	上游序列TGGCCGCTTCAGATGACAGATGTTG	200
?	下游序列CGTTAGCCGAATGTCAGCCGTGAAG	?
18s rRNA	上游序列CCTGGATACCGCAGCTAGGA	112
?	下游序列GCGGCGCAATACGAATGCCCC	?

目的基因	引物序列(5'-3')	产物长度（bp）
Gli2	上游序列TGGCCGCTTCAGATGACAGATGTTG	200
?	下游序列CGTTAGCCGAATGTCAGCCGTGAAG	?
18s rRNA	上游序列CCTGGATACCGCAGCTAGGA	112
?	下游序列GCGGCGCAATACGAATGCCCC	?

临床病理特征		Gli2蛋白表达		χ ²值	P值
临床病理特征		阳性(n=38)	阴性(n=14)	χ ²值	P值
性别		?	?	0.044	0.834
?	男	26	10	?	?
?	女	12	4	?	?
年龄(岁)		?	?	0.061	0.805
?	≤60	23	9	?	?
?	>60	15	5	?	?
HBsAg		?	?	0.126	0.722
?	阳性	31	12	?	?
?	阴性	7	2	?	?
AFP(μg/L)		?	?	0.391	0.532
?	≤200	18	8	?	?
?	>200	20	6	?	?
肝硬化		?	?	0.316	0.574
?	Present	30	10	?	?
?	Absent	8	4	?	?
肿瘤大小(cm)		?	?	3.124	0.077
?	≤5	24	5	?	?
?	>5	14	9	?	?
TNM分期		?	?	0.931	0.335
?	I-II	22	6	?	?
?	Ⅲ-Ⅳ	16	8	?	?
分化程度		?	?	3.958	0.047^a
?	高	8	7	?	?
?	中、低	30	7	?	?
包膜		?	?	1.497	0.221
?	完整	12	7	?	?
?	不完整	26	7	?	?
血管侵犯		?	?	6.361	0.012 ^a
?	+	28	5	?	?
?	-	10	9	?	?
淋巴结转移		?	?	5.450	0.020^a
?	+	22	3	?	?
?	-	16	11	?	?

临床病理特征		Gli2蛋白表达		χ ²值	P值
临床病理特征		阳性(n=38)	阴性(n=14)	χ ²值	P值
性别		?	?	0.044	0.834
?	男	26	10	?	?
?	女	12	4	?	?
年龄(岁)		?	?	0.061	0.805
?	≤60	23	9	?	?
?	>60	15	5	?	?
HBsAg		?	?	0.126	0.722
?	阳性	31	12	?	?
?	阴性	7	2	?	?
AFP(μg/L)		?	?	0.391	0.532
?	≤200	18	8	?	?
?	>200	20	6	?	?
肝硬化		?	?	0.316	0.574
?	Present	30	10	?	?
?	Absent	8	4	?	?
肿瘤大小(cm)		?	?	3.124	0.077
?	≤5	24	5	?	?
?	>5	14	9	?	?
TNM分期		?	?	0.931	0.335
?	I-II	22	6	?	?
?	Ⅲ-Ⅳ	16	8	?	?
分化程度		?	?	3.958	0.047^a
?	高	8	7	?	?
?	中、低	30	7	?	?
包膜		?	?	1.497	0.221
?	完整	12	7	?	?
?	不完整	26	7	?	?
血管侵犯		?	?	6.361	0.012 ^a
?	+	28	5	?	?
?	-	10	9	?	?
淋巴结转移		?	?	5.450	0.020^a
?	+	22	3	?	?
?	-	16	11	?	?

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