Mechanism of the preventive effect of a compound Chinese drug of SanYeXiangChaCai on carbon tetrachloride induced hepatic fibrosis

doi:10.3877/cma.j.issn.1674-0793.2007.01.002

Abstract

Abstract:

Objective

To examine the effects of a compound Chinese medicine of SanYeXiangChaCai （Rabdosia ternifold，Prismatomeris tetrandra，Panaxpseudo-ginseng） on the treatment of hepatic fibrosis，and to explore its mechanisms in rat model.

Methods

The rat model of carbon tetrachloride （CCl₄）-induced hepatic fibrosis was used to assess the effect of h SanYeXiangChaCai on the treatment offibrosis.Hepatic fibrosis was evaluated by hematoxylin-eosin staining and Masson's trichrome staining.Hepatic hydroxyproline and serum ALT/AST were examined by biochemistry.The expression of α-SMA in liver tissue was examined by immunohistochemistry.The expression of TGFβ1 and CTGF was assessed by RT-PCR and Western blot analyses.

Results

Histological and hepatic hydroxyproline examination revealed that SanYeXiangChaCai significantly arrested progression of hepatic fibrosis.SanYeXiangChaCai caused significant amelioration of liver injury （reduced activities of serum ALT and AST） and decreased α-SMA expression.Furthermore，SanYeXiangChaCai markedly reduced both mRNA and protein expression of TGF-β1 as well as CTGF.

Conclusions

These results suggest that SanYeXiangChaCai ameliorates carbon tetrachloride-induced hepatic fibrogenesis in rats via inhibition of TGF-β1 and CTGF expression.

Key words: the compound drug of Rabdosia ternifold,Prismatomeris tetrandra,Panaxpseudoginseng, Liver fibrosis, Smooth muscle actin, Transforming growth factor β1, Connective tissue growth factor

Mao-chuan ZHEN, Qian WANG, Xi-lin CHEN, Liang-qi CAO, Xiao-hui HUANG. Mechanism of the preventive effect of a compound Chinese drug of SanYeXiangChaCai on carbon tetrachloride induced hepatic fibrosis[J]. Chinese Archives of General Surgery(Electronic Edition), 2007, 01(01): 4-8.

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Figures/Tables 4

References 15

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	正常对照组	复方三叶香茶菜对照组	纤维化模型组	复方三叶香茶菜干预组
AST（IU/L）	62.3±26.2	63.4±28.4	225.6±62.7^*	142.4±47.6^△
ALT（IU/L）	33.2±6.2	32.4±7.7	165.5±34.5^*	102.4±22.3^△

	正常对照组	复方三叶香茶菜对照组	纤维化模型组	复方三叶香茶菜干预组
AST（IU/L）	62.3±26.2	63.4±28.4	225.6±62.7^*	142.4±47.6^△
ALT（IU/L）	33.2±6.2	32.4±7.7	165.5±34.5^*	102.4±22.3^△

组别	数目	0级	1级	2级	3级	4级	mean ±s
正常对照组	8	8	0	0	0	0	0.00±0.00
复方三叶香茶菜对照组	8	8	0	0	0	0	0.00±0.00
肝纤维化模型组	10	0	0	1	2	7	3.60±0.70^*
复方三叶香茶干预组	12	0	2	6	4	0	2.17±0.72^△

组别	数目	0级	1级	2级	3级	4级	mean ±s
正常对照组	8	8	0	0	0	0	0.00±0.00
复方三叶香茶菜对照组	8	8	0	0	0	0	0.00±0.00
肝纤维化模型组	10	0	0	1	2	7	3.60±0.70^*
复方三叶香茶干预组	12	0	2	6	4	0	2.17±0.72^△

组别	纤维化面积（%）	羟脯氨酸（μg/g）
正常对照组	3.2±0.8	160±18
复方三叶香茶菜对照组	3.1±0.6	164±20
肝纤维化模型组	20.5±5.4a	565±31^*
复方三叶香茶干预组	13.2±3.6b	398±26^△

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