The effect of Caveolin-1 on cell cycle and apoptosis in Hs578T drug-resistant breast cancer

doi:10.3877/cma.j.issn.1674-0793.2007.01.004

Abstract

Abstract:

Objective

Toinvestigate the effects of caveolion-1 gene on growth and apoptosis in Hs578T's doxorubicin-resistant （Hs578T/Dox） breast cancer.

Methods

The plasmid pCI-neocav-1 was introduced into Hs578T/Dox by lipofectin，and then selected single clone cells highly expressing Caveolin-1 level.Transfectants overexpression of Caveolin-1 was checked by Western blot.Cell cycle and apoptosis were analysis by flow cytometry.Cells proliferation were analysis by the soft agar clonogenic assay.

Result

The cell line overexpressed Caveolin-1 was established successfully.Transfected cells decreased markedly in G0/G1 phase （＜0.01） and increased significantly in S and（M+G2） phases （＜0.01），compared with parent Hs578T/Dox cells.overexpression of caveolin-1 enhance colony formation

Conclusion

Caveolin-1 suppresses apoptosis and promotes growth and proliferation in Hs578T/Dox cells.Caveolin-1 also.

Key words: Breast cancer, Caveolin-1, Cell cycle, Apoptosis

Bao-ju QI, Fei LI, Ya-ming ZHENG, Shuang LIU, Hai-chen SUN. The effect of Caveolin-1 on cell cycle and apoptosis in Hs578T drug-resistant breast cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2007, 01(01): 12-14.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2007.01.004

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2007/V01/I01/12

Groups	凋亡率%	G0/G1/%	S/%	G2/M/%
Hs578T/D-cav	4.91	14.3	54.5	31.3
Hs578T/D	7.17	54.1	33.4	12.5

Groups	凋亡率%	G0/G1/%	S/%	G2/M/%
Hs578T/D-cav	4.91	14.3	54.5	31.3
Hs578T/D	7.17	54.1	33.4	12.5

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