Inducing the Long-term survival of rat liver transplantation by recipient Mesenchymal stem cells engraftment in transplanted livers

doi:10.3877/cma.j.issn.1674-0793.2007.02.004

Abstract

Abstract:

Objective

To investigate the role of mesenchymal stem cells in the long-term survival of rat with liver transplantation

Methods

24 recipient Wistar rats were divided into 3 groups:blank-control group(Group A),D-hanks solution group(Group B)and mesenchymal stem cells(MSCs)group(Group C).The liver function(ALT、Tbil)were observed 1 week after the operation.Liver pathological changes and survival time were also observed.Liver repopulation and differentiation were assessed using Gene-Sry in site hybrid histochemistry(ISHH)and Albumin Immunohistochemistry(IHC).

Results

Compared with group A and B,The medium survival time of group C increased significantly(P<0.05).However,ALT,TBil decreased significantly(P<0.05).The group A and B showed more severe acute rejection in 10 days after the operation,but there was little evidence of acute rejection in group C 60 days later after the operation.

Conclusions

The reason of alleviating the acute rejection and improving the long-term survival of rat with liver transplantation might be the mesenchymal stem cells differentiation into bioactive hepatocytes gradually by intraportal transfusion.

Key words: rat, liver transplantation, acute rejection, hepatocyte

Ming-xin PAN, Fan-dong KONG, Yu-qiang SHAN, Yi Gao. Inducing the Long-term survival of rat liver transplantation by recipient Mesenchymal stem cells engraftment in transplanted livers[J]. Chinese Archives of General Surgery(Electronic Edition), 2007, 01(02): 78-80.

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https://zhptwkxwx.cma-cmc.com.cn/EN/Y2007/V01/I02/78

Figures/Tables 2

References 10

1	Starzl TE,Groth CG,Brettschneider L,et al.Orthotopic homotransplantation of the human liver.Ann Surg,1968,168:392～415
2	Jin T,Toki J,Inaba M,et al.A novel strategy for organ allografts using sublethal(7Gy)irradiation followed by injection of donor bone marrow cells via portal vein.Transplantation,2001,71:1725～1731
3	Kamada N,Calne RY.Orthotopic liver transplantation in the rat techniquess using cuff for portal vein anastomosis and biliary drainage.Transplantation 1979,28(1):47～50
4	Hale DA,Gottschalk R,Umemura A,et al.Establishment of stable multilineage hematopoietic chimerism and donor-specific tolerance without irradiation.Transplantation,2000,69:1242～51
5	苑学礼,谢蜀生,刘家望,等.诱导免疫耐受预防大鼠肾脏移植排斥反应的研究.中华泌尿外科杂志,2001,22:69～71
6	Theise ND,Badve S,Saxena R,et al.Derivation of hepatocytes from bone marrow cells in mice after radiation-induced myeloablation.Hepatology,2000,31:235～240
7	Osawa M,Hanada K,Hamada H,et al.Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell.Science,1996,273:242～245
8	Avital I,Inderbitzin D,Aoki T,et al.Isolation,characterization,and transplantation of bone marrow-derived hepatocyte stem cells.Biochem Biophys Res Commun,2001,288:156～164
9	Alison MR,Poulsom R,Jeffery R,et al.Hepatocytes from nonhepatic adult stem cells.Nature,2000,406:257
10	Theise ND,Nimmakayalu M,Gardner R,et al.Liver from bone marrow in humans.Hepatology,2000,32(1):11～16

组别	n	生存期(天)	MST(天)
A	8	10,12,11,10,12,11,10,12	11
B	8	10,10,9,11,10,9,11,10	10^*
C	8	68,95,>180(×6)	>180^△

组别	n	生存期(天)	MST(天)
A	8	10,12,11,10,12,11,10,12	11
B	8	10,10,9,11,10,9,11,10	10^*
C	8	68,95,>180(×6)	>180^△

组别	n	TBil(μmol/L)	ALT(IU/L)
正常值	8	6.50±1.33	36.34±1.3
A	8	52.01±1.67	101.89±3.97
B	8	69.00±2.47	121.82±2.97
C	8	19.45±2.30^*	71.25±1.35I^*

组别	n	TBil(μmol/L)	ALT(IU/L)
正常值	8	6.50±1.33	36.34±1.3
A	8	52.01±1.67	101.89±3.97
B	8	69.00±2.47	121.82±2.97
C	8	19.45±2.30^*	71.25±1.35I^*

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