Abstract:
Objective
To investigate the changes in concentration and distribution of Ca2+ in splenic macrophage (Mφ) in hypersplenism due to portal hypertension (PH), providing the evidence for discussing the changes and the immune function of PH spleen.
Methods
Twelve patients with hypersplenism due to PH and four patients with traumatic rupture of spleen were regarded as hypersplenia and control group respectively.Using Fluo-3/AM as indicator,the concentration and distribution of Ca2+in splenic Mφ were detected by laser confocal microscope and flow cytometry,respectively.
Results
As compared to control group,the concentration of Ca2+in PH spleen Mφ were increased significantly (48.75±2.61 vs 39.92±1.76, P<0.05). There were many pseudopod-like prominency with Fluo-3 fluorescence positive on the surface of PH splenic Mφ.
Conclusions
The concentration of Ca2+in PH spleen Mφis increased significantly.It further supports the viewpoint that the PH splenic Mφretained at activated state.But it still needs to be cleared that the mechanism of changes in the concentration and distribution of Ca2+and its effects on immune function ofPH spleen.
Key words:
Hypertension,portal,
Hypersplenism,
Macrophages,
Ca2+
Shu ZHANG, Zong-fang LI, Jun LI, Chen HUANG, Jin-hai WANG, Song REN, Rui ZHOU, An JIANG. Changes in concentration and distribution of Ca2+in splenic macrophage from hypersplenism due to portal hypertension[J]. Chinese Archives of General Surgery(Electronic Edition), 2009, 03(03): 201-203.