Immunomodulation effect on neutrophil leukocyte and lymphocyte subsets in septic rats

doi:10.3877/cma.j.issn.1674-0793.2009.05.002

Abstract

Abstract:

Objective

To knowledge the mechanism of immunomodulation,we study immunomodulation effect on neutrophil leukocyte and lymphocyte subsets in septic rat.

Methods

Cecal ligation-perforation(CLP)rat model were divided into three groups including sham group(n=20),control group(n=20)and experimental group(n=20).Blood collections were made after CLP model in 3,12,24,48 h respectively.Leukocyte,neutrophil leukocyte,lymphocyte counting and CD₄⁺,CD₈⁺T lymphocyte were detected.

Results

CLP rat as septic model,leukocyte and neutrophil leukocyte in experimental group decreased more significant than that in control group,P＜0.05.Lymphocyte in experimental group increased more significant than that in control group,P＜0.05.CD₄⁺T lymphocyte in experimental group increased more significant than that in control group,P＜0.05.CD₈⁺T lymphocyte had no difference in two groups.

Conclusions

Immunomodulation in septic rat can decrease leukocyte and neutrophil leukocyte and alleviate sequential inflammatory reaction.Immunomodulation in septic rat can increase lymphocyte and CD₄⁺T lymphocyte and improve immune function as a matter of fact.

Key words: Sepsis, Neutrophilleukocyte, Lymphocyte, Tlymphocytesubsets, Immunomodulation

Shunwei HUANG, Xiang-dong GUAN, Juan CHEN, Yang-bin OU, Chun-hua YANG, Yong-jun LIU. Immunomodulation effect on neutrophil leukocyte and lymphocyte subsets in septic rats[J]. Chinese Archives of General Surgery(Electronic Edition), 2009, 03(05): 4-6.

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References 10

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组别	白细胞	中性粒细胞	淋巴细胞	CD₄⁺T淋巴细胞%
假手术组
3h	6.28±0.51	0.33±0.04	5.75±0.32	30.6±2.31
12h	6.60±0.55	0.35±0.03	6.04±0.43	49.4±3.36
24h	5.69±0.31	0.16±0.02	5.39±0.48	44.7±3.52
48h	5.75±0.73	0.17±0.01	5.34±0.60	46.9±2.01
对照组
3h	6.28±0.82	0.33±0.05	5.75±0.34	32.0±1.51
12h	6.96±0.34	1.52±0.05^*	4.50±0.46	31.2±1.68
24h	8.61±0.44^*	0.70±0.03^*	2.42±0.36^*	28.5±1.85
48h	5.12±0.62	1.01±0.03^*	2.17±0.48^*	30.1±1.64
实验组
3h	6.60±0.71	0.35±0.04	6.04±0.40	31.3±2.25
12h	4.76±0.40	0.66±0.06	4.83±0.44	33.3±1.73
24h	5.54±0.51	0.32±0.02	3.92±0.50	44.2±2.53^*
48h	4.50±0.52	0.43±0.05	3.24±0.50	51.0±1.82^*

组别	白细胞	中性粒细胞	淋巴细胞	CD₄⁺T淋巴细胞%
假手术组
3h	6.28±0.51	0.33±0.04	5.75±0.32	30.6±2.31
12h	6.60±0.55	0.35±0.03	6.04±0.43	49.4±3.36
24h	5.69±0.31	0.16±0.02	5.39±0.48	44.7±3.52
48h	5.75±0.73	0.17±0.01	5.34±0.60	46.9±2.01
对照组
3h	6.28±0.82	0.33±0.05	5.75±0.34	32.0±1.51
12h	6.96±0.34	1.52±0.05^*	4.50±0.46	31.2±1.68
24h	8.61±0.44^*	0.70±0.03^*	2.42±0.36^*	28.5±1.85
48h	5.12±0.62	1.01±0.03^*	2.17±0.48^*	30.1±1.64
实验组
3h	6.60±0.71	0.35±0.04	6.04±0.40	31.3±2.25
12h	4.76±0.40	0.66±0.06	4.83±0.44	33.3±1.73
24h	5.54±0.51	0.32±0.02	3.92±0.50	44.2±2.53^*
48h	4.50±0.52	0.43±0.05	3.24±0.50	51.0±1.82^*

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