Abstract:
Objective
To evaluate the feasibility and sensitivity of detecting the mutations of KRAS in colorectal carcinoma patients with high resolution melting curve analysis.
Methods
Direct sequencing was applied to detect the mutations of KRAS codon 12 and 13 of 15 patients with stage III colorectal carcinoma.Then the results were confirmed with high resolution melting curve analysis.
Results
By direct sequencing, 6 of 15 patients(40%)were found to carry KRAS mutations,and the distributions were 2 patients harboring GGT>GAT mutation,2 patients harboring GGT>GTT mutation,1 patient harboring GGC>GAC mutation and 1 patient harboring GGC>GAA mutation.By high resolution melting curve analysis,consistent results of the patients with mutations or without mutations were got.
Conclusions
The new high resolution melting curve analysis is more efficient,more convenient than direct sequencing.Moreover,it is a low-cost test,which is suitable for clinic test.
Key words:
Colorectalneoplasms,
Mutation,
KRASgene,
Highresolutionmeltingcurveanalysis
Xin-hui FU, Yan-hong DENG, Xin-juan FAN, Yan-xin LUO, Dian-ke CHEN, Lei WANG, Ping LAN, Huan-liang LIU, Jian-ping WANG. Detecting KRAS mutations in colorectal carcinoma patients with high resolution melting curve analy－sis[J]. Chinese Archives of General Surgery(Electronic Edition), 2009, 03(05): 14-16.