Control study of therapeutic effect of adipose mesenchymal stem cells and hepatocyte transplanted for hepatic cirrhosis model of rat

doi:10.3877/cma.j.issn.1674-0793.2011.01.004

Abstract

Abstract:

Objective

To evaluate the therapeutic effects of adipose mesenchymal stem cells (ADMSCs) and hepatocyte transplanted for the hepatic cirrhosis model of rats induced by CCL₄.

Methods

Thirty SD rats of hepatic cirrhosis model were randomly divided averagely into two groups and transplanted through Superior Mesenteric vein. Liver functions of rat was examined before and after cell transplantation. HE stain was performed to liver specimen slices. The degeneration and necrosis of hepatic cells and the degree of liver fibrosis were observed under microscope, and further pathological evaluation was made according to observation result.

Results

The liver functions of ADMSCs group was improved significantly in comparison with that of hepatocyte(P<0.05). The transplantation of ADMSCs could inhibit the degeneration and necrosis of hepatic tissue and improve liver fibrosis of the rats. The difference of pathological evaluation between two groups had statistical sense(P<0.05).

Conclusions

The transplantation of ADMSCs through Superior Mesenteric vein has therapeutic effect for the hepatic cirrhosis model of rats. It can improve liver functions and inhibit liver fibrosis.

Key words: Adipose tissue-derived mesenchymal stem cells, Hepatocyte, Transplantation, Hepatic cirrhosis

Xian-hong XIANG, Jia-ping LI, Guo-sheng TAN, Liu-qing CHEN, Wei CHEN, Jian-Yong YANG. Control study of therapeutic effect of adipose mesenchymal stem cells and hepatocyte transplanted for hepatic cirrhosis model of rat[J]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(01): 12-16.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2011.01.004

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Figures/Tables 10

References 13

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项目	组别	动物（只）	0d	7d	15 d	30 d
AST（U/L）	1	15	371.5±25.7	422.6±18.4	298.2±17.6	183.2±26.4
AST（U/L）	2	15	388.2±29.7	440.3±17.2	302.2±21.2	312.2±17.9
ALT（U/L）	1	13	617.6±54.6	756.7±35.3	456.7±35.3	301.3±11.8
ALT（U/L）	2	12	603.4±58.6	809.4±18.9	771.4±18.9	503.4±18.9
ALB（g/L）	1	13	20.4±1.8	22.2±1.7	17.2±3.1	21.9±2.1
ALB（g/L）	2	12	19.6±2.0	17.3±2.0	26.3±2.0	20.3±2.0
TBIL（μmol/L）	1	13	15.1±1.9	17.8±3.1	15.3±3.1	16.3±2.2
TBIL（μmol/L）	2	12	14.3±2.2	18.1±2.5	17.1±2.5	17.5±1.5

项目	组别	动物（只）	0d	7d	15 d	30 d
AST（U/L）	1	15	371.5±25.7	422.6±18.4	298.2±17.6	183.2±26.4
AST（U/L）	2	15	388.2±29.7	440.3±17.2	302.2±21.2	312.2±17.9
ALT（U/L）	1	13	617.6±54.6	756.7±35.3	456.7±35.3	301.3±11.8
ALT（U/L）	2	12	603.4±58.6	809.4±18.9	771.4±18.9	503.4±18.9
ALB（g/L）	1	13	20.4±1.8	22.2±1.7	17.2±3.1	21.9±2.1
ALB（g/L）	2	12	19.6±2.0	17.3±2.0	26.3±2.0	20.3±2.0
TBIL（μmol/L）	1	13	15.1±1.9	17.8±3.1	15.3±3.1	16.3±2.2
TBIL（μmol/L）	2	12	14.3±2.2	18.1±2.5	17.1±2.5	17.5±1.5

组别	动物数（只）	肝纤维化	肝细胞变性坏死	脂肪变性
脂肪间质干细胞组	13	3.50±0.31	2.25±0.23	3.52±0.38
成熟肝细胞组	12	2.17±0.45	1.35±0.29	1.48±0.21

组别	动物数（只）	肝纤维化	肝细胞变性坏死	脂肪变性
脂肪间质干细胞组	13	3.50±0.31	2.25±0.23	3.52±0.38
成熟肝细胞组	12	2.17±0.45	1.35±0.29	1.48±0.21

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