Expression and correlation of Twist and E-cadherin in colorectal carcinoma

doi:10.3877/cma.j.issn.1674-0793.2011.02.006

Abstract

Abstract:

Objective

To investigate the expression of Twist and E-cadherin in colorectal carcinoma tissue and its effects on the colorectal carcinoma differentiation, infiltration and metastasis of the colorectal.

Methods

The expression of Twist and E-cadherin in 60 cases of colorectal carcinoma, 20 cases of colorectal adenoma and 20 cases of paracancerous normal tissues was detected using immunohistochemistry.

Results

The positive rates of Twist in colorectal carcinomas(56.7%) were significantly higher than those in colorectal adenoma (10%) and paracancerous normal tissues (0%). The positive rate of E-cadherin in colorectal carcinoma(35%) was significantly lower than that in colorectal adenoma (70%) and paracancerous normal tissue (100%). The abnormal expression of Twist and E-cadherin were significantly correlated with the tumor differentiation, depth of invasion, lymph node metastasis, the TNM stage(P<0.05), while not correlated with age, gender and the size of tumor.

Conclusions

Twist is an important biological marker in infiltration and early metastasis of colorectal. Detection of Twist and E-cadherin may be important to evaluate the metastasis and prognosis of colorectal carcinoma. Twist can be as a potential therapeutic target in colorectal cancer.

Key words: Colorectal carcinoma, Colorectal adenoma, Neoplasm metastasis, Twist, E-cadherin

Shou-xing WANG, Pei-zhong SHANG, Yong HUANG. Expression and correlation of Twist and E-cadherin in colorectal carcinoma[J]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(02): 109-114.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2011.02.006

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2011/V05/I02/109

Figures/Tables 11

References 13

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组织类型	总标本数	Twist阳性				E-cadherin阳性
组织类型	总标本数	标本数	阳性率（%）	χ²值	P值^*	标本数	阳性率（%）	χ²值	P值
癌旁正常黏膜	20	0	0	19.71	<0.01^*	20	100	7.06	<0.01^#
结直肠腺瘤	20	2	10	13.2	<0.01^*	14	70	?	?
结直肠癌	60	34	56.7	?	?	21	35	7.47	<0.01^#

组织类型	总标本数	Twist阳性				E-cadherin阳性
组织类型	总标本数	标本数	阳性率（%）	χ²值	P值^*	标本数	阳性率（%）	χ²值	P值
癌旁正常黏膜	20	0	0	19.71	<0.01^*	20	100	7.06	<0.01^#
结直肠腺瘤	20	2	10	13.2	<0.01^*	14	70	?	?
结直肠癌	60	34	56.7	?	?	21	35	7.47	<0.01^#

临床病理参数		总标本数	Twist阳性				E-cadherin阳性
临床病理参数		总标本数	标本数	阳性率（%）	χ²值	P值	标本数	阳性率（%）	χ²值	P值
年龄（岁）		?	?	?	?	?	?	?	?	?
?	≤60	25	13	52.0	0.380	?	10	40.0	0.471	?
?	>60	35	21	60.0	?	?	11	31.4	?	?
性别		?	?	?	?	?	?	?	?	?
?	男	34	21	61.8	0.830	?	11	32.4	0.242	?
?	女	26	13	50.0	?	?	10	38.5	?	?
肿瘤大小（cm）		?	?	?	?	?	?	?	?	?
?	≤5	25	16	64.0	0.939	?	7	28.0	0.923	?
?	>5	35	18	51.4	?	?	14	40.0	?	?
分化程度		?	?	?	?	?	?	?	?	?
?	高	13	4	30.8	6.844	<0.05	9	69.2	11.210	<0.05
?	中	29	16	55.2	?	?	10	34.5	?	?
?	低	18	14	77.8	?	?	2	11.1	?	?
TNM分期		?	?	?	?	?	?	?	?	?
?	Ⅰ+Ⅱ	32	13	40.6	7.186	<0.05	16	50.0	6.782	<0.05
?	Ⅲ+Ⅳ	28	21	75.0	?	?	5	17.9	?	?
浸润深度		?	?	?	?	?	?	?	?	?
?	未及浆膜层	21	8	38.1	4.538	<0.05	12	57.1	6.963	<0.05
?	浆膜或全层	39	26	66.7	?	?	9	23.1	?	?
淋巴结转移		?	?	?	?	?	?	?	?	?
?	有	24	19	79.2	8.247	<0.05	3	12.5	8.901	<0.05
?	无	36	15	41.7	?	?	18	50.0	?	?

临床病理参数		总标本数	Twist阳性				E-cadherin阳性
临床病理参数		总标本数	标本数	阳性率（%）	χ²值	P值	标本数	阳性率（%）	χ²值	P值
年龄（岁）		?	?	?	?	?	?	?	?	?
?	≤60	25	13	52.0	0.380	?	10	40.0	0.471	?
?	>60	35	21	60.0	?	?	11	31.4	?	?
性别		?	?	?	?	?	?	?	?	?
?	男	34	21	61.8	0.830	?	11	32.4	0.242	?
?	女	26	13	50.0	?	?	10	38.5	?	?
肿瘤大小（cm）		?	?	?	?	?	?	?	?	?
?	≤5	25	16	64.0	0.939	?	7	28.0	0.923	?
?	>5	35	18	51.4	?	?	14	40.0	?	?
分化程度		?	?	?	?	?	?	?	?	?
?	高	13	4	30.8	6.844	<0.05	9	69.2	11.210	<0.05
?	中	29	16	55.2	?	?	10	34.5	?	?
?	低	18	14	77.8	?	?	2	11.1	?	?
TNM分期		?	?	?	?	?	?	?	?	?
?	Ⅰ+Ⅱ	32	13	40.6	7.186	<0.05	16	50.0	6.782	<0.05
?	Ⅲ+Ⅳ	28	21	75.0	?	?	5	17.9	?	?
浸润深度		?	?	?	?	?	?	?	?	?
?	未及浆膜层	21	8	38.1	4.538	<0.05	12	57.1	6.963	<0.05
?	浆膜或全层	39	26	66.7	?	?	9	23.1	?	?
淋巴结转移		?	?	?	?	?	?	?	?	?
?	有	24	19	79.2	8.247	<0.05	3	12.5	8.901	<0.05
?	无	36	15	41.7	?	?	18	50.0	?	?

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