Abstract:

Objective

To study the change of leptin, leptin receptor, CD4+, CD8+ lymphocytes in the mucosa of the small intestine of patients with obstructive jaundice, and to investigate the relationship between leptin and leptin receptor (Ob-R) and intestinal mucosal immune barrier of patients with obstructive jaundice.

Methods

Thirty patients diagnosed as obstructive jaundice in Department of Hepatobiliary Surgery from March 2012 to July 2013 were selected as experimental group. The experimental group were divided into mild jaundice group (≤171 μmol/L, OJ1 group, 17 cases), and moderate to severe jaundice group (＞171 μmol/L, OJ2 group, 13 cases). Twenty-one patients during the same period without obstructive jaundice were selected as negative controls. Immunohistochemistry was performed in detecting the expression of leptin, OB-R and CD4+, CD8+ lymphocytes in all patients.

Results

The expression of leptin,leptin receptor, CD4+ and CD8+ lymphocytes in experimental group were lower than the control group (Lp 1.63±1.25 vs 2.48±1.25; Ob-R 2.63±1.27 vs 3.90±1.00; CD4+ 17.74±4.33 vs 28.33±4.53; CD8+ 11.61±3.36 vs 15.95±3.69; all P ＜0.05). There was negative correlation between bilirubin and leptin, OB-R,CD4+ and CD8+ lymphocytes; there was positive correlation between leptin, OB-R and CD4+ and CD8+lymphocytes.

Conclusions

The expression of leptin, OB-R, CD4+ and CD8+ lymphocytes are declined in intestinal mucosa of patients with obstructive jaundice. Leptin may exert positive effects in intestinal mucosal immune barrier with obstructive jaundice.

Key words: Obstructive jaundice, Leptin, Leptin receptor, CD4+, CD8+