PPARγ signal pathway initiated by 15-deoxy-prostaglandin J2 suppressing the invasion and metastasis of hepatocellular carcinoma

doi:10.3877/cma.j.issn.1674-0793.2015.05.006

Abstract

Abstract:

Objective

To investigate the effect of 15-deoxy-prostaglandin J2 (15-d-PGJ2) on the invasion and metastasis of hepatocellular carcinoma SMMC-7721 cells, as well as its underlying mechanisms.

Methods

Hepatocellular carcinoma SMMC-7721 cells were treated by natural ligand 15-d-PGJ2 with or without antagonist GW9662 of peroxisome proliferator-activated receptor (PPAR). Invasion and metastasis of SMMC-7721 cells was measured by wound healing assay and Transwell chambers. mRNAs of PTEN and MMP-9 were assessed by RT-PCR and proteins by Western Blotting analysis, respectively.

Results

15-d-PGJ2, but not GW9662, inhibited the invasion and metastasis of SMMC-7721 cells, up-regulated PTEN mRNA and protein expression, and down-regulated MMP-9 mRNA and protein expression. However, after SMMC-7721 cells were pretreated for 1 h with GW9662 and exposed to 15-d-PGJ2, the inhibitive effects of 15-d-PGJ2 on invasion and metastasis of cancer cells were reversed.

Conclusion

15-d-PGJ2 increases PTEN expression and decreases MMP-9 expression to suppress the invasion and metastasis of HCC via activating PPAR signal pathway.

Key words: Peroxisome proliferator activated receptor, Hepatocellular carcinoma, PTEN, Invasion and metastasis

Liangqi Cao, Chuang Wang, Ping Xue, Dawei Zhang, Huihong Liang, Zili Shao. PPARγ signal pathway initiated by 15-deoxy-prostaglandin J2 suppressing the invasion and metastasis of hepatocellular carcinoma[J]. Chinese Archives of General Surgery(Electronic Edition), 2015, 09(05): 351-355.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2015.05.006

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2015/V09/I05/351

Figures/Tables 4

References 12

1	Cao LQ, Chen XL, Wang Q, et al. Upregulation of PTEN involved in rosiglitazone-induced apoptosis in human hepatocellular carcinoma cells[J]. Acta Pharmacol Sin, 2007, 28(6): 879-887.
2	曹良启, 汪谦, 陈锡林, 等. 15-脱氧前列腺素J2体外诱导肝癌细胞失巢凋亡的研究[J]. 中华医学杂志, 2007, 87(42): 3001-3005.
3	Valster A, Tran NL, Nakada M, et al. Cell migration and invasion assays[J]. Methods, 2005, 37(2): 208-215.
4	Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real- time quantitative PCR and the 2(-Delta Delta C (T)) Method[J]. Methods, 2001, 25 (4): 402-408.
5	Okazaki I, Inagaki Y. Novel strategies for hepatocellular carcinoma based on MMPs science[J]. Anticancer Agents Med Chem, 2012, 12(7): 753-763.
6	Kuyvenhoven JP, van Hoek B, Blom E, et al. Assessment of the clinical significance of serum matrix metalloproteinases MMP-2 and MMP-9 in patients with various chronic liver diseases and hepatocellular carcinoma[J]. Thromb Haemost, 2003, 89(4): 718- 725.
7	Gao R, Cai C, Gan J, et al. miR-1236 down-regulates alpha-fetoprotein, thus causing PTENγ accumulation, which inhibits the PI3K/Akt pathway and malignant phenotype in hepatoma cells[J]. Oncotarget, 2015, 6(8): 6014-6028.
8	Cao LQ, Wang XL, Wang Q, et al. Rosiglitazone sensitizes hepatocellular carcinoma cell lines to 5-fluorouracil antitumor activity through activation of the PPARgamma signaling pathway[J]. Acta Pharmacol Sin, 2009, 30(9): 1316-1322.
9	曹良启, 汪谦, 陈锡林, 等. 过氧化物酶体增殖物激活受体γ和PTEN在肝癌组织中的表达及意义[J/CD]. 中华普通外科学文献:电子版, 2010, 4 (1): 20-24.
10	Wu J, Hao ZW, Zhao YX, et al. Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma[J]. J Transl Med, 2014, 12: 190.
11	Zhou QL, Yang CX, Liang H, et al. Propofol reduces MMPs expression by inhibiting PI3K/AKT activity in human HepG2 cells[J]. Biomed Pharmacother, 2013.
12	Gao J, Ding F, Liu Q, et al. Knockdown of MACC1 expression suppressed hepatocellular carcinoma cell migration and invasion andinhibited expression of MMP2 and MMP9[J]. Mol Cell Biochem, 2013, 376(1-2): 21-32.

[1]	Miao Li, Lianhua Zhu, Peng Han, Bo Jiang, Xiang Fei. Evaluation of relationsip between vascular morphology and risk factors for hepatocellular carcinoma by high-frame-rate contrast-enhanced ultrasound [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(09): 911-915.
[2]	Jianmin Ding, Zhengyi Qin, Xiang Zhang, Yan Zhou, Hongyu Zhou, Yandong Wang, Xiang Jing. Contrast-enhanced ultrasound versus gadoxetic acid-enhanced magnetic resonance imaging for LI-RADS classification of focal liver lesions ≤3 cm in patients at high risk of hepatocellular carcinoma: a prospective study [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(09): 930-938.
[3]	Zicai Fu, Guandong Dai, Weimin Zhu, Wei Lu, Jianyi Xiong, Daping Wang, Zhenhan Deng. Research progress of peroxisome proliferators-activated receptors in osteoarthritis [J]. Chinese Journal of Joint Surgery(Electronic Edition), 2023, 17(03): 363-367.
[4]	Jie Dong, Song Yang, Hao Yang, Xiang Chen, Wanli Zhang. Relationship between hypermethylation of ACACB gene and clinicopathological factors and survival of hepatocellular carcinoma [J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(06): 433-437.
[5]	Wei Huang, Qi Liu, Liuhua Chen, Chaxiang Teng, Zhejian Ou, Hanxiao Liu, Jiancong Chen, Kunsong Zhang. Identification of a pyroptosis-related lncRNA signature as a novel prognostic biomarker for hepatocellular carcinoma [J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(05): 357-365.
[6]	Rongchang Wang, Qifeng Ou, Jinjie Huang, Caiqin Wang, Qian Wang, Xiaohui Huang. Expression of miR-145-5p in hepatocellular carcinoma and its clinical significance [J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(03): 197-202.
[7]	Xiyan Zheng, Zheng Zhou, Fangping He, Zhiqun Lin, Fei Du, Qin Xie, Shaoping Wang, Xianjie Shi. Risk factors for the prognosis of metabolic syndrome and hepatitis B virus related hepatocellular carcinoma [J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(02): 104-109.
[8]	Qijian Yu, Zhe Yang, Shusen Zheng. Current status and progress of research on prevention of tumor recurrence after liver transplantation for hepatocellular carcinoma [J]. Chinese Journal of Transplantation(Electronic Edition), 2023, 17(03): 171-179.
[9]	Yajuan Cao, Binghua Li, Decai Yu. Conversion therapy combined with robot assisted right hemihepatectomy for a patient with hepatocellular carcinoma through Laennec approach [J]. Chinese Journal of Laparoscopic Surgery(Electronic Edition), 2023, 16(02): 116-119.
[10]	Wenhua Zhang, Cui Tao, Tiansong Hu. Clinicopathological characteristics of pedunculated hepatocellular carcinoma in different sites and its effects on postoperative intrahepatic recurrence and prognosis [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(06): 651-655.
[11]	Baoru Hu, Naijian Shang, Di Gao. DCE-MRI and DWI findings of advanced hepatocellular carcinoma and their correlation with prognosis after immunotherapy [J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(06): 399-403.
[12]	Fengyun Wu, Xin Teng, Lianjuan Liu. Diagnostic value of high-frequency ultrasonography and enhanced magnetic resonance for primary hyperdifferentiated hepatocellular carcinoma of different diameters [J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(06): 404-408.
[13]	Zhongbai Liu, Yongjun Ren. Current status and progress of interventional therapy for hepatocellular carcinoma [J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(02): 111-115.
[14]	Xueqing Huang, Nan Wei, Tianpeng Jiang, Tianzhi An, Min Xu, Shi Zhou. Clinical study of TACE for hepatocellular carcinoma by ultrasound-guided transdistal radial artery approach [J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2023, 11(03): 251-256.
[15]	Wei Liang, Xiaobin Wang, Xiaoyang Hong, Mingyue Cai, Licong Liang, Ye Chen, Peikai Huang, Mingyu Liu, Liteng Lin, Kangshun Zhu. The establishment of recurrence model after microwave ablation in mice with orthotopic hepatocellular carcinoma [J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2023, 11(02): 133-139.

Please choose a citation manager

Content to export