Astragaloside Ⅳ protecting hepatic ischemia-reperfusion injury in rats through the Caspases pathway

doi:10.3877/cma.j.issn.1674-0793.2016.03.002

Abstract

Abstract:

Objective

To explore the protective effect of astragaloside Ⅳ on hepatic ischemia-reperfusion injury (HIRI) in rats.

Methods

Seventy-five SD rats were divided into Sham group (control group) , HIRI group (ischemia-reperfusion group) , and astragaloside Ⅳ groups (low dose group, middle dose group, and high dose group) , to establish the model of rat HIRI. After liver was reperfused with blood for 4 h, 8 h, and 16 h, specimens of blood and liver tissues were collected. Serum alanine aminotransferase (ALT) , aspertate aminotransferase (AST) was detected, the changes of liver cell micro-structure were observed under the optical microscope. Western Blotting and immunohistochemical staining was used to analyze the expression of Caspase-3, 8, 9 protein in liver tissues. The liver cell apoptosis rate was examined by flow cytometry.

Results

The levels of ALT, AST in HIRI group raised and in the drug groups decreased obviously. The liver cell damage significantly reduced in drug groups. Compared to HIRI group, the expression of Caspase-3, 8, 9 and apoptosis rate in high dose group were significantly reduced.

Conclusion

Astragaloside Ⅳ pretreatment can reduce HIRI in rats and its mechanism may be associated with inhibiting the expression levels of Caspase-3, 8, 9 and apoptosis inhibition.

Key words: Immunochemistry, Reperfusion injury, Ischemia, Caspases

Ming Gu, Yubin Xu, Feng Xue, Qiang Li, Xinnong Liu, Peijian Zhang. Astragaloside Ⅳ protecting hepatic ischemia-reperfusion injury in rats through the Caspases pathway[J]. Chinese Archives of General Surgery(Electronic Edition), 2016, 10(03): 168-173.

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Figures/Tables 7

References 15

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组别	例数	4h	t值	P值	8h	t值	P值	16 h	t值	P值
Sham组	5	58.04±1.21	25.14	<0.01	59.43±13.93	26.14	<0.01	56.67±4.56	7.36	<0.01
HIRI组	5	602.71±30.01	-	-	945±28.65	-	-	251.23±18.31	-	-
低剂量组	5	521.35±12.29	3.89	<0.05	654.55±19.58	4.21	<0.05	201.48±16.48	3.69	<0.05
中剂量组	5	252.19±15.39	4.50	<0.05	418.33±17.14	6.35	<0.01	100.58±19.35	2.95	<0.05
高剂量组	5	111.26±10.26	8.36	<0.01	178.99±11.39	19.58	<0.01	80.25±9.48	13.46	<0.01

组别	例数	4h	t值	P值	8h	t值	P值	16 h	t值	P值
Sham组	5	58.04±1.21	25.14	<0.01	59.43±13.93	26.14	<0.01	56.67±4.56	7.36	<0.01
HIRI组	5	602.71±30.01	-	-	945±28.65	-	-	251.23±18.31	-	-
低剂量组	5	521.35±12.29	3.89	<0.05	654.55±19.58	4.21	<0.05	201.48±16.48	3.69	<0.05
中剂量组	5	252.19±15.39	4.50	<0.05	418.33±17.14	6.35	<0.01	100.58±19.35	2.95	<0.05
高剂量组	5	111.26±10.26	8.36	<0.01	178.99±11.39	19.58	<0.01	80.25±9.48	13.46	<0.01

组别	例数	4h	t值	P值	8h	t值	P值	16 h	t值	P值
Sham组	5	162.25±11.25	19.25	<0.01	163.48±1.48	21.78	<0.01	166.24±5.79	12.58	<0.01
低剂量组	5	719.16±1.15	2.96	<0.05	1245.22±2.27	3.15	<0.05	439.18±11.2	3.12	<0.05
中剂量组	5	542.12±21.28	4.32	<0.05	914.22±22.71	12.63	<0.01	201.24±11.25	2.81	<0.05
高剂量组	5	289.28±12.46	12.54	<0.01	412.74±8.24	21.47	<0.01	181.22±12.18	19.35	<0.01
HIRI组	5	989.15±13.25	-	-	1612.14±2.15	-	-	456.25±6.21	-	-

组别	例数	4h	t值	P值	8h	t值	P值	16 h	t值	P值
Sham组	5	162.25±11.25	19.25	<0.01	163.48±1.48	21.78	<0.01	166.24±5.79	12.58	<0.01
低剂量组	5	719.16±1.15	2.96	<0.05	1245.22±2.27	3.15	<0.05	439.18±11.2	3.12	<0.05
中剂量组	5	542.12±21.28	4.32	<0.05	914.22±22.71	12.63	<0.01	201.24±11.25	2.81	<0.05
高剂量组	5	289.28±12.46	12.54	<0.01	412.74±8.24	21.47	<0.01	181.22±12.18	19.35	<0.01
HIRI组	5	989.15±13.25	-	-	1612.14±2.15	-	-	456.25±6.21	-	-

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